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过表达组蛋白H3K9me3去甲基化酶对猪克隆胚胎发育的影响
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作者 李亚楠 张贤君 +5 位作者 张宁 梁雅琳 张宇星 招华兴 李紫聪 黄思秀 《遗传》 CAS CSCD 北大核心 2023年第1期67-77,共11页
组蛋白异常修饰是克隆胚胎发育的重要制约因素,组蛋白H3K9me3去甲基化酶KDM4家族的过表达可以有效提高克隆胚胎的发育效率。为探究过表达H3K9me3去甲基化酶对猪克隆胚胎发育的影响,本研究在猪克隆胚胎1-细胞期和2-细胞期分别注射KDM4A m... 组蛋白异常修饰是克隆胚胎发育的重要制约因素,组蛋白H3K9me3去甲基化酶KDM4家族的过表达可以有效提高克隆胚胎的发育效率。为探究过表达H3K9me3去甲基化酶对猪克隆胚胎发育的影响,本研究在猪克隆胚胎1-细胞期和2-细胞期分别注射KDM4A mRNA和KDM4D mRNA检测胚胎的囊胚率;收集1-细胞期注射KDM4A mRNA和胚胎注射水(对照组)的2-细胞期克隆胚胎检测H3K9me3表达水平;此外,收集1-细胞期注射KDM4A mRNA和胚胎注射水的4-细胞期克隆胚胎进行单细胞转录组测序,并对测序数据进行GO与KEGG富集分析。结果显示:在1-细胞期注射KDM4A mRNA的猪克隆胚胎囊胚率显著高于对照组(25.32±0.74%vs 14.78±0.87%),注射KDM4D mRNA对猪克隆胚胎囊胚率无明显作用(16.27±0.77%vs 14.78±0.87%);在2-细胞期注射KDM4A mRNA和KDM4D mRNA的克隆胚胎囊胚率与对照组相比均无显著差异(32.18±1.67%、30.04±0.91%vs 31.22±1.40%)。在1-细胞期注射KDM4A mRNA的克隆胚胎组蛋白H3K9me3表达水平低于对照组。通过测序,筛选出133个差异表达基因,其中上调基因52个、下调基因81个,GO分析主要富集到与蛋白质定位相关的通路,KEGG分析富集到细胞衰老和急性髓细胞白血病等相关通路。本研究结果表明,过表达组蛋白H3K9me3去甲基化酶KDM4A可以显著提高猪克隆胚胎的发育效率。 展开更多
关键词 克隆胚胎 H3K9me3 KDM4A KDM4D
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哺乳动物毛色调控机制及其适应性进化研究进展 被引量:17
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作者 章誉兴 吴宏 于黎 《遗传》 CAS CSCD 北大核心 2021年第2期118-133,共16页
哺乳动物类群呈现出的丰富毛色是引人注目的一种生物现象,是研究和理解哺乳动物适应性进化的理想模型之一。哺乳动物的毛色多态在躲避天敌、捕食、求偶及抵御紫外线等方面都具有重要作用。哺乳动物毛发的色素化过程由体内黑色素的数量... 哺乳动物类群呈现出的丰富毛色是引人注目的一种生物现象,是研究和理解哺乳动物适应性进化的理想模型之一。哺乳动物的毛色多态在躲避天敌、捕食、求偶及抵御紫外线等方面都具有重要作用。哺乳动物毛发的色素化过程由体内黑色素的数量、质量和分布状况所决定。黑色素的形成过程复杂,包括黑素细胞的分化、成熟,黑素体等细胞器的形态发生及黑色素在黑素细胞中的合成代谢和转运等过程;而在细胞色素化的每个阶段/时相都伴随着一些重要功能基因的参与,并通过基因之间的相互作用形成了黑色素生物代谢的复杂调控网络,进而形成不同的毛色有助于哺乳动物适应不同生存环境。对哺乳动物不同毛色形成机制的探究一直以来都是遗传学及进化生物学的重要研究领域和聚焦热点。本文综述了哺乳动物毛色色素化过程的主要分子机制以及毛色适应性进化的遗传基础,以期为哺乳动物毛色多态及其适应性进化的分子机制研究提供参考。 展开更多
关键词 毛色 黑色素生成 遗传基础 适应性进化
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The Research of Carbon Emission and Carbon Sequestration Potential of Forest Vegetation in China
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作者 yuxing zhang Jingping YIN +2 位作者 Yao FU Xuejun WANG Guosheng WANG 《Meteorological and Environmental Research》 CAS 2021年第6期24-31,共8页
The study of China s carbon emissions and carbon sequestration potential is of great practical significance to the formulation of carbon neutrality strategies and methods of China.Carbon emission is an inevitable outc... The study of China s carbon emissions and carbon sequestration potential is of great practical significance to the formulation of carbon neutrality strategies and methods of China.Carbon emission is an inevitable outcome of the initial stage of economic development,especially the right of developing countries existence and development.The carbon emission of China has been maintained at a low level for a long time and reached the top of the world in 2005.However,per capita carbon emission was still only 46.28%of the United States in 2016.China s total CO_(2) emissions are expected to reach 17-19 Gt/a by 2030.To achieve the goal of carbon neutrality in 2060,the main problems are the low technical capacity of emission reduction and the unreasonable structure of energy consumption.Therefore,replacing coal with gas is one of the most effective ways of emission reduction.By 2060,the carbon sequestration capacity of forest vegetation will reach or exceed 759.14 Mt/a and the CO_(2) sequestration capacity will reach 2783.5 Mt/a.According to that,China s carbon intensity must reduce by 95.39%on the basis of the carbon intensity in 2017,reaching 640 t/100 million yuan.The carbon sequestration capacity of terrestrial vegetation(forest,grassland)will reach or exceed 1380.3 Mt/a and the CO_(2) sequestration capacity will reach 5069.3 Mt/a.According to that,China s carbon intensity must reduce by 91.07%on the basis of the carbon intensity in 2017,reach 1152 t C per 100 million yuan. 展开更多
