In order to accelerate the product design and shorten the development cycle, the product design base on Top-Down and product level parametric design method. Based on the 2K-V type of product structure tree, the mappin...In order to accelerate the product design and shorten the development cycle, the product design base on Top-Down and product level parametric design method. Based on the 2K-V type of product structure tree, the mapping of the structure tree to the hierarchical skeleton feature tree is completed and the modeling of the~ product＇s 3D cascade skeleton is built with Pro/E skeleton Technology. The skeleton model is the basis of assembly, then to build the framework of the model＇s cascade constraints and to realize the whole model driven by the top skeleton parameters. The designers can build new models in the design of the similar structure through the rapid variant design of top skeleton parameters展开更多
文摘目的 单原子纳米酶(single-atom nanozyme,SAN)因其高原子利用率及丰富的类酶活性被广泛研究。但是目前大多数SAN活性位点负载量较低,限制了其进一步应用和发展。本研究旨在制备一种高原子负载量的SAN,并对其类酶活性进行系统研究,希望为高负载SAN的制备提供思路,并为SAN在更广泛领域的应用提供理论支持。方法 本研究通过原位锚定策略将金属盐前驱体锚定在氨基化石墨烯量子点框架中,在惰性气体保护下进行高温热解稳定Cu原子和载体之间的化学键,制备出负载量高达7.66%(质量百分比)的高负载Cu单原子纳米酶(high-loading Cu SAN)。此外,以3,3’,5,5’-四甲基联苯胺(TMB)和氮蓝四唑(NBT)为显色剂,评估了high-loading Cu SAN的类过氧化物酶(POD)、类氧化物酶(OXD)及类超氧化物歧化酶(SOD)活性,并与传统金属有机框架锚定法制备的低负载Cu单原子纳米酶(low-loading Cu SAN)作比较。以过氧化氢(H_(2)O_(2))为催化底物,对比研究了高/低负载Cu SAN的类过氧化氢酶(CAT)活性。结果 研究表明,本文制备的高负载Cu SAN的类POD和SOD活性分别是低负载Cu SAN的3.4倍和8.88倍,且表现出类酶催化选择性。结论 本研究为高负载SAN的制备和活性研究提供了思路,为SAN在检测传感、疾病治疗以及环境保护等方面的应用奠定了基础。
文摘In order to accelerate the product design and shorten the development cycle, the product design base on Top-Down and product level parametric design method. Based on the 2K-V type of product structure tree, the mapping of the structure tree to the hierarchical skeleton feature tree is completed and the modeling of the~ product＇s 3D cascade skeleton is built with Pro/E skeleton Technology. The skeleton model is the basis of assembly, then to build the framework of the model＇s cascade constraints and to realize the whole model driven by the top skeleton parameters. The designers can build new models in the design of the similar structure through the rapid variant design of top skeleton parameters
