灵龟八法针刺疗法治疗失眠的Meta分析

目的:评价灵龟八法针刺疗法治疗失眠的疗效和安全性,以更好服务临床,为失眠的治疗提供参考思路。方法:检索CNKI,CBM,万方,维普,中国临床试验注册中心数据库中使用灵龟八法对比其他干预方案治疗失眠的文献,检索期限为建库至2021年8月1日,由2名研究员对文献进行独立的质量评价和效应量提取,使用STATA16.0和Review5.3对文献进行Meta分析和质量评价。通过筛选,共有18篇符合条件的文献参与Meta分析,共1583例病例。结果:(1)灵龟八法治疗失眠疗效的有效率较对照组高[RR=1.213,95%CI(1.155,1.274),Z=7.71,P<0.01];(2)灵龟八法治疗失眠的匹兹堡睡眠质量指数(PSQI)较其他针刺疗法治疗后评分更低[WMD=−2.33,95%CI(−2.75,−1.91),Z=10.97,P<0.01];(3)灵龟八法治疗失眠治疗后随访1个月PSQI评分较对照组治疗后评分更低[WMD=−3.263,95%CI(−3.446,−3.080),Z=34.96,P<0.01];(4)没有研究报告在治疗组和对照组治疗中发生严重不良反应。结论:基于目前研究,灵龟八法治疗失眠效果优于对照组,适合临床推广应用,但仍需更多高质量的临床研究证明其疗效可靠。

A Meta-analysis of the spirit turtle eight method Acupuncture Therapy for Insomnia

Objective:To systematically evaluate the efficacy and safety of the spirit turtle eight method acupuncture therapy in the treatment of insomnia,to better serve the clinic and provide reference ideas for the treatment of insomnia.Methods:Search CNKI,CBM,Wanfang,Weipu,China Clinical Trial Registry databases on the use of the spirit turtle eight method compared with others interventions to treat insomnia.The search period is from the library until August 1,2021,two researchers will conduct independent quality evaluation and effect size extraction on the literature,and use STATA16.0 and Review5.3 to conduct Meta analysis and quality evaluation on the literature.Through screening,a total of 18 eligible documents participated in the meta-analysis,with a total of 1583 cases.Results:①The effective rate of the spirit turtle eight method in the treatment of insomnia is higher than the control group[RR=1.213,95%CI(1.155,1.274),Z=7.71,P<0.01];②The Pittsburgh Sleep Quality Index(PSQI)score is lower than other acupuncture treatments[WMD=-2.33,95%CI(-2.75,-1.91),Z=10.97,P<0.01];③The one-month follow-up PSQI score after the treatment of insomnia by the spirit turtle eight method is lower than the control group[WMD=-3.263,95%CI(-3.446,-3.080),Z=34.96,P<0.01];④No studies have reported serious adverse reactions in the treatment group and control group.Conclusion:Based on the current research,the spirit turtle eight method are better than the control group in the treatment of insomnia,and are suitable for clinical application.However,more high-quality clinical studies are still needed to prove its efficacy.

灵龟八法按时开穴灸对衰老大鼠睾丸细胞凋亡的影响

目的:观察灵龟八法按时开穴灸对衰老大鼠超氧化物歧化酶(SOD)、丙二醛(MDA)含量及抗凋亡调控因子B淋巴细胞瘤2(Bcl-2)和促凋亡调控因子Bcl相关X蛋白(Bax)表达的影响,探讨其作用机制。方法:SD大鼠随机分为空白组、模型组、灵龟八法组,每组8只。除空白组外,其余两组均以D-半乳糖溶液(500 mg/kg)腹腔注射复制亚急性衰老模型。造模成功后,灵龟八法组大鼠予以灵龟八法按时开穴灸治疗,每穴艾灸3壮,1次/d,连续28 d。采用ELISA法观察大鼠血清中SOD、MDA的含量;HE染色法观察大鼠睾丸组织一般形态和间质细胞数量的变化;TUNEL染色法检测大鼠睾丸细胞凋亡率;Western blot法检测大鼠睾丸组织中Bcl-2、Bax蛋白的表达水平。结果:与空白组比较,模型组大鼠血清SOD含量、睾丸组织间质细胞数量减少(P<0.01),血清MDA含量增多(P<0.01),睾丸细胞凋亡率、睾丸组织中Bax蛋白的相对表达量升高(P<0.01),睾丸组织中Bcl-2蛋白的相对表达量降低(P<0.01)。与模型组比较,灵龟八法组大鼠血清SOD含量、睾丸组织间质细胞数量增多(P<0.01),血清MDA含量减少(P<0.01),睾丸细胞凋亡率、睾丸组织中Bax蛋白的相对表达量降低(P<0.01),睾丸组织中Bcl-2蛋白的相对表达量升高(P<0.01)。结论:灵龟八法按时开穴灸能够起到延缓大鼠睾丸衰老的作用,其机制可能与平衡自由基的代谢,减少机体氧化损伤,进而抑制睾丸细胞凋亡有关。