The impact sensitivity assessment of spacecraft is to obtain the probability of spacecraft encountering the OD/M(orbital debris or meteoroid),which is a prerequisite for survivability assessment of on-orbit spacecraft...The impact sensitivity assessment of spacecraft is to obtain the probability of spacecraft encountering the OD/M(orbital debris or meteoroid),which is a prerequisite for survivability assessment of on-orbit spacecraft.An impact sensitivity assessment method of spacecraft based on virtual exterior wall was proposed to improve the computational efficiency.This method eliminates determination of the outermost surface elements of the spacecraft before generating the debris rays,which are assumed to originate from a non-concave virtual wall that completely wraps the spacecraft.The Dist Mesh method was adopted for the generating of the virtual wall to ensure its mesh quality.The influences of the sizes,mesh densities,shapes of the virtual wall on the efficiency and accuracy were considered to obtain the best combination of the size and mesh density of the wall and spacecraft.The results of this method were compared with those of S3DE(Survivability of Spacecraft in Space Debris Environment),BUMPER,MDPANTO,ESABASE2/Debris to verify the feasibility of the method.The PCHIP(Piecewise Cubic Hermite Interpolating Polynomial)was used to fit the size vs.flux relationship of the space debris to acquire the impact probability of OD/M with arbitrary size on the spacecraft.展开更多
The controllable construction of two-dimensional(2D)metal–organic framework(MOF)nanosheets with favorable electrochemical performances is greatly challenging for energy storage.Here,we design an in situ induced growt...The controllable construction of two-dimensional(2D)metal–organic framework(MOF)nanosheets with favorable electrochemical performances is greatly challenging for energy storage.Here,we design an in situ induced growth strategy to construct the ultrathin carboxylated carbon nanotubes(C-CNTs)interpenetrated nickel MOF(Ni-MOF/C-CNTs)nanosheets.The deliberate thickness and specific surface area of novel 2D hybrid nanosheets can be effectively tuned via finely controlling C-CNTs involvement.Due to the unique microstructure,the integrated 2D hybrid nanosheets are endowed with plentiful electroactive sites to promote the electrochemical performances greatly.The prepared Ni-MOF/C-CNTs nanosheets exhibit superior specific capacity of 680 C g^−1 at 1 A g^−1 and good capacity retention.The assembled hybrid device demonstrated the maximum energy density of 44.4 Wh kg^−1 at a power density of 440 W kg^−1.Our novel strategy to construct ultrathin 2D MOF with unique properties can be extended to synthesize various MOF-based functional materials for diverse applications.展开更多
Although the appearance of Doxil alleviated the cardiotoxicity of DOX, the progression-free survival of patients was not prolonged compared with traditional medication regimens, and side effects such as hand-foot synd...Although the appearance of Doxil alleviated the cardiotoxicity of DOX, the progression-free survival of patients was not prolonged compared with traditional medication regimens, and side effects such as hand-foot syndrome has occurred. In order to solve this dilemma, we have designed a novel co-delivery strategy to construct a co-loaded liposome of berberine(BER) and doxorubicin(DOX), which was called Lipo Be Do. The optimal synergistic ratio of the two drugs was screened by cell cytotoxicity experiments in vitro, and the optimal attenuation ratio was further determined by in vivo cardiac H&E staining pathological sections. The optimal combination treatment caused a robust increase in apoptotic cells of 4T1, as compared to drug alone treatment. The prepared co-loaded liposome, Lipo Be Do, had high encapsulation efficiency and good stability. The nanoliposome carrier controlled the biological fate of the drugs and maintained a pre-defined optimal ratio in vivo. The Lipo Be Do significantly inhibited tumor growth in 4T1 murine mammary carcinoma model compared with Doxil(P < 0.05), and completely overcame the myocardial rupture toxicity caused by Doxil in mice. Our co-loaded liposome delivery platform technology provided a new direction for the clinical treatment of triple-negative breast cancer and the safe application of DOX.展开更多
Background:The pig is an economically important livestock species and is a widely applied large animal model in medical research.Enhancers are critical regulatory elements that have fundamental functions in evolution,...Background:The pig is an economically important livestock species and is a widely applied large animal model in medical research.Enhancers are critical regulatory elements that have fundamental functions in evolution,development and disease.Genome-wide quantification of functional enhancers in the pig is needed.Results:We performed self-transcribing active regulatory region sequencing(STARR-seq)in the porcine kidney epithelial PK15 and testicular ST cell lines,and reliably identified 2576 functional enhancers.Most of these enhancers were located in repetitive sequences and were enriched within silent and lowly expressed genes.Enhancers poorly overlapped with chromatin accessibility regions and were highly enriched in chromatin with the repressive histone modification H3K9me3,which is different from predicted pig enhancers detected using ChIP-seq for H3K27ac or/and H3K4me1 modified histones.This suggests that most pig enhancers identified with STARR-seq are endogenously repressed at the chromatin level and may function during cell type-specific development or at specific developmental stages.Additionally,the PPP3CA gene is associated with the loin muscle area trait and the QKI gene is associated with alkaline phosphatase activity that may be regulated by distal functional enhancers.Conclusions:In summary,we generated the first functional enhancer map in PK15 and ST cells for the pig genome and highlight its potential roles in pig breeding.展开更多
