With adjustable amphiphilicity and anionic/cationic charge,biodegradability and biocompatibility,amino acid-based poly(ester amide)s(PEAs)have drawn attention in the research of tissue engineered vascular grafts.In th...With adjustable amphiphilicity and anionic/cationic charge,biodegradability and biocompatibility,amino acid-based poly(ester amide)s(PEAs)have drawn attention in the research of tissue engineered vascular grafts.In this work,L-phenylalanine-based PEAs with or without L-lysine were synthesized through polycondensation,and ultrafine fibrous grafts consisted of PEAs and poly(ε-caprolactone)(PCL)in given mass ratios were further prepared via blend electrospinning.Surface characterizations by Fourier transform infrared spectroscopy and X-ray photoelectron spectroscopy confirmed the chemical structure,and the wettability indicated that the prepared PCL/PEAs electrospun membranes exhibited less hydrophobic than PCL.Tensile results showed that the PCL/PEAs membranes possessed suitable mechanical properties,which could meet the requirements of artificial blood vessels.Cell culture and hemolytic tests exhibited that the PCL/PEAs electrospun membranes are biocompatible.In general,the electrospun grafts of PCL/PEAs could be applied for vascular repair.展开更多
Mismatched biomechanical properties between artificial vascular grafts and native blood vessels can result in intimal hyperplasia,especially for the implantation of small-diameter blood vessels.Ideal biomaterials for ...Mismatched biomechanical properties between artificial vascular grafts and native blood vessels can result in intimal hyperplasia,especially for the implantation of small-diameter blood vessels.Ideal biomaterials for vascular repair still remain challenged.Biodegradable poly(ε-caprolactone)(PCL)has been applied for the preparation of electrospun vascular grafts,but more efforts are needed to improve its compliance with tissue growth.Herein,L-arginine-based poly(ester urethane)urea(PEUU)with both elasticity and biodegradability was synthesized so as to enhance the biomechanical properties of vascular grafts by blending electrospinning with PCL in a given mass ratio.It was exhibited that the prepared electrospun PCL/PEUU fibrous membranes were suitable for cell proliferation with normal cell morphology.More importantly,the electrospun membrane with 1/1 mass ratio of PCL/PEUU(PEUU50)showed specific flexibility,exhibiting more suitable mechanical properties matching to the native blood vessels.Specifically,the PEUU50 electrospun membrane demonstrated significantly lower Young’s modulus(9.3±0.8 MPa)and tensile strength(6.0±0.5 MPa),and extremely higher elongation(389%±24%)in wet state than those(16.3±3.0 MPa,11.4±7.1 MPa and 196%±57%,respectively)of the pristine PCL membrane.Overall,this study demonstrated the great potential of amino acid-based PEUUs for the application in small-diameter vascular grafts.展开更多
Cardiovascular diseases have been the leading cause of morbidity and mortality in the world recently.With the growing aging population accompanied by chronic diseases,such as uremia and diabetes,there is an increasing...Cardiovascular diseases have been the leading cause of morbidity and mortality in the world recently.With the growing aging population accompanied by chronic diseases,such as uremia and diabetes,there is an increasing clinical demand for vascular grafts with proper performance.Although some achievements have been made in the development of tissue-engineered vascular grafts composed of natural and synthetic polymeric materials or decellularized vessels,clinical applications with a diameter of less than 6 mm are still principally derived from autografts,such as autologous saphenous veins.Many challenges remain in antithrombosis,rapid endothelialization,modulating the inflammatory response and inhibition of intimal hyperplasia and calcification.In the review,recent progress in the electrospinning of biodegradable polymers for vascular regeneration are summarized,especially from the view of biomechanical factors.Hybrid vascular grafts consisting of natural and synthetic polymers with multicomponent,di-or tri-layers are focused in order to provide novel experiences in biomaterials for applications in this field.展开更多
基金supported by the National Natural Science Foundation of China(No.52073204).
文摘With adjustable amphiphilicity and anionic/cationic charge,biodegradability and biocompatibility,amino acid-based poly(ester amide)s(PEAs)have drawn attention in the research of tissue engineered vascular grafts.In this work,L-phenylalanine-based PEAs with or without L-lysine were synthesized through polycondensation,and ultrafine fibrous grafts consisted of PEAs and poly(ε-caprolactone)(PCL)in given mass ratios were further prepared via blend electrospinning.Surface characterizations by Fourier transform infrared spectroscopy and X-ray photoelectron spectroscopy confirmed the chemical structure,and the wettability indicated that the prepared PCL/PEAs electrospun membranes exhibited less hydrophobic than PCL.Tensile results showed that the PCL/PEAs membranes possessed suitable mechanical properties,which could meet the requirements of artificial blood vessels.Cell culture and hemolytic tests exhibited that the PCL/PEAs electrospun membranes are biocompatible.In general,the electrospun grafts of PCL/PEAs could be applied for vascular repair.
基金supported by the National Natural Science Foundation of China(Grant No.52073204)。
文摘Mismatched biomechanical properties between artificial vascular grafts and native blood vessels can result in intimal hyperplasia,especially for the implantation of small-diameter blood vessels.Ideal biomaterials for vascular repair still remain challenged.Biodegradable poly(ε-caprolactone)(PCL)has been applied for the preparation of electrospun vascular grafts,but more efforts are needed to improve its compliance with tissue growth.Herein,L-arginine-based poly(ester urethane)urea(PEUU)with both elasticity and biodegradability was synthesized so as to enhance the biomechanical properties of vascular grafts by blending electrospinning with PCL in a given mass ratio.It was exhibited that the prepared electrospun PCL/PEUU fibrous membranes were suitable for cell proliferation with normal cell morphology.More importantly,the electrospun membrane with 1/1 mass ratio of PCL/PEUU(PEUU50)showed specific flexibility,exhibiting more suitable mechanical properties matching to the native blood vessels.Specifically,the PEUU50 electrospun membrane demonstrated significantly lower Young’s modulus(9.3±0.8 MPa)and tensile strength(6.0±0.5 MPa),and extremely higher elongation(389%±24%)in wet state than those(16.3±3.0 MPa,11.4±7.1 MPa and 196%±57%,respectively)of the pristine PCL membrane.Overall,this study demonstrated the great potential of amino acid-based PEUUs for the application in small-diameter vascular grafts.
基金This work was supported by the National Natural Science Foundation of China(No.52073204).
文摘Cardiovascular diseases have been the leading cause of morbidity and mortality in the world recently.With the growing aging population accompanied by chronic diseases,such as uremia and diabetes,there is an increasing clinical demand for vascular grafts with proper performance.Although some achievements have been made in the development of tissue-engineered vascular grafts composed of natural and synthetic polymeric materials or decellularized vessels,clinical applications with a diameter of less than 6 mm are still principally derived from autografts,such as autologous saphenous veins.Many challenges remain in antithrombosis,rapid endothelialization,modulating the inflammatory response and inhibition of intimal hyperplasia and calcification.In the review,recent progress in the electrospinning of biodegradable polymers for vascular regeneration are summarized,especially from the view of biomechanical factors.Hybrid vascular grafts consisting of natural and synthetic polymers with multicomponent,di-or tri-layers are focused in order to provide novel experiences in biomaterials for applications in this field.
