矮壮素对黄芪13种有效成分含量的影响研究

目的 分析植物生长调节剂矮壮素对黄芪有效成分含量的影响,为黄芪规范化生产提供依据。方法 采用田间小区试验,于黄芪生长期喷施不同浓度的矮壮素,建立黄芪的LC-MS/MS方法并测定其根中13种活性成分的含量。结果 喷施矮壮素后,黄芪根中黄芪皂苷I、黄芪甲苷、异黄芪皂苷Ⅱ、异黄芪皂苷Ⅳ和芒柄花黄素含量显著提高,其中芒柄花黄素含量增加最多(70.08%),而美迪紫檀苷、黄芪紫檀烷苷、毛蕊异黄酮苷含量具有显著性降低,其中黄芪紫檀烷苷含量降低最多(47.78%)。结论 施用矮壮素可引起黄芪多个活性成分含量变化,且不同成分变化无规律,总体改变了多个成分间的比例。

威灵仙一种新伪品的生药学和分子生药学鉴别

目的：运用生药学和分子生药学研究思路和方法,对市场上一种威灵仙新伪品进行鉴别,为保障威灵仙药材的安全使用提供实验依据。方法：对威灵仙及其伪品进行性状、显微等生药学研究,描述威灵仙及其伪品的生药学鉴别特征。对威灵仙及其伪品的分子生药学研究,通过试剂盒提取样品的DNA,聚合酶链式反应（PCR）扩增及双向测序获得其内转录间隔区（ITS）序列,根据美国国家生物技术信息中心（NCBI）数据库中已收录的ITS序列进行相似度分析及DNA条形码溯源。结果：威灵仙及伪品的生药学鉴别特征主要有外皮颜色以及有无茎节等;横切面显微特征区别主要为有无髓。分子生药学研究表明,该伪品的基原植物为紫金牛科紫金牛Ardisia japonica。结论：该威灵仙伪品为紫金牛的茎及根茎,该伪品与威灵仙在成分、功能主治方面差别较大,在临床应用上应严格区分。

Sources of varieties and quality of circular Fructus Ligustri Lucidi

This study aimed to trace sources and quantitatively analyze the specnuezhenide content of circular Fructus Ligustri Lucidi for clinical use. Different specifications of Fructus Ligustri Lucidi were identified using DNA barcoding technology and the specnuezhenide content was analyzed by High Performance Liquid Chromatography(HPLC). The ITS sequence of circular Fructus Ligustri Lucidi was identical to that of standard privet, which was determined through botanical identification. ITS sequence similarity between circular Fructus Ligustri Lucidi and Fructus Ligustri Lucidi which was registered in NCBI ranged from 99.5% to 100%. The sequences of circular and other Fructus Ligustri Lucidi were clustered in a Neighbor-Joining tree with bootstrap value of 95, and these sequences could be distinguished from adulterants. Conforming to pharmacopoeia standard, the average specnuezhenide content of circular Fructus Ligustri Lucidi was higher than that of chicken waist Fructus Ligustri Lucidi. Circular Fructus Ligustri Lucidi derived from Ligustrum lucidum Ait. and the specnuezhenide content was higher in circular Fructus Ligustri Lucidi than that in chicken waist Fructus Ligustri Lucidi.