An exo Probe-based Reverse Transcription and Recombinase Polymerase Amplification for Rapid Detection of Feline Calicivirus in Cats

Feline calicivirus(FCV)was a highly prevalent RNA virus causing upper respiratory tract infections in cats through the mouth and nose around the world,result in the death of cats especially under 1 year old with upper respiratory tract disease(URTD)and virulent systemic disease(FCV-VSD)[1].Infected cats usually presented with mouth ulcers,salivation and gingivitis-stomatitis.Furthermore,both recovered cats and asymptomatic carriers can still carry and spread the FCV virus for extended periods of time,contributing to the potential for outbreak.This presented a significant threat to the health and lives of cats,and rare wildlife and took a heavy financial loss on the pet industry,making the prevention and control of the virus difficult.Hence,rapid identification of FCV in clinical samples was necessary to prevent the spread of FCV and avoid financial loss.

小剂量多巴酚丁胺负荷超声评价急性心肌梗死后存活心肌

目的探讨小剂量多巴酚丁胺负荷超声(LDDSE)评价急性心肌梗死(AMl)后存活心肌的价值及安全性。方法选择25例行急诊经皮冠状动脉介入治疗(PCI)AMI患者,PCI后14天进行LDDSE试验,以PCI术后3个月超声心动图检查室壁运动改善为评价存活心肌的金标准,观察LDDSE评价存活心肌的准确度,同时观察AMI早期行LDDSE的安全性。结果 LDDSE检测存活心肌的敏感度为82.5%,特异度78.5%,准确度为80.9%。在LDDSE过程中未见严重不良反应。结论 LDDSE可用于AMI患者存活心肌的评估,是一种较为安全、简便的评价存活心肌的方法。