In this study, a new on-line health monitoring technology for the determination of the penetration of environment solution into epoxy resin was pursued. A corrosion sensor including plastic optical fiber and pH indica...In this study, a new on-line health monitoring technology for the determination of the penetration of environment solution into epoxy resin was pursued. A corrosion sensor including plastic optical fiber and pH indicator was fabricated. The color-change layer of this sensor appeared after immersion in sulfuric acid solution, which could be examined by using optical fiber and spectrophotometer. The results showed that the penetration of sulfuric acid was detected by adding bromophenol blue （BPB） in the corrosion sensor. This system could be applied to on-line health monitoring of chemical equipment structures.展开更多
A common approach in therapeutic protein development involves employing synthetic ligands with multivalency,enabling sophisticated control of signal transduction.Leveraging the emerging concept of liquid-liquid phase ...A common approach in therapeutic protein development involves employing synthetic ligands with multivalency,enabling sophisticated control of signal transduction.Leveraging the emerging concept of liquid-liquid phase separation(LLPS)and its ability to organize cell surface receptors into functional compartments,we herein have designed modular ligands with phase-separation modalities to engineer programmable interreceptor communications and precise control of signal pathways,thus inducing the rapid,potent,and specific apoptosis of tumor cells.Despite their simplicity,these“triggers”,named phase-separated Tumor Killers(hereafter referred to as psTK),are sufficient to yield interreceptor clustering of death receptors(represented by DR5)and tumor-associated receptors,with notable features:LLPSmediated robust high-order organization,well-choreographed conditional activation,and broad-spectrum capacity to potently induce apoptosis in tumor cells.The development of novel therapeutic proteins with phase-separation modalities showcases the power of spatially reorganizing signal transduction.This approach facilitates the diversification of cell fate and holds promising potential for targeted therapies against challenging tumors.展开更多
As an essential tool for realistic description of the current or future debris environment,the Space Debris Environment Engineering Model(SDEEM)has been developed to provide support for risk assessment of spacecraft.I...As an essential tool for realistic description of the current or future debris environment,the Space Debris Environment Engineering Model(SDEEM)has been developed to provide support for risk assessment of spacecraft.In contrast with SDEEM2015,SDEEM2019,the latest version,extends the orbital range from the Low Earth Orbit(LEO)to Geosynchronous Orbit(GEO)for the years 1958-2050.In this paper,improved modeling algorithms used by SDEEM2019 in propagating simulation,spatial density distribution,and spacecraft flux evaluation are presented.The debris fluxes of SDEEM2019 are compared with those of three typical models,i.e.,SDEEM2015,Orbital Debris Engineering Model 3.1(ORDEM 3.1),and Meteoroid and Space Debris Terrestrial Environment Reference(MASTER-8),in terms of two assessment modes.Three orbital cases,including the Geostationary Transfer Orbit(GTO),Sun-Synchronous Orbit(SSO)and International Space Station(ISS)orbit,are selected for the spacecraft assessment mode,and the LEO region is selected for the spatial density assessment mode.The analysis indicates that compared with previous algorithms,the variable step-size orbital propagating algorithm based on semi-major axis control is more precise,the spatial density algorithm based on the second zonal harmonic of the non-spherical Earth gravity(J_(2))is more applicable,and the result of the position-centered spacecraft flux algorithm is more convergent.The comparison shows that SDEEM2019 and MASTER-8 have consistent trends due to similar modeling processes,while the differences between SDEEM2019 and ORDEM 3.1 are mainly caused by different modeling approaches for uncatalogued debris.展开更多
The generation of methylsufonyl-containing thioflavones through a radical relay reaction of methylthiolated phenylalkynones and potassium metabisulfite in the presence of a catalytic amount of sodium methylsulfinate a...The generation of methylsufonyl-containing thioflavones through a radical relay reaction of methylthiolated phenylalkynones and potassium metabisulfite in the presence of a catalytic amount of sodium methylsulfinate as an initiator under photoinduced conditions is developed.This photocatalytic reaction proceeds smoothly with good functional group tolerance under visible light irradiation.Preliminary mechanistic investigations indicate that this reaction occurs through a radical relay pathway.展开更多
文摘The impact sensitivity assessment of spacecraft is to obtain the probability of spacecraft encountering the OD/M(orbital debris or meteoroid),which is a prerequisite for survivability assessment of on-orbit spacecraft.An impact sensitivity assessment method of spacecraft based on virtual exterior wall was proposed to improve the computational efficiency.This method eliminates determination of the outermost surface elements of the spacecraft before generating the debris rays,which are assumed to originate from a non-concave virtual wall that completely wraps the spacecraft.The Dist Mesh method was adopted for the generating of the virtual wall to ensure its mesh quality.The influences of the sizes,mesh densities,shapes of the virtual wall on the efficiency and accuracy were considered to obtain the best combination of the size and mesh density of the wall and spacecraft.The results of this method were compared with those of S3DE(Survivability of Spacecraft in Space Debris Environment),BUMPER,MDPANTO,ESABASE2/Debris to verify the feasibility of the method.The PCHIP(Piecewise Cubic Hermite Interpolating Polynomial)was used to fit the size vs.flux relationship of the space debris to acquire the impact probability of OD/M with arbitrary size on the spacecraft.
