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MDS-RA/RARS/RCMD患者外周血循环CD34^+细胞计数的临床价值 被引量:1
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作者 董海波 曾慧 +3 位作者 张启国 周敏 袁翠英 陈兵 《检验医学》 CAS 2018年第5期388-392,共5页
目的探讨骨髓增生异常综合征(MDS)-难治性贫血(RA)/难治性贫血伴环形铁粒幼细胞增多(RARS)/难治性贫血伴多系异常(RCMD)患者外周血循环CD34^+细胞计数的临床价值。方法采用流式细胞仪检测19名造血干细胞供者(正常对照者)、52例MDS-RA/RA... 目的探讨骨髓增生异常综合征(MDS)-难治性贫血(RA)/难治性贫血伴环形铁粒幼细胞增多(RARS)/难治性贫血伴多系异常(RCMD)患者外周血循环CD34^+细胞计数的临床价值。方法采用流式细胞仪检测19名造血干细胞供者(正常对照者)、52例MDS-RA/RARS/RCMD患者外周血循环CD34^+细胞百分比和计数。根据国际预后积分系统(IPSS)及世界卫生组织(WHO)分型预后积分系统(WPSS),将MDSRA/RARS/RCMD患者分为低危、中危Ⅰ、中危Ⅱ、高危及极低危、低危、中危、高危、极高危。评价外周血循环CD34^+细胞计数与MDS-RA/RARS/RCMD患者临床特点的相关性。结果 MDS-RA/RARS/RCMD患者外周血循环CD34^+细胞百分比和计数均高于正常对照者(P<0.01),外周血循环CD34^+细胞计数与患者性别、年龄、外周血血细胞减少程度、白细胞计数下降、幼稚前体细胞异常定位(ALIP)现象均无相关性(r<0.625,P>0.05),而与染色体核型异常、IPSS预后积分、WPSS预后积分、骨髓纤维化相关(r>0.995,P<0.01)。外周血循环CD34^+细胞计数>10.00×10~6/L的16例MDS-RA/RARS/RCMD患者中,有12例伴有复杂染色体核型异常,其中10例伴有7号染色体核型异常;有12例IPSS预后积分为中危Ⅱ;有13例WPSS预后积分为高危;9例伴有骨髓纤维化。36例外周血循环CD34^+细胞计数<10.00×10~6/L的患者均不伴有复杂染色体核型异常及7号染色体核型异常;所有患者IPSS预后积分均为低危或中危Ⅰ;除1例患者WPSS预后积分为高危外,其余患者的WPSS预后积分均为极低危、低危或中危;除1例患者伴有骨髓纤维化外,其余患者均不伴有骨髓纤维化。结论MDS-RA/RARS/RCMD患者外周血循环CD34^+细胞计数可出现异常增加,外周血循环CD34^+细胞计数检测有助于对MDS患者疾病危险度的进一步分层。 展开更多
关键词 循环CD34^+细胞 外周血 骨髓增生异常综合征
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PURIFICATION OF FLAVONES OF GINKGO LEAVES BY ADSORPTION HZ-841 RESIN 被引量:1
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作者 zenghui ZHOUXiaohua LIDuanhua 《Chinese Journal of Reactive Polymers》 2004年第1期83-88,共6页
The purification process of total flavones of ginkgo leaves by resin HZ-841 from ethanol extract was studied. First, the total flavone was extracted from the defatted powder of ginkgo L. biloba leaves. Effects of solv... The purification process of total flavones of ginkgo leaves by resin HZ-841 from ethanol extract was studied. First, the total flavone was extracted from the defatted powder of ginkgo L. biloba leaves. Effects of solvents and operation conditions were examined to get a relative high yield and purity in this step. The crude extract was further purified by resin HZ-841. Both adsorption and elution process were studied to obtain an optimized conditions, i.e., pH, flow rate, concentration. A yellow powder was obtained, of which 37.3% was flavones, obviously higher than the basic international standard of 24%. 展开更多
关键词 Total flavonoids Adsorption resin Purification.
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