目的系统评估孕前体质量指数与后代先天性心脏病的关联。方法系统检索PubMed、EMbase、Web of Scicence、万方、维普和中国知网,检索时间为建库至2017年12月,按纳入和排除标准筛选文献。采用NOS量表评估文献质量。结果共16篇研究纳入本M...目的系统评估孕前体质量指数与后代先天性心脏病的关联。方法系统检索PubMed、EMbase、Web of Scicence、万方、维普和中国知网,检索时间为建库至2017年12月,按纳入和排除标准筛选文献。采用NOS量表评估文献质量。结果共16篇研究纳入本Meta分析,其中病例对照研究13篇,队列研究3篇。低体质量(OR:1.03,95%CI:0.97~1.10)和超重(OR:1.05,95%CI:0.98~1.14)与后代先天性心脏病的发生风险无关,肥胖(OR:1.37,95%CI:1.17~1.61)可增加后代先天性心脏病的发生风险。剂量反应的Meta分析提示两者间存在非线性剂量反应关系,即随着孕前体质量指数的增加,后代发生先天性心脏病的风险也随之增加。结论肥胖影响后代先天性心脏病的发生风险,孕前体质量指数越大,后代发生先天性心脏病的风险越高。展开更多
In December 2019, Wuhan, the capital of Hubei Province in China,reported a series of unexplained cases of pneumonia, which has been confirmed to be an acute infectious respiratory disease caused by Novel Corona Virus ...In December 2019, Wuhan, the capital of Hubei Province in China,reported a series of unexplained cases of pneumonia, which has been confirmed to be an acute infectious respiratory disease caused by Novel Corona Virus 2019(2019-nCoV)infection. The World Health Organization(WHO) named this pneumonia Corona Virus Disease 2019(COVID-19). The 21 st century had witnessed the spread of two previously unrecognized coronaviruses around the world with high pathogenicity before, namely severe acute respiratory syndrome coronavirus(SARS-CoV) and Middle East respiratory syndrome coronavirus(MERS-CoV) respectively. At present, 2019-nCoV ’s genome has been sequenced by researchers all over the world represented by China. Based on the systematic review and analysis of the genomic sequence characteristics of three highly pathogenic coronaviruses, this paper expounds the basic characteristics of the genomes of SARS-CoV, MERS-CoV and 2019-nCoV,which all belong to β-coronaviruses, the functionally important ORFs, ORF1 a, ORF1 b and major structural proteins including the spike(S), membrane(M) and envelop(E) and nucleiccapsid(N) proteins have no significant difference with each other. However, as the majority of genetic variations being seen at the N-terminal part of S protein, 2019-nCoV aligned best with the bat SARS-like virus bat-SL-CoVZX45. And Structure analysis of the spike(S) protein of2019-nCoV showed that it’s S protein only binds weakly to the Angiotensin Converting Enzyme2(ACE2) receptor on human cells whereas the human SARS-Cov exhibits high affinity to the ACE receptor and MERS-Cov does not need ACE2 to infect human cells,its receptor on human cells is DPP4. In the future, we hope to provide new ideas for the prevention and treatment of coronavirus by strengthening the research of coronavirus S protein and ACE2 receptor, and continuous surveillance and genomic characterization of coronaviruses from bats are necessary due to potential risks of human infection induced by a genetic mutation.展开更多
This study aims to investigate the association of SNPs with tumor-associated latent loci. By analyzing the effect of gene mutations on the structure and function of proteins with the database, to speculate the destruc...This study aims to investigate the association of SNPs with tumor-associated latent loci. By analyzing the effect of gene mutations on the structure and function of proteins with the database, to speculate the destructive mutants. It was found that individuals with the genotype of the five key SNP loci were more susceptible to tumors; these specific markers can be used as a molecular marker to determine the susceptibility of a tumor to the individual, and to diagnose the population with high risk of tumor; and rs201154887 can be used as a new disease molecular marker.展开更多
Objective: To investigate the relationship between gene mutation and pathological type of lung cancer, inspect and verify the consistency between homologous genes mutation in various pathologic type. Methods: Combined...Objective: To investigate the relationship between gene mutation and pathological type of lung cancer, inspect and verify the consistency between homologous genes mutation in various pathologic type. Methods: Combined with the COSMIC and Uni Prot database, we obtained the reported overall big-sample mutation data of lung cancer and the protein sequences of the top 20 mutated genes,respectively. Analyze the data and cluster the protein sequences and then deduce the homologous gene. Ultimately, analyze the mutations of different pathological types of homologous genes. Results: TP53(32.32%) has the highest mutation rate in lung cancer, followed by EGFR(29.12%). The copy number variability(CNV) of genes:KRAS, LRP1 B, CDKN2 A, KMT2 C, FAT1, PIK3 CA, RB1, ERBB4, GRIN2 A and KDR between each pathological type is statically significant(P<0.05). The gene differential expression rate between adenocarcinoma and squamous carcinoma of gene TP53, KRAS, LRP1 B, CDKN2 A, STK11, FAT4, KMT2 D, NFE2 L2, KEAP1,PIK3 CA, RB1, ERBB4, SMARCA4 and KDR are statistically significant(P<0.05).The similarity of the protein sequence of EGFR and ERBB4 can reach 93%, and FAT4 and FAT1 are 81%. For small cell carcinoma, there's no difference in CNV between the two groups of homologous genes, and no difference between FAT4 and FAT1 in adenocarcinoma. Conclusion: The CNV and gene expression of lung cancer-associated genes are relevant to pathologic types. GFR and ERBB4 are homologous, FAT4 and FAT1 are also among the top 20 mutation genes.Additionally, there's no difference in CNV between the two groups of small cell carcinoma, which is the same between FAT4 and FAT1 in adenocarcinoma.展开更多