基金supported by National Natural Science Foundation of China(21878062)
文摘The controllable construction of two-dimensional(2D)metal–organic framework(MOF)nanosheets with favorable electrochemical performances is greatly challenging for energy storage.Here,we design an in situ induced growth strategy to construct the ultrathin carboxylated carbon nanotubes(C-CNTs)interpenetrated nickel MOF(Ni-MOF/C-CNTs)nanosheets.The deliberate thickness and specific surface area of novel 2D hybrid nanosheets can be effectively tuned via finely controlling C-CNTs involvement.Due to the unique microstructure,the integrated 2D hybrid nanosheets are endowed with plentiful electroactive sites to promote the electrochemical performances greatly.The prepared Ni-MOF/C-CNTs nanosheets exhibit superior specific capacity of 680 C g^−1 at 1 A g^−1 and good capacity retention.The assembled hybrid device demonstrated the maximum energy density of 44.4 Wh kg^−1 at a power density of 440 W kg^−1.Our novel strategy to construct ultrathin 2D MOF with unique properties can be extended to synthesize various MOF-based functional materials for diverse applications.
基金the Career Development Program for Yong and Middle-aged Teachers in Shenyang Pharmaceutical University。
文摘Although the appearance of Doxil alleviated the cardiotoxicity of DOX, the progression-free survival of patients was not prolonged compared with traditional medication regimens, and side effects such as hand-foot syndrome has occurred. In order to solve this dilemma, we have designed a novel co-delivery strategy to construct a co-loaded liposome of berberine(BER) and doxorubicin(DOX), which was called Lipo Be Do. The optimal synergistic ratio of the two drugs was screened by cell cytotoxicity experiments in vitro, and the optimal attenuation ratio was further determined by in vivo cardiac H&E staining pathological sections. The optimal combination treatment caused a robust increase in apoptotic cells of 4T1, as compared to drug alone treatment. The prepared co-loaded liposome, Lipo Be Do, had high encapsulation efficiency and good stability. The nanoliposome carrier controlled the biological fate of the drugs and maintained a pre-defined optimal ratio in vivo. The Lipo Be Do significantly inhibited tumor growth in 4T1 murine mammary carcinoma model compared with Doxil(P < 0.05), and completely overcame the myocardial rupture toxicity caused by Doxil in mice. Our co-loaded liposome delivery platform technology provided a new direction for the clinical treatment of triple-negative breast cancer and the safe application of DOX.
基金supported by the National Natural Science Foundation of China(32100502)the Ministry of Agriculture of China(2016ZX08009003-006)Science&Technology Department of Yunnan Province(202102AE090039).
文摘Background:The pig is an economically important livestock species and is a widely applied large animal model in medical research.Enhancers are critical regulatory elements that have fundamental functions in evolution,development and disease.Genome-wide quantification of functional enhancers in the pig is needed.Results:We performed self-transcribing active regulatory region sequencing(STARR-seq)in the porcine kidney epithelial PK15 and testicular ST cell lines,and reliably identified 2576 functional enhancers.Most of these enhancers were located in repetitive sequences and were enriched within silent and lowly expressed genes.Enhancers poorly overlapped with chromatin accessibility regions and were highly enriched in chromatin with the repressive histone modification H3K9me3,which is different from predicted pig enhancers detected using ChIP-seq for H3K27ac or/and H3K4me1 modified histones.This suggests that most pig enhancers identified with STARR-seq are endogenously repressed at the chromatin level and may function during cell type-specific development or at specific developmental stages.Additionally,the PPP3CA gene is associated with the loin muscle area trait and the QKI gene is associated with alkaline phosphatase activity that may be regulated by distal functional enhancers.Conclusions:In summary,we generated the first functional enhancer map in PK15 and ST cells for the pig genome and highlight its potential roles in pig breeding.
