Infectious diseases severely threaten public health and global biosafety.In addition to transmission through the air,pathogenic microorganisms have also been detected in environmental liquid samples,such as sewage wat...Infectious diseases severely threaten public health and global biosafety.In addition to transmission through the air,pathogenic microorganisms have also been detected in environmental liquid samples,such as sewage water.Conventional biochemical detection methodologies are time-consuming and cost-ineffective,and their detection limits hinder early diagnosis.In the present study,ultrafine plasmonic fiber probes with a diameter of 125μm are fabricated for clustered regularly interspaced short palindromic repeats/CRISPR-associated protein(CRISPR/Cas)-12a-mediated sensing of severe acute respiratory syndrome coronavirus 2(SARS-CoV-2).Single-stranded DNA exposed on the fiber surface is trans-cleaved by the Cas12a enzyme to release gold nanoparticles that are immobilized onto the fiber surface,causing a sharp reduction in the surface plasmon resonance(SPR)wavelength.The proposed fiber probe is virus-specific with the limit of detection of~2,300 copies/ml,and genomic copy numbers can be reflected as shifts in wavelengths.A total of 21 sewage water samples have been examined,and the data obtained are consistent with those of quantitative polymerase chain reaction(qPCR).In addition,the Omicron variant and its mutation sites have been fast detected using S gene-specific Cas12a.This study provides an accurate and convenient approach for the real-time surveillance of microbial contamination in sewage water.展开更多
Purpose–This study aims to investigate the safety and liability of autonomous vehicles(AVs),and identify the contributing factors quantitatively so as to provide potential insights on safety and liability of AVs.Desi...Purpose–This study aims to investigate the safety and liability of autonomous vehicles(AVs),and identify the contributing factors quantitatively so as to provide potential insights on safety and liability of AVs.Design/methodology/approach–The actual crash data were obtained from California DMV and Sohu websites involved in collisions of AVs from 2015 to 2021 with 210 observations.The Bayesian random parameter ordered probit model was proposed to reflect the safety and liability of AVs,respectively,as well as accommodating the heterogeneity issue simultaneously.Findings–The findings show that day,location and crash type were significant factors of injury severity while location and crash reason were significant influencing the liability.Originality/value–The results provide meaningful countermeasures to support the policymakers or practitioners making strategies or regulations about AV safety and liability.展开更多
基金supported by the National Natural Science Foundation of China(no.U1813207)the State Key Research Development Program of China(no.YS2022YFB3200011)+4 种基金Stabilization Support Program for Higher Education Institutions of Shenzhen(no.20200812115548001)Shenzhen Bay Laboratory Open Fund Project(no.SZBL2021080601012)High-end Talent Scientific Research Startup Project(no.827-000636)Shenzhen Science and Technology R&D and Innovation Foundation(no.JCJY20200109105608771)The authors acknowledge the support and funding of King Khalid University through Research Center for Advanced Materials Science(RCAMS)under grant no.RCAMS/KKU/0010/21.
文摘Infectious diseases severely threaten public health and global biosafety.In addition to transmission through the air,pathogenic microorganisms have also been detected in environmental liquid samples,such as sewage water.Conventional biochemical detection methodologies are time-consuming and cost-ineffective,and their detection limits hinder early diagnosis.In the present study,ultrafine plasmonic fiber probes with a diameter of 125μm are fabricated for clustered regularly interspaced short palindromic repeats/CRISPR-associated protein(CRISPR/Cas)-12a-mediated sensing of severe acute respiratory syndrome coronavirus 2(SARS-CoV-2).Single-stranded DNA exposed on the fiber surface is trans-cleaved by the Cas12a enzyme to release gold nanoparticles that are immobilized onto the fiber surface,causing a sharp reduction in the surface plasmon resonance(SPR)wavelength.The proposed fiber probe is virus-specific with the limit of detection of~2,300 copies/ml,and genomic copy numbers can be reflected as shifts in wavelengths.A total of 21 sewage water samples have been examined,and the data obtained are consistent with those of quantitative polymerase chain reaction(qPCR).In addition,the Omicron variant and its mutation sites have been fast detected using S gene-specific Cas12a.This study provides an accurate and convenient approach for the real-time surveillance of microbial contamination in sewage water.
基金National Natural Science Foundation of China(No.52072214)the project of Tsinghua University-Toyota Joint Research Center for AI technology of Automated Vehicle(No.TTAD2021-10).
文摘Purpose–This study aims to investigate the safety and liability of autonomous vehicles(AVs),and identify the contributing factors quantitatively so as to provide potential insights on safety and liability of AVs.Design/methodology/approach–The actual crash data were obtained from California DMV and Sohu websites involved in collisions of AVs from 2015 to 2021 with 210 observations.The Bayesian random parameter ordered probit model was proposed to reflect the safety and liability of AVs,respectively,as well as accommodating the heterogeneity issue simultaneously.Findings–The findings show that day,location and crash type were significant factors of injury severity while location and crash reason were significant influencing the liability.Originality/value–The results provide meaningful countermeasures to support the policymakers or practitioners making strategies or regulations about AV safety and liability.
