Because of limited viral replication and lack of cytopathic effect in cell culture,a new PCR-based rapid seroneutralization assay for detection of GII.4norovirus neutralized antibodies was developed with serum samples...Because of limited viral replication and lack of cytopathic effect in cell culture,a new PCR-based rapid seroneutralization assay for detection of GII.4norovirus neutralized antibodies was developed with serum samples from acute-phase patients,convalescent-phase patients and healthy controls.According to this study,neutralizing antibodies were detected in 100% ofconvalescent-phase sera, and in 2.5% of healthy controls sera. However, all of the acute-phase serum samples could not neutralize virus efficiently. Compared to the results from ELISA （96.2% at sensitivity and 80% at specificity）, the present in vitro neutralization assay is more specific and more sensitive.展开更多
文摘Because of limited viral replication and lack of cytopathic effect in cell culture,a new PCR-based rapid seroneutralization assay for detection of GII.4norovirus neutralized antibodies was developed with serum samples from acute-phase patients,convalescent-phase patients and healthy controls.According to this study,neutralizing antibodies were detected in 100% ofconvalescent-phase sera, and in 2.5% of healthy controls sera. However, all of the acute-phase serum samples could not neutralize virus efficiently. Compared to the results from ELISA （96.2% at sensitivity and 80% at specificity）, the present in vitro neutralization assay is more specific and more sensitive.