文摘In this study, a new on-line health monitoring technology for the determination of the penetration of environment solution into epoxy resin was pursued. A corrosion sensor including plastic optical fiber and pH indicator was fabricated. The color-change layer of this sensor appeared after immersion in sulfuric acid solution, which could be examined by using optical fiber and spectrophotometer. The results showed that the penetration of sulfuric acid was detected by adding bromophenol blue （BPB） in the corrosion sensor. This system could be applied to on-line health monitoring of chemical equipment structures.
基金supported by the National Natural Science Foundation of China(32150023)。
文摘A common approach in therapeutic protein development involves employing synthetic ligands with multivalency,enabling sophisticated control of signal transduction.Leveraging the emerging concept of liquid-liquid phase separation(LLPS)and its ability to organize cell surface receptors into functional compartments,we herein have designed modular ligands with phase-separation modalities to engineer programmable interreceptor communications and precise control of signal pathways,thus inducing the rapid,potent,and specific apoptosis of tumor cells.Despite their simplicity,these“triggers”,named phase-separated Tumor Killers(hereafter referred to as psTK),are sufficient to yield interreceptor clustering of death receptors(represented by DR5)and tumor-associated receptors,with notable features:LLPSmediated robust high-order organization,well-choreographed conditional activation,and broad-spectrum capacity to potently induce apoptosis in tumor cells.The development of novel therapeutic proteins with phase-separation modalities showcases the power of spatially reorganizing signal transduction.This approach facilitates the diversification of cell fate and holds promising potential for targeted therapies against challenging tumors.
文摘As an essential tool for realistic description of the current or future debris environment,the Space Debris Environment Engineering Model(SDEEM)has been developed to provide support for risk assessment of spacecraft.In contrast with SDEEM2015,SDEEM2019,the latest version,extends the orbital range from the Low Earth Orbit(LEO)to Geosynchronous Orbit(GEO)for the years 1958-2050.In this paper,improved modeling algorithms used by SDEEM2019 in propagating simulation,spatial density distribution,and spacecraft flux evaluation are presented.The debris fluxes of SDEEM2019 are compared with those of three typical models,i.e.,SDEEM2015,Orbital Debris Engineering Model 3.1(ORDEM 3.1),and Meteoroid and Space Debris Terrestrial Environment Reference(MASTER-8),in terms of two assessment modes.Three orbital cases,including the Geostationary Transfer Orbit(GTO),Sun-Synchronous Orbit(SSO)and International Space Station(ISS)orbit,are selected for the spacecraft assessment mode,and the LEO region is selected for the spatial density assessment mode.The analysis indicates that compared with previous algorithms,the variable step-size orbital propagating algorithm based on semi-major axis control is more precise,the spatial density algorithm based on the second zonal harmonic of the non-spherical Earth gravity(J_(2))is more applicable,and the result of the position-centered spacecraft flux algorithm is more convergent.The comparison shows that SDEEM2019 and MASTER-8 have consistent trends due to similar modeling processes,while the differences between SDEEM2019 and ORDEM 3.1 are mainly caused by different modeling approaches for uncatalogued debris.
基金supported by the National Natural Science Foundation of China(21674030,22075061 and 51790502)the Funding of Key Laboratory of Bioinspired Materials and Interfacial Science,the Technical Institute of Physics and Chemistry,Chinese Academy of Sciences,China National Postdoctoral Program for Innovative Talents(BX20200106)。
基金Financial support from the National Natural Science Foundation of China(No.21901178)the Natural Science Foundation of Zhejiang Province(LY21B020002)+1 种基金the Leading Innovative and Entrepreneur Team Introduction Program of Zhejiang(No.2019R01005)the Open Research Fund of School of Chemistry and Chemical Engineering,Henan Normal University(2020ZD04)is gratefully acknowledged.
文摘The generation of methylsufonyl-containing thioflavones through a radical relay reaction of methylthiolated phenylalkynones and potassium metabisulfite in the presence of a catalytic amount of sodium methylsulfinate as an initiator under photoinduced conditions is developed.This photocatalytic reaction proceeds smoothly with good functional group tolerance under visible light irradiation.Preliminary mechanistic investigations indicate that this reaction occurs through a radical relay pathway.
