为提高黄河三角洲滨海低产田耕层有机质含量,探究施用不同材料对研究区域土壤改良效果,设置WM(木本泥炭+腐熟秸秆)、BS(生物炭+腐熟秸秆)、OS(有机肥+腐熟秸秆)、S(腐熟秸秆)、CK(常规施肥)5种不同类型改良材料处理进行田间试验,改良材...为提高黄河三角洲滨海低产田耕层有机质含量,探究施用不同材料对研究区域土壤改良效果,设置WM(木本泥炭+腐熟秸秆)、BS(生物炭+腐熟秸秆)、OS(有机肥+腐熟秸秆)、S(腐熟秸秆)、CK(常规施肥)5种不同类型改良材料处理进行田间试验,改良材料用量均为30 t hm^-2,腐熟秸秆用量为14 t hm^-2。经过两茬玉米种植后,采集土壤样品,分析了土壤微生物及养分变化。研究结果表明,木本泥炭材料+腐熟秸秆处理的Chao1、Simpson指数、Shannon指数提高,土壤微生物多样性增加。各处理中土壤细菌类群主要有Proteobacteria(变形菌门)、Acidobacteria(酸杆菌门)、Actinobacteria(放线菌门)、Chloroflexi(绿弯菌门)、Bacteroidetes(拟杆菌门),其合计量的相对丰度在5种处理WM、BS、OS、S、CK中分别为26%、16%、15%、15%、9%。在5种处理中相对占比较高的六种优势菌属为Exiguobacterium(微小杆菌属)、Citrobacter(柠檬酸菌属)、Pseudomonas(假单胞杆菌属)、Acinetobacter(不动杆菌属)、Kaistobacter、Pontibacter。冗余分析发现与有机质、可溶性有机碳等指标相关性最好的浮霉菌门(Planctomycetes)、疣微菌门(Verrucomicrobia)、芽单胞菌门(Gemmatimonadetes)微生物在腐熟秸秆处理中OTU指数最高。在施加不同改良材料处理中,木本泥炭材料+腐熟秸秆提高碱解氮、有机质含量效果显著,有机肥+腐熟秸秆处理提高土壤溶解性有机碳效果显著。展开更多
Objective: To evaluate the effect of baicalin on subarachnoid hemorrhage(SAH) in rats and explore the potential mechanisms. Methods: Sprague-Dawley rats underwent experimental SAH and received treatment with baicalin ...Objective: To evaluate the effect of baicalin on subarachnoid hemorrhage(SAH) in rats and explore the potential mechanisms. Methods: Sprague-Dawley rats underwent experimental SAH and received treatment with baicalin at 10 or 50 mg/kg after 2 and 12 h of SAH. Neurological scores, brain water content, Evans-blue extravasation, and levels of glutathione peroxidase(GSH-Px), superoxide dismutase(SOD), myeloperoxidase(MPO), and malondialdehyde(MDA) were measured 24 h after SAH. Expression of nuclear factor erythroid-related factor 2(Nrf2), NAD(P)H: quinone oxidoreductase 1(NQO1), matrix metalloproteinase-9(MMP-9), aquaporin 4(AQP4), occludin, and zonulaoccludens-1(ZO-1) were detected in the brain by Western blot. Heme oxygenase-1(HO-1) was detected by quantitative polymerase chain reaction, and tumor necrosis factor-α(TNF-α) and interleukin-1β(IL-1β) were assessed by enzyme-linked immunosorbent assay. Results: Baicalin attenuated EBI 24 h after SAH in rats(P<0.05). Baicalin elevated neurological scores, GSH-Px, SOD, and increased the expression of Nrf2, NQO1, HO-1, occludin, and ZO-1 in SAH rats(P<0.05 or P<0.01). Baicalin reduced MPO, MDA, and the expression of MMP-9, AQP4, TNF-α, and IL-1β(P<0.05 or P<0.01). Conclusion: Baicalin reduced SAH-induced EBI, partially via activation of the Nrf2/HO-1 pathway and inhibition of MMP-9 and AQP4.展开更多
