[Objective] To research and develop spine date seed healthy edible oil. [Method] Spine date seed oil was extracted by supercritical carbon dioxide extraction technology. And then the molecular distillation technology ...[Objective] To research and develop spine date seed healthy edible oil. [Method] Spine date seed oil was extracted by supercritical carbon dioxide extraction technology. And then the molecular distillation technology was used to reduce the acid value and peroxide value of spine date seed oil. Activated carbon and carclazyte were used to process the decolorization and deodorization treatment using the absorption characteristics. [Result] The light yellow, clear pine date seed oil with shallow taste was obtained, which had an acid value of 2.03 mg/g, peroxide value of 4 mmol/kg; the GC indicated that the oil contained 5.00% of palmitic acid, 2.00% of stearic acid, 40.46% of oleic acid, 47.72% of linoleic acid and 0.39% of linolenic. [Conclusion] The obtained spine date seed oil basically conformed to the national edible oil standard and could be used as edible oil, but its pharmacological properties needed further study.展开更多
Comprehensive Summary,Autophagy is a multi-step cell metabolism process in which cells remove damaged and unwanted materials.During autophagy,autophagosomes fuse with lysosomes to form autophagosomes.Autophagosomal me...Comprehensive Summary,Autophagy is a multi-step cell metabolism process in which cells remove damaged and unwanted materials.During autophagy,autophagosomes fuse with lysosomes to form autophagosomes.Autophagosomal membrane components are recycled from autolysosomes through the autophagosomal components recycling(ACR),while lysosomal components circulate on the autolysosomal surface through the autophagic lysosome reformation(ALR)process.Autolysosomes contain components from autophagosomes and lysosomes.However,whether there is a fusion between autolysosome and autophagosome or lysosome at the organelle level remains unknown.In this study,a pH and viscosity dual-controlled mitochondria-targeting fluorescent probe Mito-Q was designed based on an asymmetric norcyanine to achieve the high-contrast imaging of mitochondria-containing autolysosomes.Mito-Q not only effectively detected mitochondrial viscosity changes and mitophagy with high sensitivity,but more importantly,the fusion of mitochondria-containing autolysosomes and autophagosomes(FMAA)was observed during autophagy by the real-time confocal imaging of HeLa cells.展开更多
文摘[Objective] To research and develop spine date seed healthy edible oil. [Method] Spine date seed oil was extracted by supercritical carbon dioxide extraction technology. And then the molecular distillation technology was used to reduce the acid value and peroxide value of spine date seed oil. Activated carbon and carclazyte were used to process the decolorization and deodorization treatment using the absorption characteristics. [Result] The light yellow, clear pine date seed oil with shallow taste was obtained, which had an acid value of 2.03 mg/g, peroxide value of 4 mmol/kg; the GC indicated that the oil contained 5.00% of palmitic acid, 2.00% of stearic acid, 40.46% of oleic acid, 47.72% of linoleic acid and 0.39% of linolenic. [Conclusion] The obtained spine date seed oil basically conformed to the national edible oil standard and could be used as edible oil, but its pharmacological properties needed further study.
基金the National Key Research and Development Program of China(2019YFA0210500)for H.S.the National Natural Science Foundation of China(No.31900033 to H.s.)forfinancial support.
文摘Comprehensive Summary,Autophagy is a multi-step cell metabolism process in which cells remove damaged and unwanted materials.During autophagy,autophagosomes fuse with lysosomes to form autophagosomes.Autophagosomal membrane components are recycled from autolysosomes through the autophagosomal components recycling(ACR),while lysosomal components circulate on the autolysosomal surface through the autophagic lysosome reformation(ALR)process.Autolysosomes contain components from autophagosomes and lysosomes.However,whether there is a fusion between autolysosome and autophagosome or lysosome at the organelle level remains unknown.In this study,a pH and viscosity dual-controlled mitochondria-targeting fluorescent probe Mito-Q was designed based on an asymmetric norcyanine to achieve the high-contrast imaging of mitochondria-containing autolysosomes.Mito-Q not only effectively detected mitochondrial viscosity changes and mitophagy with high sensitivity,but more importantly,the fusion of mitochondria-containing autolysosomes and autophagosomes(FMAA)was observed during autophagy by the real-time confocal imaging of HeLa cells.
