Lunasin,a novel bioactive peptide,is well-known for its anti-proliferation activity.However,the mechanism of this effect is still poorly reported.Here,synthesized lunasin was used and its anti-proliferative function w...Lunasin,a novel bioactive peptide,is well-known for its anti-proliferation activity.However,the mechanism of this effect is still poorly reported.Here,synthesized lunasin was used and its anti-proliferative function was observed at the concentration of 0.25 mg/m L in human breast cancer cell MDA-MB-231.Conjoint analysis of transcriptome and proteome of MDA-MB-231 cells was further performed.The results demonstrated that cysteinyl aspartate specific proteinase(CASP)3,CASP 7,and CASP 14 were significantly up-regulated after lunasin exposure,together with an increased Bax/Bcl-2 ratio from 22.9 to 210.6,which indicated that caspase-mediated mitochondria intrinsic apoptosis was highly activated.Moreover,lysosomal pathway was signifi cantly suppressed under lunasin exposure,suggesting that lysosome may cooperate with mitochondria to participate in apoptosis.In addition,lunasin also down-regulated genes involved in DNA replication in MDA-MB-231 cells.Overall,our study reveals that the anti-proliferation effect of lunasin peptide might be triggered via the inhibition of DNA replication and cell mitosis,as well as the promotion of lysosome-mitochondrial mediated cell apoptosis.展开更多
基金financially supported by the Agricultural Science and Technology Innovation Program[CAAS-ASTIP-2021-ICS]。
文摘Lunasin,a novel bioactive peptide,is well-known for its anti-proliferation activity.However,the mechanism of this effect is still poorly reported.Here,synthesized lunasin was used and its anti-proliferative function was observed at the concentration of 0.25 mg/m L in human breast cancer cell MDA-MB-231.Conjoint analysis of transcriptome and proteome of MDA-MB-231 cells was further performed.The results demonstrated that cysteinyl aspartate specific proteinase(CASP)3,CASP 7,and CASP 14 were significantly up-regulated after lunasin exposure,together with an increased Bax/Bcl-2 ratio from 22.9 to 210.6,which indicated that caspase-mediated mitochondria intrinsic apoptosis was highly activated.Moreover,lysosomal pathway was signifi cantly suppressed under lunasin exposure,suggesting that lysosome may cooperate with mitochondria to participate in apoptosis.In addition,lunasin also down-regulated genes involved in DNA replication in MDA-MB-231 cells.Overall,our study reveals that the anti-proliferation effect of lunasin peptide might be triggered via the inhibition of DNA replication and cell mitosis,as well as the promotion of lysosome-mitochondrial mediated cell apoptosis.
