The thermal behavior of [Tb_2( m -MBA)_6(phen)_2](H_2O)_2( m -MBA=C_8H_7O_2, methoxybenzoate; phen=C_ 12 H_8N_2, 1,10-phenanthroline) in static air atmosphere was investigated by means of TG-DTG and DTA methods. The...The thermal behavior of [Tb_2( m -MBA)_6(phen)_2](H_2O)_2( m -MBA=C_8H_7O_2, methoxybenzoate; phen=C_ 12 H_8N_2, 1,10-phenanthroline) in static air atmosphere was investigated by means of TG-DTG and DTA methods. The thermal decomposition of the title compound takes place mainly in two steps. The intermediate and the residue for each decomposition were identified by the TG curve. By the kinetic method of processing thermal analysis data put forward by Malek et al ., it is defined that the kinetics model for the first-step thermal decomposition is SB( m,n ).展开更多
目的建立超高效液相色谱-串联质谱法(ultra performance liquid chromatography-tandem mas s spectrometry,UPLC-MS/MS)同时测定鳗鱼中13种磺胺类、15种喹诺酮类和4种四环素类兽药残留的分析方法。方法鳗鱼试样采用乙二胺四乙酸二钠(et...目的建立超高效液相色谱-串联质谱法(ultra performance liquid chromatography-tandem mas s spectrometry,UPLC-MS/MS)同时测定鳗鱼中13种磺胺类、15种喹诺酮类和4种四环素类兽药残留的分析方法。方法鳗鱼试样采用乙二胺四乙酸二钠(ethylenediaminetetraacetic acid disodium salt,Na;EDTA)-Mcllvaine提取液进行提取,HLB固相萃取柱净化,Waters ACQUITY UPLC BEH C_(18)色谱柱分离,以0.1%甲酸乙腈和0.1%甲酸水溶液作为流动相进行梯度洗脱;电喷雾正离子模式下,以多反应监测(multiple reaction monitoring,MRM)方式采集数据进行定性与定量分析,外标法定量。结果磺胺类、喹诺酮类及四环素类药物分别在2~50、2~50、5~100μg/kg浓度范围内线性相关性良好,相关系数均大于0.99;不同加标水平的平均回收率为82.6%~110.2%,相对标准偏差(relative standard deviations,RSDs)为3.2%~13.9%,检出限(limits of detection,LODs)和定量限(limits of quantification,LOQs)分别为0.3~2.0μg/kg和1.0~5.0μg/kg。结论本方法快速简便、灵敏可靠,适合大批量鳗鱼中同时快速对磺胺类、喹诺酮类及四环素类兽药残留进行定性和定量检测。展开更多
Fruit yield is the most important horticultural trait of tomato.SIMBP21,a SEPALLATA subclass MADS-box gene has been reported to have functions in regulating pedicel abscission zone identity and development and control...Fruit yield is the most important horticultural trait of tomato.SIMBP21,a SEPALLATA subclass MADS-box gene has been reported to have functions in regulating pedicel abscission zone identity and development and controlling sepal size in tomato.However,we generated transgenic tomato plants which overexpress SIMBP21 and found the transformants displayed curly leaves,abnormally shaped flowers with twisted and opened stamens,reduced yield parameters,and small and light seeds.Our studies on the gain-of-function phenotype and gene expression level showed that its novel aspects played important roles in determining leaf morphology,flower and inflorescence architecture,and seed size,as well as the fruit yield.Overexpression of SIMBP21 in tomato resulted in curly leaves with fewer leaflets due to the regulation of the critical leaf polarity genes that cause an imbalance between the midvein adaxial-abaxial cell growth.Defects in the architecture of flowers and inflorescences resulted in reduced fruit set.Furthermore,we demonstrated that SIMBP21 plays its role through inhibiting the expression of the genes involved in the determination of seed development in tomato and SIMBP21 protein can interact with other MADS-box protein(SIAGL11,TAGL1 and SIMBP3)to control seed size.Thus,these results suggest that overexpression of SIMBP21 causes multiple types of damage to plant growth and development,especially fruit yield,in tomato.展开更多
基金Supported by the Natural Science Foundation of Hebei Province(No.2 0 2 140 ) and Hebei Education Departm ent(No.2 0 0 112 1)
文摘The thermal behavior of [Tb_2( m -MBA)_6(phen)_2](H_2O)_2( m -MBA=C_8H_7O_2, methoxybenzoate; phen=C_ 12 H_8N_2, 1,10-phenanthroline) in static air atmosphere was investigated by means of TG-DTG and DTA methods. The thermal decomposition of the title compound takes place mainly in two steps. The intermediate and the residue for each decomposition were identified by the TG curve. By the kinetic method of processing thermal analysis data put forward by Malek et al ., it is defined that the kinetics model for the first-step thermal decomposition is SB( m,n ).
文摘目的建立超高效液相色谱-串联质谱法(ultra performance liquid chromatography-tandem mas s spectrometry,UPLC-MS/MS)同时测定鳗鱼中13种磺胺类、15种喹诺酮类和4种四环素类兽药残留的分析方法。方法鳗鱼试样采用乙二胺四乙酸二钠(ethylenediaminetetraacetic acid disodium salt,Na;EDTA)-Mcllvaine提取液进行提取,HLB固相萃取柱净化,Waters ACQUITY UPLC BEH C_(18)色谱柱分离,以0.1%甲酸乙腈和0.1%甲酸水溶液作为流动相进行梯度洗脱;电喷雾正离子模式下,以多反应监测(multiple reaction monitoring,MRM)方式采集数据进行定性与定量分析,外标法定量。结果磺胺类、喹诺酮类及四环素类药物分别在2~50、2~50、5~100μg/kg浓度范围内线性相关性良好,相关系数均大于0.99;不同加标水平的平均回收率为82.6%~110.2%,相对标准偏差(relative standard deviations,RSDs)为3.2%~13.9%,检出限(limits of detection,LODs)和定量限(limits of quantification,LOQs)分别为0.3~2.0μg/kg和1.0~5.0μg/kg。结论本方法快速简便、灵敏可靠,适合大批量鳗鱼中同时快速对磺胺类、喹诺酮类及四环素类兽药残留进行定性和定量检测。
基金the Training Program of Chongqing University Bioengineering College,China(0221001105301)the National Natural Science Foundation of China(31872121 and 31801870).
文摘Fruit yield is the most important horticultural trait of tomato.SIMBP21,a SEPALLATA subclass MADS-box gene has been reported to have functions in regulating pedicel abscission zone identity and development and controlling sepal size in tomato.However,we generated transgenic tomato plants which overexpress SIMBP21 and found the transformants displayed curly leaves,abnormally shaped flowers with twisted and opened stamens,reduced yield parameters,and small and light seeds.Our studies on the gain-of-function phenotype and gene expression level showed that its novel aspects played important roles in determining leaf morphology,flower and inflorescence architecture,and seed size,as well as the fruit yield.Overexpression of SIMBP21 in tomato resulted in curly leaves with fewer leaflets due to the regulation of the critical leaf polarity genes that cause an imbalance between the midvein adaxial-abaxial cell growth.Defects in the architecture of flowers and inflorescences resulted in reduced fruit set.Furthermore,we demonstrated that SIMBP21 plays its role through inhibiting the expression of the genes involved in the determination of seed development in tomato and SIMBP21 protein can interact with other MADS-box protein(SIAGL11,TAGL1 and SIMBP3)to control seed size.Thus,these results suggest that overexpression of SIMBP21 causes multiple types of damage to plant growth and development,especially fruit yield,in tomato.