[Objectives] To optimize the extraction process of polysaccharides from leaves of Dendrobium officinale. [Methods]The polysaccharides from D. officinale were extracted by internal boiling method. The extraction rate o...[Objectives] To optimize the extraction process of polysaccharides from leaves of Dendrobium officinale. [Methods]The polysaccharides from D. officinale were extracted by internal boiling method. The extraction rate of polysaccharides from D. officinale was studied.Based on the single factor experiment,the L_9( 3~3) orthogonal experiment was designed to optimize the extraction process of polysaccharides from D. officinale. [Results]The optimum process for extracting polysaccharides from D. officinale by internal boiling method was as follows:1. 00 g of D. officinale powder was desorbed with 6. 0 mL of 20% ethanol for 20 min,35. 0 mL of 90℃ water was rapidly added,and the extraction time was 5 min. [Conclusions] Under this condition,the extraction rate of polysaccharides from D. officinale was 12. 31%,which was 3. 75 percentage points higher than that of traditional reflux extraction.展开更多
基金Supported by Key Discipline Independent Project for Chemical Engineering of Guangxi Colleges and Universities(2015KLOG18)Natural Science Foundation Project of Guangxi Zhuang Autonomous Region(2017GXNSFFA198007Key Science and Technology Project of Guangxi(Gui Ke AA17202010)
文摘[Objectives] To optimize the extraction process of polysaccharides from leaves of Dendrobium officinale. [Methods]The polysaccharides from D. officinale were extracted by internal boiling method. The extraction rate of polysaccharides from D. officinale was studied.Based on the single factor experiment,the L_9( 3~3) orthogonal experiment was designed to optimize the extraction process of polysaccharides from D. officinale. [Results]The optimum process for extracting polysaccharides from D. officinale by internal boiling method was as follows:1. 00 g of D. officinale powder was desorbed with 6. 0 mL of 20% ethanol for 20 min,35. 0 mL of 90℃ water was rapidly added,and the extraction time was 5 min. [Conclusions] Under this condition,the extraction rate of polysaccharides from D. officinale was 12. 31%,which was 3. 75 percentage points higher than that of traditional reflux extraction.
