目的基于文献计量学探究近20年国内外关于视网膜色素变性(RP)的研究现状和热点前沿。方法分别检索中国知网数据库(CNKI)和Web of Science核心数据库(WoSCC)有关RP实验研究和临床研究的文献,利用Citespace进行可视化分析。结果共筛选出2...目的基于文献计量学探究近20年国内外关于视网膜色素变性(RP)的研究现状和热点前沿。方法分别检索中国知网数据库(CNKI)和Web of Science核心数据库(WoSCC)有关RP实验研究和临床研究的文献,利用Citespace进行可视化分析。结果共筛选出2277篇文献,CNKI和WoSCC中分别检索出354篇中文文献和1923篇英文文献,年度发文量总体呈上升趋势。湖南中医药大学第一附属医院和伦敦大学分别是CNKI和WoSCC中发文量最大的机构。彭清华和彭俊是CNKI中发文量最大的作者,Tsang Stephen H是WoSCC中发文量最大的作者。由关键词分析可知WoSCC文献的研究重点是遗传学和基因组学研究,而CNKI文献的研究重点是中医药治疗。结论本研究利用Citespace软件对RP研究领域的现状进行分析,明确当前的研究热点和前沿,为关注该领域的研究者提供了新思路和理论参考。展开更多
Objective To explore the molecular targets and associated potential pathways of Lycii Fructus(LF,Gou Qi Zi,枸杞子)in the treatment of retinitis pigmentosa(RP)by the approaches of network pharmacology and bioinformatic...Objective To explore the molecular targets and associated potential pathways of Lycii Fructus(LF,Gou Qi Zi,枸杞子)in the treatment of retinitis pigmentosa(RP)by the approaches of network pharmacology and bioinformatics.Methods The potential blood-entry active ingredients and targets of LF were retrieved by Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform(TCMSP).RP-related gene targets were retrieved through disease comprehensive databases.Protein-protein interaction(PPI)network of LF component-targets and RP disease-targets was constructed by STRING,and the intersection of the 2 networks was extracted.Gene Ontology and Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway analysis of theintersection network were conducted by Database for Annotation,Visualization and Integrated Discovery(DAVID).CytoHubba was used to screen the key targets.Results A total of 188 chemical constituents related to LF was retrieved from TCMSP database.45 active ingredients were screened according to pharmacokinetic parameters oral bioavailability(OB)and drug similarity(DL).36 active ingredients were further screened and 201 targets related to these constituents were obtained.206 target genes directly related to RP were obtained from the disease comprehensive databases,and 89 genes were obtained from the intersection of componenttarget and disease-target PPI network.These genes were mainly involved in intracellular signal transduction,GTPase activity regulation,cell morphology regulation,and other biological processes.Molecular functions were mainly related to Rho guanine nucleotide exchange factor activity,GTPase activator activity,receptor signal protein serine/threonine kinase activity and so on.They were enriched in the cytoplasm,cell membrane,Golgi apparatus,and other regions.The mechanism was related to cell cycle pathways,neurotrophin signaling pathways,Ras signaling pathways,and so on.10 key gene targets of LF in the treatment of RP were screened.Conclusions The material basis for LF to exert its pharmacodynamic effect is 36 active ingredients such as cycloartenol,mandenol,and so on.The key targets of LF in the treatment of RP include 10 genes,such as Rho,PAK,and so on.The main mechanism is related to the regulation of the Ras signaling pathway,neurotrophin signaling pathway,cell cycle related pathway,and other signaling networks.展开更多
文摘目的基于文献计量学探究近20年国内外关于视网膜色素变性(RP)的研究现状和热点前沿。方法分别检索中国知网数据库(CNKI)和Web of Science核心数据库(WoSCC)有关RP实验研究和临床研究的文献,利用Citespace进行可视化分析。结果共筛选出2277篇文献,CNKI和WoSCC中分别检索出354篇中文文献和1923篇英文文献,年度发文量总体呈上升趋势。湖南中医药大学第一附属医院和伦敦大学分别是CNKI和WoSCC中发文量最大的机构。彭清华和彭俊是CNKI中发文量最大的作者,Tsang Stephen H是WoSCC中发文量最大的作者。由关键词分析可知WoSCC文献的研究重点是遗传学和基因组学研究,而CNKI文献的研究重点是中医药治疗。结论本研究利用Citespace软件对RP研究领域的现状进行分析,明确当前的研究热点和前沿,为关注该领域的研究者提供了新思路和理论参考。
基金funding support from the National Natural Science Foundation of China (No. 81804150 and No. 81703920)Project funded by China Postdoctoral Science Foundation (No. 2019M662790)+4 种基金Natural Science Foundation of Hunan Province, China (No. 2019JJ50442 and No. 2019JJ40226)Research-based Learning and Innovative Experiment Program Project for Hunan University Students (No. 2017280)Scientific Research Project of Hunan Traditional Chinese Medicine Administration (No. 201780)Open Fund Project of Hunan Provincial Key Laboratory for Prevention and Treatment of Ophthalmology and Otolaryngology Diseases with Chinese Medicine (No. 2018YZD05)Open Fund of the Domestic First-class Discipline Construction Project of Chinese Medicine of Hunan University of Chinese Medicine (No. 2018ZYX20 and No. 2018ZYX26)
文摘Objective To explore the molecular targets and associated potential pathways of Lycii Fructus(LF,Gou Qi Zi,枸杞子)in the treatment of retinitis pigmentosa(RP)by the approaches of network pharmacology and bioinformatics.Methods The potential blood-entry active ingredients and targets of LF were retrieved by Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform(TCMSP).RP-related gene targets were retrieved through disease comprehensive databases.Protein-protein interaction(PPI)network of LF component-targets and RP disease-targets was constructed by STRING,and the intersection of the 2 networks was extracted.Gene Ontology and Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway analysis of theintersection network were conducted by Database for Annotation,Visualization and Integrated Discovery(DAVID).CytoHubba was used to screen the key targets.Results A total of 188 chemical constituents related to LF was retrieved from TCMSP database.45 active ingredients were screened according to pharmacokinetic parameters oral bioavailability(OB)and drug similarity(DL).36 active ingredients were further screened and 201 targets related to these constituents were obtained.206 target genes directly related to RP were obtained from the disease comprehensive databases,and 89 genes were obtained from the intersection of componenttarget and disease-target PPI network.These genes were mainly involved in intracellular signal transduction,GTPase activity regulation,cell morphology regulation,and other biological processes.Molecular functions were mainly related to Rho guanine nucleotide exchange factor activity,GTPase activator activity,receptor signal protein serine/threonine kinase activity and so on.They were enriched in the cytoplasm,cell membrane,Golgi apparatus,and other regions.The mechanism was related to cell cycle pathways,neurotrophin signaling pathways,Ras signaling pathways,and so on.10 key gene targets of LF in the treatment of RP were screened.Conclusions The material basis for LF to exert its pharmacodynamic effect is 36 active ingredients such as cycloartenol,mandenol,and so on.The key targets of LF in the treatment of RP include 10 genes,such as Rho,PAK,and so on.The main mechanism is related to the regulation of the Ras signaling pathway,neurotrophin signaling pathway,cell cycle related pathway,and other signaling networks.
