目的通过Meta分析尝试阐明白介素4(IL-4)rs2243250位点基因多态性与胃癌发病风险是否存在关联。方法计算机检索Pubmed、Embase、Web of Science、Cochrane Library database、中国知网、万方等数据库,严格按照文献纳入和排除标准,搜集...目的通过Meta分析尝试阐明白介素4(IL-4)rs2243250位点基因多态性与胃癌发病风险是否存在关联。方法计算机检索Pubmed、Embase、Web of Science、Cochrane Library database、中国知网、万方等数据库,严格按照文献纳入和排除标准,搜集符合要求的病例对照研究,在进行文献质量评价后,采用比值比(OR)及其95%可信区间(95%CI)评价IL-4 rs2243250基因多态性与胃癌的关联及其程度,并进行敏感性分析和发表偏倚评估。IL-4 rs2243250基因对比模型包括等位基因模型(T vs C)、纯合子基因模型(TT vs CC)、共显性基因模型(CT vs CC)、显性基因模型(TT+CT vs CC)、隐性基因模型(TT vs CT+CC)。结果共纳入13篇文章,病例组2407例、对照组3523例。Meta分析显示,在全人群中,IL-4 rs2243250基因多态性与胃癌发病关联的OR值差异均无统计学意义(P>0.05),但在白种人中,IL-4 rs2243250基因多态性可能增加胃癌发病的风险(TT vs CC:P=0.04,OR=1.566,95%CI为1.021~2.403;TT vs CC+CT:P=0.04,OR=1.554,95%CI为1.019~2.369)。另外,检测方法PCR-ARMS可能是潜在的异质性来源。结论在全人群中,本研究结果不支持“IL-4 rs2243250基因多态性与胃癌风险相关”,但在亚组分析中,IL-4 rs2243250基因多态性可能增加白种人群胃癌的发病风险。针对IL-4 rs2243250基因多态性与胃癌风险的关联,当前仍需持谨慎态度。展开更多
Objective:To investigate the protective effects and its possible mechanism of Wuzi Yanzong Pill(WYP) on Parkinson’s disease(PD) model mice.Methods:Thirty-six C57BL/6 male mice were randomly assigned to 3 groups inclu...Objective:To investigate the protective effects and its possible mechanism of Wuzi Yanzong Pill(WYP) on Parkinson’s disease(PD) model mice.Methods:Thirty-six C57BL/6 male mice were randomly assigned to 3 groups including normal,PD,and PD+WYP groups,12 mice in each group.One week of intraperitoneal injection of 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine(MPTP) was used to establish the classical PD model in mice.Meanwhile,mice in the PD+WYP group were administrated with 16 g/kg WYP,twice daily by gavage.After 14 days of administration,gait test,open field test and pole test were measured to evaluate the movement function.Tyrosine hydroxylase(TH) neurons in substantia nigra of midbrain and binding immunoglobulin heavy chain protein(GRP78) in striatum and cortex were observed by immunohistochemistry.The levels of TH,GRP78,p-PERK,p-elF2α,ATF4,p-IRE1α,XBP1,ATF6,CHOP,ASK1,p-JNK,Caspase-12,-9 and-3 in brain were detected by Western blot.Results:Compared with the PD group,WYP treatment ameliorated gait balance ability in PD mice(P<0.05).Similarly,WYP increased the total distance and average speed(P<0.05or P<0.01),reduced rest time and pole time(P<0.05).Moreover,WYP significantly increased TH positive cells(P<0.01).Immunofluorescence showed WYP attenuated the levels of GRP78 in striatum and cortex.Meanwhile,WYP treatment significantly decreased the protein expressions of GRP78,p-PERK,p-elF2α,ATF4,p-IRE1α,XBP1,CHOP,Caspase-12 and Caspase-9(P<0.05or P<0.01).Conclusions:WYP ameliorated motor symptoms and pathological lesion of PD mice,which may be related to the regulation of unfolded protein response-mediated signaling pathway and inhibiting the endoplasmic reticulum stress-mediated neuronal apoptosis pathway.展开更多
