为筛选κ-酪蛋白优势基因型,本研究设计4个SNP位点,利用基因组检测已知CSN3基因型的13头牛,采集抗凝血,提取基因组DNA,采用竞争性等位基因特异性聚合酶链式反应(Kompetitive Allele Specific PCR,KASP)对样本进行分型,CSN3基因有AA、BB...为筛选κ-酪蛋白优势基因型,本研究设计4个SNP位点,利用基因组检测已知CSN3基因型的13头牛,采集抗凝血,提取基因组DNA,采用竞争性等位基因特异性聚合酶链式反应(Kompetitive Allele Specific PCR,KASP)对样本进行分型,CSN3基因有AA、BB、EE、AB、AE、BE六种基因型。本研究实现了对北京地区213头荷斯坦种公牛及1003头母牛中CSN3三种常见变异体的全面筛选,从育种角度出发,基于κ-酪蛋白不同基因型与奶酪生产的产量和质量相关性,利用分子检测方法筛选与优良性状的优势基因型,发掘荷斯坦牛特色优质种质资源,为牧场选择合适的荷斯坦牛,提高乳蛋白率,从种源解决相关领域的技术瓶颈。展开更多
Columnar jointed rock mass with unique geometric and geological properties is one spectacular example of geometrical order in nature.Columnar joints are generally accepted to be formed by spatially uniform volume cont...Columnar jointed rock mass with unique geometric and geological properties is one spectacular example of geometrical order in nature.Columnar joints are generally accepted to be formed by spatially uniform volume contraction during cooling.In this paper,substantial field work was performed to study the geological characteristics of irregular columnar jointed basalt on the left bank dam foundation in the Baihetan Hydropower Station,where the columnar jointed rock mass is extensively exposed due to excavation.The quantitative measurements of the sizing of polygonal crack pattern of columnar joints and assessment of their degree of irregularity were summarized.Considering the irregularity of polygonal crack pattern,a modified Voronoi polygon(MVP)method was developed to model the special polygonal crack pattern of columnar joints.The new polygonal pattern obtained by the MVP method consists of a large number of irregular polygons,of which the degree of irregularity is consistent with the field measurement results.This method can reproduce the rapid evolution from an initial ideal regular hexagonal pattern to a final actual irregular polygonal pattern as the degree of irregularity increases.The compression tests of columnar jointed rock mass with different irregularity show that the geometric irregularity has a great influence on its mechanical properties.This numerical construction method provides a reliable way to reconstruct columnar joint structure with specific polygonal crack pattern,which is consistent with onsite columnar jointed basalt.展开更多
文摘为筛选κ-酪蛋白优势基因型,本研究设计4个SNP位点,利用基因组检测已知CSN3基因型的13头牛,采集抗凝血,提取基因组DNA,采用竞争性等位基因特异性聚合酶链式反应(Kompetitive Allele Specific PCR,KASP)对样本进行分型,CSN3基因有AA、BB、EE、AB、AE、BE六种基因型。本研究实现了对北京地区213头荷斯坦种公牛及1003头母牛中CSN3三种常见变异体的全面筛选,从育种角度出发,基于κ-酪蛋白不同基因型与奶酪生产的产量和质量相关性,利用分子检测方法筛选与优良性状的优势基因型,发掘荷斯坦牛特色优质种质资源,为牧场选择合适的荷斯坦牛,提高乳蛋白率,从种源解决相关领域的技术瓶颈。
基金Projects(51621006,51779251)supported by the National Natural Science Foundation of China。
文摘Columnar jointed rock mass with unique geometric and geological properties is one spectacular example of geometrical order in nature.Columnar joints are generally accepted to be formed by spatially uniform volume contraction during cooling.In this paper,substantial field work was performed to study the geological characteristics of irregular columnar jointed basalt on the left bank dam foundation in the Baihetan Hydropower Station,where the columnar jointed rock mass is extensively exposed due to excavation.The quantitative measurements of the sizing of polygonal crack pattern of columnar joints and assessment of their degree of irregularity were summarized.Considering the irregularity of polygonal crack pattern,a modified Voronoi polygon(MVP)method was developed to model the special polygonal crack pattern of columnar joints.The new polygonal pattern obtained by the MVP method consists of a large number of irregular polygons,of which the degree of irregularity is consistent with the field measurement results.This method can reproduce the rapid evolution from an initial ideal regular hexagonal pattern to a final actual irregular polygonal pattern as the degree of irregularity increases.The compression tests of columnar jointed rock mass with different irregularity show that the geometric irregularity has a great influence on its mechanical properties.This numerical construction method provides a reliable way to reconstruct columnar joint structure with specific polygonal crack pattern,which is consistent with onsite columnar jointed basalt.
