We have produced ultracold polar RbCs molecules via photoassociation starting from laser-cooled 85Rb and 133Cs atoms in a dual−species,forced dark magneto-optical trap.The formed electronically excited RbCs∗molecules ...We have produced ultracold polar RbCs molecules via photoassociation starting from laser-cooled 85Rb and 133Cs atoms in a dual−species,forced dark magneto-optical trap.The formed electronically excited RbCs∗molecules correlated to the Rb(5S_(1/2))+Cs(6P_(1/2))dissociation limit are observed by trap loss spectroscopy.Following the decay of these excited RbCs*molecules,the formed ground state molecules are directly ionized by a two-photon single-color pulse dye laser,which is a new ionization mechanism for ground state RbCs molecules and thence detected by time-of-flight mass spectroscopy.展开更多
Background Tetralogy of Fallot （TOF） is the most common malformation of children with an incidence of approximately 10% of congenital heart disease patients. There can be a wide spectrum to the severity of the anato...Background Tetralogy of Fallot （TOF） is the most common malformation of children with an incidence of approximately 10% of congenital heart disease patients. There can be a wide spectrum to the severity of the anatomic defects, which include ventricular septal defect, aortic override, right ventricular outflow tract obstruction, and right ventricular hypertrophy. We examined the relationship between right ventricular hypertrophy in patients with TOF and the gene expression of factors in the mitogen-activated protein kinase （MAPK） signal pathway. Methods To gain insight into the characteristic gene（s） involved in molecular mechanisms of right ventricular hypertrophy in TOF, differential mRNA and micro RNA expression profiles were assessed using expression-based micro array technology on right ventricular biopsies from young TOF patients who underwent primary correction and on normal heart tissue. We then analyzed the gene expression of the MAPK signal pathway using reverse transcription-polymerase chain reaction （RT-PCR） in normals and TOF patients. Results Using the micro RNA chip V3.0 and human whole genome oligonucleotide microarray VI.0 to detect the gene expression, we found 1068 genes showing altered expression of at least two-fold in TOF patients compared to the normal hearts, and 47 micro RNAs that showed a significant difference of at least two-fold in TOF patients. We then analyzed these mRNAs and micro RNAs by target gene predicting software Microcosm Targets version 5.0, and determined those mRNA highly relevant to the right ventricular hypertrophy by RT-PCR method. There were obvious differences in the gene expression of factors in the MAPK signal pathway when using RT-PCR, which was consistent to the results of the cDNA microarray.Conclusion The upregulation of genes in the MAPK signal pathway may be the key events that contribute to right ventricular hypertrophy and stunted angiogenesis in patients with TOF.展开更多
基金by the National Key Fundamental Basic Research Program of China(2006CB921603)Major Program of the National Natural Science Foundation of China(10934004)+2 种基金the National Natural Science Foundation of China(60978018,60808009,61008012,and 60978001)the New Teacher Fund of Ministry of Education of China(200801081021)NSFC Project for Excellent Research Team(Grant No 60821004).
文摘We have produced ultracold polar RbCs molecules via photoassociation starting from laser-cooled 85Rb and 133Cs atoms in a dual−species,forced dark magneto-optical trap.The formed electronically excited RbCs∗molecules correlated to the Rb(5S_(1/2))+Cs(6P_(1/2))dissociation limit are observed by trap loss spectroscopy.Following the decay of these excited RbCs*molecules,the formed ground state molecules are directly ionized by a two-photon single-color pulse dye laser,which is a new ionization mechanism for ground state RbCs molecules and thence detected by time-of-flight mass spectroscopy.
文摘Background Tetralogy of Fallot （TOF） is the most common malformation of children with an incidence of approximately 10% of congenital heart disease patients. There can be a wide spectrum to the severity of the anatomic defects, which include ventricular septal defect, aortic override, right ventricular outflow tract obstruction, and right ventricular hypertrophy. We examined the relationship between right ventricular hypertrophy in patients with TOF and the gene expression of factors in the mitogen-activated protein kinase （MAPK） signal pathway. Methods To gain insight into the characteristic gene（s） involved in molecular mechanisms of right ventricular hypertrophy in TOF, differential mRNA and micro RNA expression profiles were assessed using expression-based micro array technology on right ventricular biopsies from young TOF patients who underwent primary correction and on normal heart tissue. We then analyzed the gene expression of the MAPK signal pathway using reverse transcription-polymerase chain reaction （RT-PCR） in normals and TOF patients. Results Using the micro RNA chip V3.0 and human whole genome oligonucleotide microarray VI.0 to detect the gene expression, we found 1068 genes showing altered expression of at least two-fold in TOF patients compared to the normal hearts, and 47 micro RNAs that showed a significant difference of at least two-fold in TOF patients. We then analyzed these mRNAs and micro RNAs by target gene predicting software Microcosm Targets version 5.0, and determined those mRNA highly relevant to the right ventricular hypertrophy by RT-PCR method. There were obvious differences in the gene expression of factors in the MAPK signal pathway when using RT-PCR, which was consistent to the results of the cDNA microarray.Conclusion The upregulation of genes in the MAPK signal pathway may be the key events that contribute to right ventricular hypertrophy and stunted angiogenesis in patients with TOF.