文摘目的通过Meta分析尝试阐明白介素4(IL-4)rs2243250位点基因多态性与胃癌发病风险是否存在关联。方法计算机检索Pubmed、Embase、Web of Science、Cochrane Library database、中国知网、万方等数据库,严格按照文献纳入和排除标准,搜集符合要求的病例对照研究,在进行文献质量评价后,采用比值比(OR)及其95%可信区间(95%CI)评价IL-4 rs2243250基因多态性与胃癌的关联及其程度,并进行敏感性分析和发表偏倚评估。IL-4 rs2243250基因对比模型包括等位基因模型(T vs C)、纯合子基因模型(TT vs CC)、共显性基因模型(CT vs CC)、显性基因模型(TT+CT vs CC)、隐性基因模型(TT vs CT+CC)。结果共纳入13篇文章,病例组2407例、对照组3523例。Meta分析显示,在全人群中,IL-4 rs2243250基因多态性与胃癌发病关联的OR值差异均无统计学意义(P>0.05),但在白种人中,IL-4 rs2243250基因多态性可能增加胃癌发病的风险(TT vs CC:P=0.04,OR=1.566,95%CI为1.021~2.403;TT vs CC+CT:P=0.04,OR=1.554,95%CI为1.019~2.369)。另外,检测方法PCR-ARMS可能是潜在的异质性来源。结论在全人群中,本研究结果不支持“IL-4 rs2243250基因多态性与胃癌风险相关”,但在亚组分析中,IL-4 rs2243250基因多态性可能增加白种人群胃癌的发病风险。针对IL-4 rs2243250基因多态性与胃癌风险的关联,当前仍需持谨慎态度。
基金Supported by the National Natural Science Foundation of China(Nos.81703978 and 81102552)the Special Fund for Science and Technology Innovation Team of Shanxi University of Chinese Medicine(No.2022TD1013)+5 种基金the Natural Science Foundation of Shanxi Province(No.201901D111334)the Returned Chinese Scholars Technology Activities Preferred Project,Shanxi Province of China(No.20200026)the Research Project supported by Shanxi Scholarship Council of China(No.2021-142)Shanxi University Science and Technology Innovation Project(No.2019L0724)the Key Science and technology R&D project of Jinzhong(No.Y213004)the Young Scientist Cultivation Program Project,Shanxi University of Chinese Medicine(No.2021PY-QN-03)。
文摘Objective:To investigate the protective effects and its possible mechanism of Wuzi Yanzong Pill(WYP) on Parkinson’s disease(PD) model mice.Methods:Thirty-six C57BL/6 male mice were randomly assigned to 3 groups including normal,PD,and PD+WYP groups,12 mice in each group.One week of intraperitoneal injection of 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine(MPTP) was used to establish the classical PD model in mice.Meanwhile,mice in the PD+WYP group were administrated with 16 g/kg WYP,twice daily by gavage.After 14 days of administration,gait test,open field test and pole test were measured to evaluate the movement function.Tyrosine hydroxylase(TH) neurons in substantia nigra of midbrain and binding immunoglobulin heavy chain protein(GRP78) in striatum and cortex were observed by immunohistochemistry.The levels of TH,GRP78,p-PERK,p-elF2α,ATF4,p-IRE1α,XBP1,ATF6,CHOP,ASK1,p-JNK,Caspase-12,-9 and-3 in brain were detected by Western blot.Results:Compared with the PD group,WYP treatment ameliorated gait balance ability in PD mice(P<0.05).Similarly,WYP increased the total distance and average speed(P<0.05or P<0.01),reduced rest time and pole time(P<0.05).Moreover,WYP significantly increased TH positive cells(P<0.01).Immunofluorescence showed WYP attenuated the levels of GRP78 in striatum and cortex.Meanwhile,WYP treatment significantly decreased the protein expressions of GRP78,p-PERK,p-elF2α,ATF4,p-IRE1α,XBP1,CHOP,Caspase-12 and Caspase-9(P<0.05or P<0.01).Conclusions:WYP ameliorated motor symptoms and pathological lesion of PD mice,which may be related to the regulation of unfolded protein response-mediated signaling pathway and inhibiting the endoplasmic reticulum stress-mediated neuronal apoptosis pathway.
