The aim of this study is to investigate microbial structures and diversities in five active hydrothermal fields＇ sediments along the Eastern Lau Spreading Centre （ELSC） in the Lau Basin （southwest Pacific）. Micro...The aim of this study is to investigate microbial structures and diversities in five active hydrothermal fields＇ sediments along the Eastern Lau Spreading Centre （ELSC） in the Lau Basin （southwest Pacific）. Microbial communities were surveyed by denatured gradient gel electrophoresis （DGGE） and clone library analysis of 16S rRNA genes. The differences in microbial community structures among sediment samples from the five deep-sea hydrothermal sites were revealed by DGGE profiles. Cluster analysis of DGGE profiles sepa- rated the five hydrothermal samples into two groups. Four different 16S rRNA gene clone libraries, repre- senting two selected hydrothermal samples （19-4TVG8 and 19-4TVG11）, were constructed. Twenty-three and 32 phylotypes were identified from 166 and 160 bacterial clones respectively, including Proteobacteria, Bacteroidetes, Firmicutes, Nitrospirae and Planctomycetes. The phylum Proteobacteria is dominant in both bacterial libraries with a predominance of Gamma-Proteobacteria. A total of 31 and 25 phylotypes were obtained from 160 and 130 archaeal clones respectively, including Miscellaneous Crenarchaeotic Group, Marine Group Ⅰ and Ⅲ, Marine Benthic Group E, Terrestrial Hot Spring Crenarchaeota and Deep-sea Hy- drothermal Vent Euryarchaeota. These results show a variety of clones related to those involved in sulfur cycling, suggesting that the cycling and utilization of sulfur compounds may extensively occur in the Lau Basin deep-sea hydrothermal ecosystem.展开更多
As a novel three dimensional digital image correlation(3 D DIC) method, the bi-prism-based single lens(BSL) 3 D DIC method has been proposed and developed in recent years. Making use of a bi-prism, this method is able...As a novel three dimensional digital image correlation(3 D DIC) method, the bi-prism-based single lens(BSL) 3 D DIC method has been proposed and developed in recent years. Making use of a bi-prism, this method is able to perform a 3 D DIC measurement using only a single camera. Thus, the integration level of a BSL 3 D DIC system could be much higher than that of the double-camera3 D DIC system. In this paper, using a small-angle bi-prism and a camera with a longer focal length, a special BSL 3 D DIC system with a long working distance is designed for measurements in extreme environments. The principle of the system is first studied,and practical methods are then proposed for the system set-up and the determination of system parameters. Then, feasibility of the measurement system is verified by out-of-plane rigid-body translation tests. Finally, the BSL 3 D DIC system is proven to be capable of combining with a high-temperature testing instrument to perform deformation tests in a high-temperature environment of up to 1500°C.展开更多
基金Foundation item: The China Ocean Mineral Resources Research and Development Association under contract No. DYXM-115-02-2-07the State Oceanic Administration of People’s Republic of China under contract No. 200805032the National Natural Science Foundation of China under contract Nos 50621063 and 40646029
文摘The aim of this study is to investigate microbial structures and diversities in five active hydrothermal fields＇ sediments along the Eastern Lau Spreading Centre （ELSC） in the Lau Basin （southwest Pacific）. Microbial communities were surveyed by denatured gradient gel electrophoresis （DGGE） and clone library analysis of 16S rRNA genes. The differences in microbial community structures among sediment samples from the five deep-sea hydrothermal sites were revealed by DGGE profiles. Cluster analysis of DGGE profiles sepa- rated the five hydrothermal samples into two groups. Four different 16S rRNA gene clone libraries, repre- senting two selected hydrothermal samples （19-4TVG8 and 19-4TVG11）, were constructed. Twenty-three and 32 phylotypes were identified from 166 and 160 bacterial clones respectively, including Proteobacteria, Bacteroidetes, Firmicutes, Nitrospirae and Planctomycetes. The phylum Proteobacteria is dominant in both bacterial libraries with a predominance of Gamma-Proteobacteria. A total of 31 and 25 phylotypes were obtained from 160 and 130 archaeal clones respectively, including Miscellaneous Crenarchaeotic Group, Marine Group Ⅰ and Ⅲ, Marine Benthic Group E, Terrestrial Hot Spring Crenarchaeota and Deep-sea Hy- drothermal Vent Euryarchaeota. These results show a variety of clones related to those involved in sulfur cycling, suggesting that the cycling and utilization of sulfur compounds may extensively occur in the Lau Basin deep-sea hydrothermal ecosystem.
基金supported by the National Natural Science Foundation of China(Grant Nos.11672153,11232008&11227801)
文摘As a novel three dimensional digital image correlation(3 D DIC) method, the bi-prism-based single lens(BSL) 3 D DIC method has been proposed and developed in recent years. Making use of a bi-prism, this method is able to perform a 3 D DIC measurement using only a single camera. Thus, the integration level of a BSL 3 D DIC system could be much higher than that of the double-camera3 D DIC system. In this paper, using a small-angle bi-prism and a camera with a longer focal length, a special BSL 3 D DIC system with a long working distance is designed for measurements in extreme environments. The principle of the system is first studied,and practical methods are then proposed for the system set-up and the determination of system parameters. Then, feasibility of the measurement system is verified by out-of-plane rigid-body translation tests. Finally, the BSL 3 D DIC system is proven to be capable of combining with a high-temperature testing instrument to perform deformation tests in a high-temperature environment of up to 1500°C.
