Newcastle disease virus-based MERS-CoV candidate vaccine elicits high-level and lasting neutralizing antibodies in Bactrian camels

Middle East respiratory syndrome coronavirus （MERS-CoV）, a member of the Coronavifidae family, is the causative pathogen for MERS that is characterized by high fever, pneumonia, acute respiratory distress syndrome （ARDS）, as well as extrapul- monary manifestations. Currently, there are no approved treatment regimens or vaccines for MERS. Here~ we generated recombinant nonvirulent Newcastle disease virus （NDV） LaSota strain expressing MERS-CoV S protein （designated as rLa- MERS-S）, and evaluated its immunogenicity in mice and Bactrian camels. The results revealed that rLa-MERS-S showed similar growth properties to those of LaSota in embryonated chicken eggs, while animal immunization studies showed that rLa-MERS-S induced MERS-CoV neutralizing antibodies in mice and camels. Our findings suggest that recombinant rLa- MERS-S may be a potential MERS-CoV veterinary vaccine candidate for camels and other animals affected by MERS.

PCB集成化RFID标签天线的优化设计

针对PCB集成化RFID标签天线设计中天线结构与标签性能之间的复杂关系,提出一种基于正交设计的标签天线优化设计。通过对PCB集成化RFID标签的回波损耗的仿真试验,经过方差分析得到标签天线各类结构变化对于试验指标影响的显著性,指导设计者找到设计指标主导因素并可获得最佳因素水平。试验结果表明该优化设计方法能够在帮助设计者提供设计方向的同时有效减小设计者的工作量,实用高效。