关键词 Forest carbon sink Carbon storage Forest resources inventory Carbon neutrality
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Opportunities and challenges in studies of mammalian hybrid speciation
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作者 Hong Wu yuxing zhang Li Yu 《Science China(Life Sciences)》 SCIE CAS CSCD 2024年第3期614-617,共4页
Revealing the mechanisms of speciation remains one of the most attractive and challenging issues in the field of evolutionary biology, and is also key to understanding the biodiversity present in nature.
关键词 BIODIVERSITY ATTRACTIVE EVOLUTIONARY
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The Circadian System Is Essential for the Crosstalk of VEGF-Notch-mediated Endothelial Angiogenesis in Ischemic Stroke 被引量:1
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作者 yuxing zhang Xin Zhao +7 位作者 Chun Guo Ying zhang Fukang Zeng Qian Yin Zhong Li Le Shao Desheng Zhou Lijuan Liu 《Neuroscience Bulletin》 SCIE CAS CSCD 2023年第9期1375-1395,共21页
Ischemic stroke is a major public health problem worldwide.Although the circadian clock is involved in the process of ischemic stroke,the exact mechanism of the circadian clock in regulating angiogenesis after cerebra... Ischemic stroke is a major public health problem worldwide.Although the circadian clock is involved in the process of ischemic stroke,the exact mechanism of the circadian clock in regulating angiogenesis after cerebral infarction remains unclear.In the present study,we determined that environmental circadian disruption(ECD)increased the stroke severity and impaired angiogenesis in the rat middle cerebral artery occlusion model,by measuring the infarct volume,neurological tests,and angiogenesis-related protein.We further report that Bmal1 plays an irreplaceable role in angiogenesis.Overexpression of Bmal1 promoted tube-forming,migration,and wound healing,and upregulated the vascular endothelial growth factor(VEGF)and Notch pathway protein levels.This promoting effect was reversed by the Notch pathway inhibitor DAPT,according to the results of angiogenesis capacity and VEGF pathway protein level.In conclusion,our study reveals the intervention of ECD in angiogenesis in ischemic stroke and further identifies the exact mechanism by which Bmal1 regulates angiogenesis through the VEGF-Notch1 pathway. 展开更多
关键词 Circadian clock Ischemic stroke ANGIOGENESIS VEGF Notch pathway
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High Strength and Excellent Ductility of AZ80 Magnesium Alloy Cabin Component Developed by W-Shaped Channel Extrusion and Subsequent T6 Heat Treatment
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作者 yuxing zhang Zhen Wang +6 位作者 Shuchang Li Xi Zhao Zhimin zhang Yaojin Wu Xianwei Ren Fafa Yan Beibei Dong 《Acta Metallurgica Sinica(English Letters)》 SCIE EI CAS CSCD 2023年第5期839-856,共18页
