Accurate genomic information is essential for advancing genetic breeding research in specific rice varieties.This study presented a gapless genome assembly of the indica rice cultivar Zhonghui 8015(ZH8015)using Pac Bi...Accurate genomic information is essential for advancing genetic breeding research in specific rice varieties.This study presented a gapless genome assembly of the indica rice cultivar Zhonghui 8015(ZH8015)using Pac Bio HiFi,Hi-C,and ONT(Oxford Nanopore Technologies)ultra-long sequencing technologies,annotating 43037 gene structures.Subsequently,utilizing this genome along with transcriptomic and metabolomic techniques,we explored ZH8015's response to brown planthopper(BPH)infestation.Continuous transcriptomic sampling indicated significant changes in gene expression levels around 48 h after BPH feeding.Enrichment analysis revealed particularly significant alterations in genes related to reactive oxygen species scavenging and cell wall formation.Metabolomic results demonstrated marked increases in levels of several monosaccharides,which are components of the cell wall and dramatic changes in flavonoid contents.Omics association analysis identified differentially expressed genes associated with key metabolites,shedding light on ZH8015's response to BPH infestation.In summary,this study constructed a reliable genome sequence resource for ZH8015,and the preliminary multi-omics results will guide future insect-resistant breeding research.展开更多
Many excellent genes in wild rice have been lost during the domestication of wild rice to cultivated rice.In this study,introgression lines(ILs)were produced with a wild rice(Oryza rufipogon)accession,BJ194,as a donor...Many excellent genes in wild rice have been lost during the domestication of wild rice to cultivated rice.In this study,introgression lines(ILs)were produced with a wild rice(Oryza rufipogon)accession,BJ194,as a donor parent and an indica restorer line,Zhonghui 8015(ZH8015),as a recipient parent to map QTLs for plant height.We identified four QTLs(qPH3.1,qPH3.2,qPH2,and qPH8)related to plant height distributed on chromosomes 2,3 and 8.Furthermore,we sequenced and analyzed qPH3.2 located in the interval of RM15753-RM3525,and found this QTL may be a new locus regulating rice plant height.展开更多
基金supported by the Chinese Academy of Agricultural Sciences Innovation Project(Grant No.CAASASTIP-2013CNRRI)Fundamental Research Funds for Central Public Welfare Research Institutes of Chinese Rice Research Institute(Grant No.CPSIBRF-CNRRI-202102)。
文摘Accurate genomic information is essential for advancing genetic breeding research in specific rice varieties.This study presented a gapless genome assembly of the indica rice cultivar Zhonghui 8015(ZH8015)using Pac Bio HiFi,Hi-C,and ONT(Oxford Nanopore Technologies)ultra-long sequencing technologies,annotating 43037 gene structures.Subsequently,utilizing this genome along with transcriptomic and metabolomic techniques,we explored ZH8015's response to brown planthopper(BPH)infestation.Continuous transcriptomic sampling indicated significant changes in gene expression levels around 48 h after BPH feeding.Enrichment analysis revealed particularly significant alterations in genes related to reactive oxygen species scavenging and cell wall formation.Metabolomic results demonstrated marked increases in levels of several monosaccharides,which are components of the cell wall and dramatic changes in flavonoid contents.Omics association analysis identified differentially expressed genes associated with key metabolites,shedding light on ZH8015's response to BPH infestation.In summary,this study constructed a reliable genome sequence resource for ZH8015,and the preliminary multi-omics results will guide future insect-resistant breeding research.
基金supported by the National Natural Science Foundation of China (Grant No. 31961143016)the National Rice Industry Technology System of China (Grant No.CARS-01-03)+2 种基金the ‘14th Five-Year Plan’ Major Special Projects for Breeding New Rice Varieties of Zhejiang Province, China (Grant No. 2021C02063-1)the Agricultural Science and Technology Innovation Program of the Chinese Academy of Agricultural Sciences (Grant No. CAAS-ASTIP2013-CNRRI)Hainan Yazhou Bay Seed Laboratory Oratory, China (Grant No. B21HJ0219)
文摘Many excellent genes in wild rice have been lost during the domestication of wild rice to cultivated rice.In this study,introgression lines(ILs)were produced with a wild rice(Oryza rufipogon)accession,BJ194,as a donor parent and an indica restorer line,Zhonghui 8015(ZH8015),as a recipient parent to map QTLs for plant height.We identified four QTLs(qPH3.1,qPH3.2,qPH2,and qPH8)related to plant height distributed on chromosomes 2,3 and 8.Furthermore,we sequenced and analyzed qPH3.2 located in the interval of RM15753-RM3525,and found this QTL may be a new locus regulating rice plant height.
