Mouse cortical radial glial cells(RGCs)are primary neural stem cells that give rise to cortical oligodendrocytes,astrocytes,and olfactory bulb(OB)GABAergic interneurons in late embryogenesis.There are fundamental gaps...Mouse cortical radial glial cells(RGCs)are primary neural stem cells that give rise to cortical oligodendrocytes,astrocytes,and olfactory bulb(OB)GABAergic interneurons in late embryogenesis.There are fundamental gaps in understanding how these diverse cell subtypes are generated.Here,by combining single-cell RNA-Seq with intersectional lineage analyses,we show that beginning at around E16.5,neocortical RGCs start to generate ASCL1^(+)EGFR^(+)apical multipotent intermediate progenitors(MIPCs),which then differentiate into basal MIPCs that express ASCL1,EGFR,OLIG2,and MKI67.These basal MIPCs undergo several rounds of divisions to generate most of the cortical oligodendrocytes and astrocytes and a subpopulation of OB interneurons.Finally,single-cell ATAC-Seq supported our model for the genetic logic underlying the specification and differentiation of cortical glial cells and OB interneurons.Taken together,this work reveals the process of cortical radial glial cell lineage progression and the developmental origins of cortical astrocytes and oligodendrocytes.展开更多
Adult neural stem cells(a NSCs)primarily exist in two regions:the subventricular zone(SVZ)of the lateral ventricle(LV)and the subgranular zone(SGZ)in the hippocampal dentate gyrus(DG)[1].It has been revealed that a NS...Adult neural stem cells(a NSCs)primarily exist in two regions:the subventricular zone(SVZ)of the lateral ventricle(LV)and the subgranular zone(SGZ)in the hippocampal dentate gyrus(DG)[1].It has been revealed that a NSCs in the SVZ(called type B cells)originate from embryonic NSCs mainly before embryonic day 15.5(E15.5),and are set aside for postnatal neurogenesis after diverging[2,3].展开更多
基金supported by grants from the National Key Research and Development Program of China(2018YFA0108000)the National Natural Science Foundation of China(31630032,31820103006,and 32070971)+1 种基金a Shanghai Municipal Science and Technology Major Project(2018SHZDZX01)ZJLab,and grants from NIH(R01MH094589 and R01NS089777)。
文摘Mouse cortical radial glial cells(RGCs)are primary neural stem cells that give rise to cortical oligodendrocytes,astrocytes,and olfactory bulb(OB)GABAergic interneurons in late embryogenesis.There are fundamental gaps in understanding how these diverse cell subtypes are generated.Here,by combining single-cell RNA-Seq with intersectional lineage analyses,we show that beginning at around E16.5,neocortical RGCs start to generate ASCL1^(+)EGFR^(+)apical multipotent intermediate progenitors(MIPCs),which then differentiate into basal MIPCs that express ASCL1,EGFR,OLIG2,and MKI67.These basal MIPCs undergo several rounds of divisions to generate most of the cortical oligodendrocytes and astrocytes and a subpopulation of OB interneurons.Finally,single-cell ATAC-Seq supported our model for the genetic logic underlying the specification and differentiation of cortical glial cells and OB interneurons.Taken together,this work reveals the process of cortical radial glial cell lineage progression and the developmental origins of cortical astrocytes and oligodendrocytes.
基金supported by the grant from the National Natural Science Foundation of China (31600842)
文摘Adult neural stem cells(a NSCs)primarily exist in two regions:the subventricular zone(SVZ)of the lateral ventricle(LV)and the subgranular zone(SGZ)in the hippocampal dentate gyrus(DG)[1].It has been revealed that a NSCs in the SVZ(called type B cells)originate from embryonic NSCs mainly before embryonic day 15.5(E15.5),and are set aside for postnatal neurogenesis after diverging[2,3].