文摘目的系统评估孕前体质量指数与后代先天性心脏病的关联。方法系统检索PubMed、EMbase、Web of Scicence、万方、维普和中国知网,检索时间为建库至2017年12月,按纳入和排除标准筛选文献。采用NOS量表评估文献质量。结果共16篇研究纳入本Meta分析,其中病例对照研究13篇,队列研究3篇。低体质量(OR:1.03,95%CI:0.97~1.10)和超重(OR:1.05,95%CI:0.98~1.14)与后代先天性心脏病的发生风险无关,肥胖(OR:1.37,95%CI:1.17~1.61)可增加后代先天性心脏病的发生风险。剂量反应的Meta分析提示两者间存在非线性剂量反应关系,即随着孕前体质量指数的增加,后代发生先天性心脏病的风险也随之增加。结论肥胖影响后代先天性心脏病的发生风险,孕前体质量指数越大,后代发生先天性心脏病的风险越高。
基金the Construct Program of the Key Discipline in Hunan ProvinceScience and Technology Plan Project of Changsha,grant number:kq2001025,2020-2021+2 种基金the National Natural Science Foundation of China,grant number:811773530the National Natural Science Foundation of Hunan Province,grant number:2020JJ405Educational Department Project of Hunan Province,grant number:JG2018B041
文摘In December 2019, Wuhan, the capital of Hubei Province in China,reported a series of unexplained cases of pneumonia, which has been confirmed to be an acute infectious respiratory disease caused by Novel Corona Virus 2019(2019-nCoV)infection. The World Health Organization(WHO) named this pneumonia Corona Virus Disease 2019(COVID-19). The 21 st century had witnessed the spread of two previously unrecognized coronaviruses around the world with high pathogenicity before, namely severe acute respiratory syndrome coronavirus(SARS-CoV) and Middle East respiratory syndrome coronavirus(MERS-CoV) respectively. At present, 2019-nCoV ’s genome has been sequenced by researchers all over the world represented by China. Based on the systematic review and analysis of the genomic sequence characteristics of three highly pathogenic coronaviruses, this paper expounds the basic characteristics of the genomes of SARS-CoV, MERS-CoV and 2019-nCoV,which all belong to β-coronaviruses, the functionally important ORFs, ORF1 a, ORF1 b and major structural proteins including the spike(S), membrane(M) and envelop(E) and nucleiccapsid(N) proteins have no significant difference with each other. However, as the majority of genetic variations being seen at the N-terminal part of S protein, 2019-nCoV aligned best with the bat SARS-like virus bat-SL-CoVZX45. And Structure analysis of the spike(S) protein of2019-nCoV showed that it’s S protein only binds weakly to the Angiotensin Converting Enzyme2(ACE2) receptor on human cells whereas the human SARS-Cov exhibits high affinity to the ACE receptor and MERS-Cov does not need ACE2 to infect human cells,its receptor on human cells is DPP4. In the future, we hope to provide new ideas for the prevention and treatment of coronavirus by strengthening the research of coronavirus S protein and ACE2 receptor, and continuous surveillance and genomic characterization of coronaviruses from bats are necessary due to potential risks of human infection induced by a genetic mutation.
基金Graduate Student Research and Innovation Project of Hunan Provincegrant number:CX2015B182Educational Reform Project of Hunan Provincial Department of Education
文摘This study aims to investigate the association of SNPs with tumor-associated latent loci. By analyzing the effect of gene mutations on the structure and function of proteins with the database, to speculate the destructive mutants. It was found that individuals with the genotype of the five key SNP loci were more susceptible to tumors; these specific markers can be used as a molecular marker to determine the susceptibility of a tumor to the individual, and to diagnose the population with high risk of tumor; and rs201154887 can be used as a new disease molecular marker.
基金the Funding of Hunan Provincial Department of Educationgrant number:[2014]No.247[2016]No.400
文摘Objective: To investigate the relationship between gene mutation and pathological type of lung cancer, inspect and verify the consistency between homologous genes mutation in various pathologic type. Methods: Combined with the COSMIC and Uni Prot database, we obtained the reported overall big-sample mutation data of lung cancer and the protein sequences of the top 20 mutated genes,respectively. Analyze the data and cluster the protein sequences and then deduce the homologous gene. Ultimately, analyze the mutations of different pathological types of homologous genes. Results: TP53(32.32%) has the highest mutation rate in lung cancer, followed by EGFR(29.12%). The copy number variability(CNV) of genes:KRAS, LRP1 B, CDKN2 A, KMT2 C, FAT1, PIK3 CA, RB1, ERBB4, GRIN2 A and KDR between each pathological type is statically significant(P<0.05). The gene differential expression rate between adenocarcinoma and squamous carcinoma of gene TP53, KRAS, LRP1 B, CDKN2 A, STK11, FAT4, KMT2 D, NFE2 L2, KEAP1,PIK3 CA, RB1, ERBB4, SMARCA4 and KDR are statistically significant(P<0.05).The similarity of the protein sequence of EGFR and ERBB4 can reach 93%, and FAT4 and FAT1 are 81%. For small cell carcinoma, there's no difference in CNV between the two groups of homologous genes, and no difference between FAT4 and FAT1 in adenocarcinoma. Conclusion: The CNV and gene expression of lung cancer-associated genes are relevant to pathologic types. GFR and ERBB4 are homologous, FAT4 and FAT1 are also among the top 20 mutation genes.Additionally, there's no difference in CNV between the two groups of small cell carcinoma, which is the same between FAT4 and FAT1 in adenocarcinoma.