文摘为提高黄河三角洲滨海低产田耕层有机质含量,探究施用不同材料对研究区域土壤改良效果,设置WM(木本泥炭+腐熟秸秆)、BS(生物炭+腐熟秸秆)、OS(有机肥+腐熟秸秆)、S(腐熟秸秆)、CK(常规施肥)5种不同类型改良材料处理进行田间试验,改良材料用量均为30 t hm^-2,腐熟秸秆用量为14 t hm^-2。经过两茬玉米种植后,采集土壤样品,分析了土壤微生物及养分变化。研究结果表明,木本泥炭材料+腐熟秸秆处理的Chao1、Simpson指数、Shannon指数提高,土壤微生物多样性增加。各处理中土壤细菌类群主要有Proteobacteria(变形菌门)、Acidobacteria(酸杆菌门)、Actinobacteria(放线菌门)、Chloroflexi(绿弯菌门)、Bacteroidetes(拟杆菌门),其合计量的相对丰度在5种处理WM、BS、OS、S、CK中分别为26%、16%、15%、15%、9%。在5种处理中相对占比较高的六种优势菌属为Exiguobacterium(微小杆菌属)、Citrobacter(柠檬酸菌属)、Pseudomonas(假单胞杆菌属)、Acinetobacter(不动杆菌属)、Kaistobacter、Pontibacter。冗余分析发现与有机质、可溶性有机碳等指标相关性最好的浮霉菌门(Planctomycetes)、疣微菌门(Verrucomicrobia)、芽单胞菌门(Gemmatimonadetes)微生物在腐熟秸秆处理中OTU指数最高。在施加不同改良材料处理中,木本泥炭材料+腐熟秸秆提高碱解氮、有机质含量效果显著,有机肥+腐熟秸秆处理提高土壤溶解性有机碳效果显著。
基金Supported by the Project of Medical Innovation of Health and Family Planning Commision of Fujian Province of China (No. 2016-CX-18)the Natural Science Foundation of Fujian Province of China (No. 2017J01204)+3 种基金the Key Young Talents Cultivation Project of Health and Family Planning Commision of Fujian Province of China (No. 2016-ZQN-28)the Excellent Youth Scientific Research Personnel Cultivation Program of Fujian Province’s High School (No. 2016B026)the Clinical Key Subject (Neurosurgery) Foundation of Fujian Medical Universitythe Key Department (Neurosurgery) Foundation from Fujian Medical University Union Hospital,China。
文摘Objective: To evaluate the effect of baicalin on subarachnoid hemorrhage(SAH) in rats and explore the potential mechanisms. Methods: Sprague-Dawley rats underwent experimental SAH and received treatment with baicalin at 10 or 50 mg/kg after 2 and 12 h of SAH. Neurological scores, brain water content, Evans-blue extravasation, and levels of glutathione peroxidase(GSH-Px), superoxide dismutase(SOD), myeloperoxidase(MPO), and malondialdehyde(MDA) were measured 24 h after SAH. Expression of nuclear factor erythroid-related factor 2(Nrf2), NAD(P)H: quinone oxidoreductase 1(NQO1), matrix metalloproteinase-9(MMP-9), aquaporin 4(AQP4), occludin, and zonulaoccludens-1(ZO-1) were detected in the brain by Western blot. Heme oxygenase-1(HO-1) was detected by quantitative polymerase chain reaction, and tumor necrosis factor-α(TNF-α) and interleukin-1β(IL-1β) were assessed by enzyme-linked immunosorbent assay. Results: Baicalin attenuated EBI 24 h after SAH in rats(P<0.05). Baicalin elevated neurological scores, GSH-Px, SOD, and increased the expression of Nrf2, NQO1, HO-1, occludin, and ZO-1 in SAH rats(P<0.05 or P<0.01). Baicalin reduced MPO, MDA, and the expression of MMP-9, AQP4, TNF-α, and IL-1β(P<0.05 or P<0.01). Conclusion: Baicalin reduced SAH-induced EBI, partially via activation of the Nrf2/HO-1 pathway and inhibition of MMP-9 and AQP4.
