目的建立一种检测登革病毒(dengue virus,DENV)特异性抗体的双抗原夹心ELISA方法。方法利用毕赤酵母系统表达4个血清型登革病毒包膜蛋白Ⅲ区(envelope protein domainⅢ,EDⅢ),并采用改良过碘酸钠法对EDⅢ蛋白标记辣根过氧化物酶(Horser...目的建立一种检测登革病毒(dengue virus,DENV)特异性抗体的双抗原夹心ELISA方法。方法利用毕赤酵母系统表达4个血清型登革病毒包膜蛋白Ⅲ区(envelope protein domainⅢ,EDⅢ),并采用改良过碘酸钠法对EDⅢ蛋白标记辣根过氧化物酶(Horseradish peroxidase,HRP),建立一种可同时检测4个血清型登革病毒EDⅢ蛋白特异性抗体的双抗原夹心ELISA法。并对2014年广州珠江医院门诊和住院确诊的登革热患者血清标本进行检测,并与澳洲Panbio MAC ELISA检测的敏感性进行比较。结果本研究成功建立了一种检测4个血清型登革病毒EDⅢ蛋白特异性抗体的双抗原夹心ELISA法,该方法能同时检测到4个血清型登革病毒EDⅢ蛋白免疫的小鼠血清和Ⅰ型登革病毒EDⅢ蛋白免疫的兔血清,而与烟曲霉AF-MP蛋白免疫的小鼠血清和兔血清均无交叉反应。对184份健康人血清标本检测全为阴性,而对168份确诊为登革病毒感染患者血清标本检测结果表明,两种诊断方法检测结果差异无统计学意义,P<0.001(57.1%vs 57.7%),联合NS1抗原检测方法,其敏感性提高到97.6%。结论双抗原夹心ELISA法检测登革病毒EDⅢ特异性抗体具有高度的特异性,但如果能同时联合抗原检测可明显提高登革热的早期诊断率。展开更多
Teachers are the top priority to develop the education.The thesis,first of all,has structured the index system of current teachers’construction situation in the new era.Then,the thesis has adopted the independent sam...Teachers are the top priority to develop the education.The thesis,first of all,has structured the index system of current teachers’construction situation in the new era.Then,the thesis has adopted the independent sample test and analysis of variance to analyze the difference of current teachers’construction situation in different dimensions on the basis of questionnaire data collected from teachers in five provinces and cities in the new era.Finally,the thesis has given some suggestions on improving teachers’situation in the new era from four aspects such as the improvement of the treatment guarantee mechanism for primary and middle school teachers,the operation of the cooperation mechanism among colleges,government and primary and middle school in cultivating students,the formation of training system on teachers’personal growth process and the establishment of the evaluation standard of teachers’titles as well as the standard evaluation procedure of professional titles.展开更多
文摘目的建立一种检测登革病毒(dengue virus,DENV)特异性抗体的双抗原夹心ELISA方法。方法利用毕赤酵母系统表达4个血清型登革病毒包膜蛋白Ⅲ区(envelope protein domainⅢ,EDⅢ),并采用改良过碘酸钠法对EDⅢ蛋白标记辣根过氧化物酶(Horseradish peroxidase,HRP),建立一种可同时检测4个血清型登革病毒EDⅢ蛋白特异性抗体的双抗原夹心ELISA法。并对2014年广州珠江医院门诊和住院确诊的登革热患者血清标本进行检测,并与澳洲Panbio MAC ELISA检测的敏感性进行比较。结果本研究成功建立了一种检测4个血清型登革病毒EDⅢ蛋白特异性抗体的双抗原夹心ELISA法,该方法能同时检测到4个血清型登革病毒EDⅢ蛋白免疫的小鼠血清和Ⅰ型登革病毒EDⅢ蛋白免疫的兔血清,而与烟曲霉AF-MP蛋白免疫的小鼠血清和兔血清均无交叉反应。对184份健康人血清标本检测全为阴性,而对168份确诊为登革病毒感染患者血清标本检测结果表明,两种诊断方法检测结果差异无统计学意义,P<0.001(57.1%vs 57.7%),联合NS1抗原检测方法,其敏感性提高到97.6%。结论双抗原夹心ELISA法检测登革病毒EDⅢ特异性抗体具有高度的特异性,但如果能同时联合抗原检测可明显提高登革热的早期诊断率。
基金Entrusted by the department of policy and regulation of Ministry of Education.Topic:Research on the Policies of the Construction of Teachers in New Era。
文摘Teachers are the top priority to develop the education.The thesis,first of all,has structured the index system of current teachers’construction situation in the new era.Then,the thesis has adopted the independent sample test and analysis of variance to analyze the difference of current teachers’construction situation in different dimensions on the basis of questionnaire data collected from teachers in five provinces and cities in the new era.Finally,the thesis has given some suggestions on improving teachers’situation in the new era from four aspects such as the improvement of the treatment guarantee mechanism for primary and middle school teachers,the operation of the cooperation mechanism among colleges,government and primary and middle school in cultivating students,the formation of training system on teachers’personal growth process and the establishment of the evaluation standard of teachers’titles as well as the standard evaluation procedure of professional titles.
