Background: Micronaire is a comprehensive index reflecting the fineness and maturity of cotton fiber.Micronaire is one of the important internal quality indicators of the cotton fiber and is closely related to the val...Background: Micronaire is a comprehensive index reflecting the fineness and maturity of cotton fiber.Micronaire is one of the important internal quality indicators of the cotton fiber and is closely related to the value of the cotton fiber.Understanding the genetic basis of micronaire is required for the genetic improvement of the trait.However,the genetic architecture of micronaire at the genomic level is unclear.The present genome-wide association study(GWAS)aimed to identify the genetic mechanism of the micronaire trait in 83 representa:tive upland cotton lines grown in multiple environments.Results GWAS of micronaire used 83 upland cotton accessions assayed by a Cotton 63 K Illumina Infinium single nucleotide polymorphism(SNP)array.A total of 11 quantitative trait loci(QTLs)for micronaire were detected on 10 chromosomes.These 11 QTLs included 27 identified genes with specific expression patterns.A novel QTL,qFM-A12–1,included 12 significant SNPs,and GhFLA9 was identified as a candidate gene based on haplotype block analysis and on strong and direct linkage disequilibrium between the significantly related SNPs and gene.GhFLA9 was expressed at a high level during secondary wall thickening at 20∼25 days post-anthesis.The expression level of GhFLA9 was significantly higher in the low micronaire line(Msco-12)than that in the high micronaire line(Chuangyou-9).Conclusions: This study provides a genetic reference for genetic improvement of cotton fiber micronaire and a foundation for verification of the functions of GhFLA9.展开更多
Trifloxysulfuron(Envoke)is an acetolactate synthase-inhibitor herbicide and can be used to control many broadleaf weeds and nutsedges in cotton production.However,there is a lack of information on genotypic variation ...Trifloxysulfuron(Envoke)is an acetolactate synthase-inhibitor herbicide and can be used to control many broadleaf weeds and nutsedges in cotton production.However,there is a lack of information on genotypic variation in response to the herbicide.In this field study,60 Pima(Gossypium barbadense L.)lines,122 Upland(G.hirsutum L.)lines,and 9 Upland×Pima segregating populations were divided into five tests(18A,18B,18G,18RB,and 18HQ)to evaluate tri-floxysulfuron tolerance at the 7-true leaf stage(42 days after planting)under the same field conditions in 2018.Across the five tests,Pima cotton genotypes tested in this study did not show any visual crop injury based on percentage of plants with chlorosis at 6 days after treatment(DAT),indicating consistent and high levels of trifloxysulfuron toler-ance.However,the response to trifloxysulfuron within Upland cotton is highly variable.While Upland cotton is overall more sensitive to trifloxysulfuron with crop injury up to 80%than Pima cotton,19 lines had injury below 5%including one line with no visual injury,and 19 lines had injury between 5%and 10%.In test 18HQ with 15 transgenic Upland cultivars and 17 non-transgenic Upland lines,the analysis of variance detected a significant genotypic difference.The broad-sense heritability estimates for trifloxysulfuron tolerance based on crop injury at 6 DAT was 0.555,suggesting that trifloxysulfuron tolerance in Upland cotton is moderately heritable.This study represents the first report that Pima cotton and many Upland cotton lines are highly tolerant to trifloxysulfuron with no or little crop injury.展开更多
基金The present study was funded by National Key Research and Development Program of China(grants nos.2018YFD0101402,2018YFD0100300 and 2016YFD0101400)the Natural Science Foundation of Xinjiang Uygur Autonomous Region of China(grant no.2020D01A135)Agricultural Science and Technology Innovation Program of Chinese Academy of Agricultural Sciences.
文摘Background: Micronaire is a comprehensive index reflecting the fineness and maturity of cotton fiber.Micronaire is one of the important internal quality indicators of the cotton fiber and is closely related to the value of the cotton fiber.Understanding the genetic basis of micronaire is required for the genetic improvement of the trait.However,the genetic architecture of micronaire at the genomic level is unclear.The present genome-wide association study(GWAS)aimed to identify the genetic mechanism of the micronaire trait in 83 representa:tive upland cotton lines grown in multiple environments.Results GWAS of micronaire used 83 upland cotton accessions assayed by a Cotton 63 K Illumina Infinium single nucleotide polymorphism(SNP)array.A total of 11 quantitative trait loci(QTLs)for micronaire were detected on 10 chromosomes.These 11 QTLs included 27 identified genes with specific expression patterns.A novel QTL,qFM-A12–1,included 12 significant SNPs,and GhFLA9 was identified as a candidate gene based on haplotype block analysis and on strong and direct linkage disequilibrium between the significantly related SNPs and gene.GhFLA9 was expressed at a high level during secondary wall thickening at 20∼25 days post-anthesis.The expression level of GhFLA9 was significantly higher in the low micronaire line(Msco-12)than that in the high micronaire line(Chuangyou-9).Conclusions: This study provides a genetic reference for genetic improvement of cotton fiber micronaire and a foundation for verification of the functions of GhFLA9.
基金supported in part by Cotton Incorporated and New Mexico Agricultural Experiment Station.
文摘Trifloxysulfuron(Envoke)is an acetolactate synthase-inhibitor herbicide and can be used to control many broadleaf weeds and nutsedges in cotton production.However,there is a lack of information on genotypic variation in response to the herbicide.In this field study,60 Pima(Gossypium barbadense L.)lines,122 Upland(G.hirsutum L.)lines,and 9 Upland×Pima segregating populations were divided into five tests(18A,18B,18G,18RB,and 18HQ)to evaluate tri-floxysulfuron tolerance at the 7-true leaf stage(42 days after planting)under the same field conditions in 2018.Across the five tests,Pima cotton genotypes tested in this study did not show any visual crop injury based on percentage of plants with chlorosis at 6 days after treatment(DAT),indicating consistent and high levels of trifloxysulfuron toler-ance.However,the response to trifloxysulfuron within Upland cotton is highly variable.While Upland cotton is overall more sensitive to trifloxysulfuron with crop injury up to 80%than Pima cotton,19 lines had injury below 5%including one line with no visual injury,and 19 lines had injury between 5%and 10%.In test 18HQ with 15 transgenic Upland cultivars and 17 non-transgenic Upland lines,the analysis of variance detected a significant genotypic difference.The broad-sense heritability estimates for trifloxysulfuron tolerance based on crop injury at 6 DAT was 0.555,suggesting that trifloxysulfuron tolerance in Upland cotton is moderately heritable.This study represents the first report that Pima cotton and many Upland cotton lines are highly tolerant to trifloxysulfuron with no or little crop injury.
