Cu,Zn SOD is a highly conserved enzyme and the controversy about its evolutionary possibility in the near future has been lively. In order to further our understanding of the future fate of human Cu,Zn SOD, we adopt...Cu,Zn SOD is a highly conserved enzyme and the controversy about its evolutionary possibility in the near future has been lively. In order to further our understanding of the future fate of human Cu,Zn SOD, we adopted a strategy relating to the directed evolution to study how the mutants of human Cu,Zn SOD respond to different oxidative stress. After five rounds of screening, we found a mutant that can survive under harsh pressures and DNA sequencing proves that it shows a mutation responsible for the phenomenon. However, under natural pressure, our screening comes to nothing. Then we may draw the following conclusions: the evolution of biological macromolecules in some respect depends on their surroundings and if they are too familiar with a certain environment, they may embody evolutionary inertia.展开更多
Liver transplantation(LT)is the only curative option for simultaneous hepatocellular carcinoma(HCC)and cirrhosis induced by hepatitis B virus(HBV)infection.However,the recurrence rate of HCC after LT is high,and intra...Liver transplantation(LT)is the only curative option for simultaneous hepatocellular carcinoma(HCC)and cirrhosis induced by hepatitis B virus(HBV)infection.However,the recurrence rate of HCC after LT is high,and intrahepatic recurrence of the primary tumor and de novo HCC occur freque ntly in the tra nspla nted liver[1].展开更多
文摘Cu,Zn SOD is a highly conserved enzyme and the controversy about its evolutionary possibility in the near future has been lively. In order to further our understanding of the future fate of human Cu,Zn SOD, we adopted a strategy relating to the directed evolution to study how the mutants of human Cu,Zn SOD respond to different oxidative stress. After five rounds of screening, we found a mutant that can survive under harsh pressures and DNA sequencing proves that it shows a mutation responsible for the phenomenon. However, under natural pressure, our screening comes to nothing. Then we may draw the following conclusions: the evolution of biological macromolecules in some respect depends on their surroundings and if they are too familiar with a certain environment, they may embody evolutionary inertia.
基金the National Natural Science Foundation of China [81372595]the National Key Basic Research Development Program [2014-CBA02001]。
文摘Liver transplantation(LT)is the only curative option for simultaneous hepatocellular carcinoma(HCC)and cirrhosis induced by hepatitis B virus(HBV)infection.However,the recurrence rate of HCC after LT is high,and intrahepatic recurrence of the primary tumor and de novo HCC occur freque ntly in the tra nspla nted liver[1].
