Under the background of globalization,there are severe challenges in the cultivation of the professional skills of nursing staff,especially the English skills of foreign-related nursing,which has become an important p...Under the background of globalization,there are severe challenges in the cultivation of the professional skills of nursing staff,especially the English skills of foreign-related nursing,which has become an important part of the training of nursing talents.At present,most English teaching of foreign-related nursing in higher vocational colleges follows the traditional grammar teaching model.However,as most of the teaching staff for medical English are English teachers who have limited nursing professional knowledge,the industry characteristics of medical English are not fully demonstrated in the teaching process,and the lack of practicality inevitably reduces the quality of medical English teaching.Therefore,the traditional nursing English teaching model in higher vocational colleges can no longer meet the actual teaching needs.There is an urgent need to implement an interdisciplinary team teaching model that completely fits the industry environment of foreign-related nursing English and apply it flexibly to improve the quality of foreign-related nursing English teaching in higher vocational colleges,and lay a solid foundation for professional practice of nursing staff.Based on the perspective of ecolinguistics,this article explores the application strategies of the interdisciplinary team teaching model for foreign-related nursing English in higher vocational colleges,with the view to provide guidelines for the actual teaching of foreign-related nursing English.展开更多
The mammalian brain is heterogeneous, containing billions of neurons and trillions of synapses forming vari- ous neural circuitries, through which sense, movement, thought, and emotion arise. The cellular heterogeneit...The mammalian brain is heterogeneous, containing billions of neurons and trillions of synapses forming vari- ous neural circuitries, through which sense, movement, thought, and emotion arise. The cellular heterogeneity of the brain has made it difficult to study the molecular logic of neural circuitry wiring, pruning, activation, and plasticity, until recently, transcriptome analyses with single cell resolution makes decoding of gene regulatory networks underlying aforementioned circuitry properties possible. Here we report success in per- forming both electrophysiological and whole-genome transcriptome analyses on single human neurons in culture. Using Weighted Gene Coexpression Network Analyses （WGCNA）, we identified gene clusters highly correlated with neuronal maturation judged by electrophysiological characteristics. A tight link between neu- ronal maturation and genes involved in ubiquitination and mitochondrial function was revealed. Moreover, we identified a list of candidate genes, which could potentially serve as biomarkers for neuronal maturation. Coupled electrophysiological recording and single cell transcriptome analysis will serve as powerful tools in the future to unveil molecular logics for neural circuitry functions.展开更多
基金School-level project of Guangdong Yunfu Vocational College of Chinese Medicine:Construction and application of an interdisciplinary team teaching model for higher vocational nursing English from the perspective of ecolinguistics(Project number:2023K08)。
文摘Under the background of globalization,there are severe challenges in the cultivation of the professional skills of nursing staff,especially the English skills of foreign-related nursing,which has become an important part of the training of nursing talents.At present,most English teaching of foreign-related nursing in higher vocational colleges follows the traditional grammar teaching model.However,as most of the teaching staff for medical English are English teachers who have limited nursing professional knowledge,the industry characteristics of medical English are not fully demonstrated in the teaching process,and the lack of practicality inevitably reduces the quality of medical English teaching.Therefore,the traditional nursing English teaching model in higher vocational colleges can no longer meet the actual teaching needs.There is an urgent need to implement an interdisciplinary team teaching model that completely fits the industry environment of foreign-related nursing English and apply it flexibly to improve the quality of foreign-related nursing English teaching in higher vocational colleges,and lay a solid foundation for professional practice of nursing staff.Based on the perspective of ecolinguistics,this article explores the application strategies of the interdisciplinary team teaching model for foreign-related nursing English in higher vocational colleges,with the view to provide guidelines for the actual teaching of foreign-related nursing English.
基金The online version of this article （doi：10.1007/s13238-016-0247-8） contains supplementary material, which is available to authorized users.This work was supported by the National Basic Research Program （973 Program） （No. 2012CB966303）, the National Natural Science Foundation of China （Grant Nos. 81330030, 91319309, and 31271371）, the Science and Technology Department of Yunnan Province （Grant 2012HA013）, the Yunnan Basic Research Projects （Grant 2014FC004）, and the Doctoral Tutor of Education Department of Shanghai, China （Grant 20130072110021 ）, grants from NASAD： BRN ＆ BEHV RES FND 23072, NIH R21：IR21NS095184-01 and finally, a fellowship from China Scholarship Council to X. -Y.C.
文摘The mammalian brain is heterogeneous, containing billions of neurons and trillions of synapses forming vari- ous neural circuitries, through which sense, movement, thought, and emotion arise. The cellular heterogeneity of the brain has made it difficult to study the molecular logic of neural circuitry wiring, pruning, activation, and plasticity, until recently, transcriptome analyses with single cell resolution makes decoding of gene regulatory networks underlying aforementioned circuitry properties possible. Here we report success in per- forming both electrophysiological and whole-genome transcriptome analyses on single human neurons in culture. Using Weighted Gene Coexpression Network Analyses （WGCNA）, we identified gene clusters highly correlated with neuronal maturation judged by electrophysiological characteristics. A tight link between neu- ronal maturation and genes involved in ubiquitination and mitochondrial function was revealed. Moreover, we identified a list of candidate genes, which could potentially serve as biomarkers for neuronal maturation. Coupled electrophysiological recording and single cell transcriptome analysis will serve as powerful tools in the future to unveil molecular logics for neural circuitry functions.
