目的检测小鼠脑挫伤后不同时间点大麻素2型受体(cannabinoid type 2 receptor,CB2R)的表达情况,并探索其与损伤时间的相关性。方法通过PCI3000精密颅脑损伤撞击器建立小鼠脑挫伤模型,利用免疫组织化学染色、免疫荧光染色和Western印迹...目的检测小鼠脑挫伤后不同时间点大麻素2型受体(cannabinoid type 2 receptor,CB2R)的表达情况,并探索其与损伤时间的相关性。方法通过PCI3000精密颅脑损伤撞击器建立小鼠脑挫伤模型,利用免疫组织化学染色、免疫荧光染色和Western印迹法检测不同损伤时间点损伤区周围CB2R的表达变化。结果免疫组织化学染色结果显示,假手术组脑皮质中仅少量细胞呈CB2R阳性表达,脑挫伤后CB2R阳性细胞率逐渐升高,于伤后12h和伤后7d两次达到高峰,随后逐渐下降,至伤后28d基本恢复至正常水平。Western印迹法检测结果与免疫组织化学染色结果一致。免疫荧光染色结果显示,脑挫伤后CB2R阳性神经元、CB2R阳性单核细胞、CB2R阳性星形胶质细胞数与总细胞数的比值变化均呈单峰模式,分别于伤后12h、1d、7d达到高峰。结论小鼠脑挫伤后CB2R在神经元、单核细胞及星形胶质细胞中的表达提示,其可能参与调节这些细胞的生物学功能。脑挫伤后CB2R的动态表达存在时间规律性,有望成为法医学脑挫伤损伤时间推断的生物学指标。展开更多
Taking into consideration the gas compressibility and chemical reaction reversibility, a model was developed to study the interactions between gas flow and chemical reaction in porous media and resolved by the finite ...Taking into consideration the gas compressibility and chemical reaction reversibility, a model was developed to study the interactions between gas flow and chemical reaction in porous media and resolved by the finite volume method on the basis of the gas-solid reaction aA(g)+bB(s)cC(g)+dD(s).The numerical analysis shows that the equilibrium constant is an important factor influencing the process of gas-solid reaction. The stoichiometric coefficients, molar masses of reactant gas, product gas and inert gas are the main factors influencing the density of gas mixture. The equilibrium constant influences the gas flow in porous media obviously when the stoichiometric coefficients satisfy a/c≠1.展开更多
文摘目的检测小鼠脑挫伤后不同时间点大麻素2型受体(cannabinoid type 2 receptor,CB2R)的表达情况,并探索其与损伤时间的相关性。方法通过PCI3000精密颅脑损伤撞击器建立小鼠脑挫伤模型,利用免疫组织化学染色、免疫荧光染色和Western印迹法检测不同损伤时间点损伤区周围CB2R的表达变化。结果免疫组织化学染色结果显示,假手术组脑皮质中仅少量细胞呈CB2R阳性表达,脑挫伤后CB2R阳性细胞率逐渐升高,于伤后12h和伤后7d两次达到高峰,随后逐渐下降,至伤后28d基本恢复至正常水平。Western印迹法检测结果与免疫组织化学染色结果一致。免疫荧光染色结果显示,脑挫伤后CB2R阳性神经元、CB2R阳性单核细胞、CB2R阳性星形胶质细胞数与总细胞数的比值变化均呈单峰模式,分别于伤后12h、1d、7d达到高峰。结论小鼠脑挫伤后CB2R在神经元、单核细胞及星形胶质细胞中的表达提示,其可能参与调节这些细胞的生物学功能。脑挫伤后CB2R的动态表达存在时间规律性,有望成为法医学脑挫伤损伤时间推断的生物学指标。
基金Projects(51304035,50974030)supported by the National Natural Science Foundation of ChinaProject(20110491512)supported by the Postdoctoral Science Foundation of China+2 种基金Project(20130042120034)supported by the Specialized Research Fund for the Doctoral Program of Higher Education(New Teachers),ChinaProject(120401008)supported by the Fundamental Research Funds for Central Universities,ChinaProject(L20150173)supported by the Scientific Research Fund of Liaoning Provincial Education Department,China
文摘Taking into consideration the gas compressibility and chemical reaction reversibility, a model was developed to study the interactions between gas flow and chemical reaction in porous media and resolved by the finite volume method on the basis of the gas-solid reaction aA(g)+bB(s)cC(g)+dD(s).The numerical analysis shows that the equilibrium constant is an important factor influencing the process of gas-solid reaction. The stoichiometric coefficients, molar masses of reactant gas, product gas and inert gas are the main factors influencing the density of gas mixture. The equilibrium constant influences the gas flow in porous media obviously when the stoichiometric coefficients satisfy a/c≠1.