The AZ80 magnesium(Mg)alloy cabin component with high strength and excellent ductility was developed by W-shaped channel extrusion(WCE)at 350℃ and subsequent T6 heat treatment.The effect of WCE process on the microst... The AZ80 magnesium(Mg)alloy cabin component with high strength and excellent ductility was developed by W-shaped channel extrusion(WCE)at 350℃ and subsequent T6 heat treatment.The effect of WCE process on the microstructure and mechanical properties of the alloy was experimentally investigated,and the age-hardening behavior with microstructure evolution during heat treatment was revealed.Due to the introduction of multi-stage asymmetric extrusion and severe shear deformation along the annular channel,the average grain size of the WCE extruded alloy could be effectively refined to 4.7μm.Besides,theβphase particles were dynamically precipitated from the fine grain boundaries during extrusion,which hindered the grain growth,but worsen the material plasticity.After T6 treatment,the properties of component were eventually improved to a yield strength(YS)of 218 MPa and ultimate tensile strength(UTS)of 344 MPa with elongation(EL)of 14.5%.It was revealed that the rod/lath-and needle-shaped continuousβphase(CP)with finer size precipitated after T6 treatment was more effective in hindering the movement of dislocations and strengthened the alloy than lamellar discontinuousβphase(DP).The dispersed phase precipitated in the grains,the annihilation of dislocations,the uniformly distributed grains and the re-dissolution ofβphase particles at initial grain boundaries after T6 treatment greatly contributed to the ductility of alloy.Moreover,the T6 treatment also promoted the basal plane of most grains which were re-arranged to the extrusion direction,which promoted the possibility of non-basal slip activation and further improved the elongation of the alloy.As a result,the UTS and YS of the final component increased by 10%and the EL increased by 7%,respectively. 展开更多
关键词 AZ80 Mg alloy W-shaped channel extrusion Heat treatment Mechanical properties
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An elastic electrode model for wave propagation analysis in piezoelectric layered structures of film bulk acoustic resonators 被引量:3
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作者 Feng Zhu yuxing zhang +1 位作者 Bin Wang Zhenghua Qian 《Acta Mechanica Solida Sinica》 SCIE EI CSCD 2017年第3期263-270,共8页
Wave propagation in a piezoelectric layered structure of a film bulk acoustic resonator (FBAR/ is studied. The accurate results of dispersion relation are calculated using the proposed elastic electrode model for bot... Wave propagation in a piezoelectric layered structure of a film bulk acoustic resonator (FBAR/ is studied. The accurate results of dispersion relation are calculated using the proposed elastic electrode model for both electroded and unelectroded layered plates. The differences of calculated cut-off frequencies between the current elastic electrode model and the simplified inertial electrode model (often used in the quartz resonator analysis) are illustrated in detail, which shows that an elastic electrode model is indeed needed for the accurate analysis of FBAR. These results can be used as an accurate criterion to calibrate the 2-D theoretical model for a real finite-size structure of FBAR. 展开更多
关键词 FBARs Elastic electrode Waves propagation Dispersion curves Inertial electrode
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