期刊文献+
共找到4篇文章
< 1 >
每页显示 20 50 100
高效液相色谱法测定烟田土壤中二氯喹啉酸残留量 被引量:5
1
作者 石妍 肖顺 +5 位作者 周挺 梁颁捷 张珊珊 张绍升 刘国坤 顾钢 《现代农药》 CAS 2020年第5期31-35,共5页
对比了两种提取土壤中二氯喹啉酸的方法,在此基础上进行前处理方法和液相色谱条件的优化,建立了高效液相色谱法检测烟田土壤中二氯喹啉酸残留量的方法。样品用甲醇+0.05 mol/L硼砂溶液(体积比9∶1)超声振荡提取,离心后提取液用无水硫酸... 对比了两种提取土壤中二氯喹啉酸的方法,在此基础上进行前处理方法和液相色谱条件的优化,建立了高效液相色谱法检测烟田土壤中二氯喹啉酸残留量的方法。样品用甲醇+0.05 mol/L硼砂溶液(体积比9∶1)超声振荡提取,离心后提取液用无水硫酸钠去除残余水分,经浓缩、甲醇溶解、定容得待测液;用甲醇+1%乙酸水溶液(体积比65∶35)为流动相,在238 nm波长下经二极管阵列检测器检测。结果表明,在0.1、0.5、1.0 mg/kg加标水平下,二氯喹啉酸在土壤中的平均回收率为93.40%~100.94%;日内和日间精密度分别为1.38%~8.09%、5.64%~6.16%;检出限和定量限分别为0.005 mg/kg和0.025 mg/kg。 展开更多
关键词 二氯喹啉酸 高效液相色谱 烟田土壤 残留
下载PDF
烟草镰刀菌根腐病的病原鉴定 被引量:24
2
作者 吴安忠 程崖芝 +3 位作者 巫升鑫 刘国坤 张绍升 肖顺 《中国烟草学报》 EI CAS CSCD 北大核心 2018年第2期135-140,共6页
为了探明烟草镰刀菌根腐病的病原菌分类地位,2015—2017年从福建省三明市主要烟区采集的烟草根腐病病样中分离获得31个镰孢菌属菌株(F1~F31)。对所分离的菌株进行形态学甄别、系统进化分析、种特异分子鉴定及致病性测定,结果表明分离菌... 为了探明烟草镰刀菌根腐病的病原菌分类地位,2015—2017年从福建省三明市主要烟区采集的烟草根腐病病样中分离获得31个镰孢菌属菌株(F1~F31)。对所分离的菌株进行形态学甄别、系统进化分析、种特异分子鉴定及致病性测定,结果表明分离菌株显微形态特征与尖孢镰刀菌(Fusarium oxysporum)和共享镰刀菌(F.commune)相似;采用贝叶斯法(Bayesian Analysis)对4株分离菌F-04、F-18、F-19和F-28的rDNA基因内间隔区(IGS)基因作系统发育分析,结果显示这4个菌株同F.commune聚为一类并与F.oxysporum明确区分;特异性引物扩增鉴定结果表明分离菌株均为F.commune。通过室内与田间接种试验验证了分离菌株F.commune的致病性,明确其为烟草根腐病的主要病原物,这是我国首次报道共享镰刀菌F.commune引起烟草根腐病。 展开更多
关键词 烟草根腐病 病原菌 鉴定 共享镰刀菌
下载PDF
花生茎线虫对花生根的趋向、定位及侵染特性 被引量:1
3
作者 邓明雪 肖顺 +5 位作者 王文玉 程曦 程敏 侯翔宇 张绍升 刘国坤 《福建农林大学学报(自然科学版)》 CSCD 北大核心 2018年第2期148-152,共5页
采用Pluronic F-127胶体系统接种及次氯酸钠—酸性品红染色法,研究花生茎线虫对花生根部的趋向、定位及侵染特性.结果表明:花生茎线虫的幼虫及成虫均可侵入花生品种冀油7号根部,但花生根尖部对花生茎线虫无吸引作用;而对照爪哇根结线虫... 采用Pluronic F-127胶体系统接种及次氯酸钠—酸性品红染色法,研究花生茎线虫对花生根部的趋向、定位及侵染特性.结果表明:花生茎线虫的幼虫及成虫均可侵入花生品种冀油7号根部,但花生根尖部对花生茎线虫无吸引作用;而对照爪哇根结线虫2龄幼虫主要趋向、聚集于根尖伸长区并由此侵入.进一步试验表明,花生茎线虫主要聚集于花生根部的侧根基部处,并从基部侵入.花生根部表面人为切取的微小伤口和橫切根部形成的切面伤口均可吸引花生茎线虫聚集、侵入,但横切伤口处的花生茎线虫数量低于侧根基部和微小伤口处的线虫数量. 展开更多
关键词 花生茎线虫 花生 Pluronic胶系统 吸引作用 侵染部位
下载PDF
Natural Gas Types,Distribution Controlling Factors,and Future Exploration in the Western Qaidam Basin 被引量:3
4
作者 GUO Zeqing SUN Ping +5 位作者 LI Jian zhang Lin LIU Weihong TIAN Jixian zhang shaosheng ZENG Xu 《Acta Geologica Sinica(English Edition)》 SCIE CAS CSCD 2014年第4期1214-1226,共13页
The Paleogene and Neogene oil and gas in the western Qaidam basin have a regular distribution in three concentric zones from the edge to the center of the basin. Natural gas mainly occurs in the inner zone, and the ga... The Paleogene and Neogene oil and gas in the western Qaidam basin have a regular distribution in three concentric zones from the edge to the center of the basin. Natural gas mainly occurs in the inner zone, and the gas-oil ratio of the northern area of the basin is significantly higher than that of the southern area. Large amounts of carbon isotope data of natural gas, plotted in X- shaped and comprehensive identification diagrams for the southern area and northern area, respectively, were used to identify the types of natural gas. The large-scale distribution of natural gas is highly consistent with the Ro values of major source rocks, but is poorly correlated with the type of organic matter. This indicates that the main controlling factor of natural gas distribution is organic matter maturity, and the kerogen types act as the basis for the formation of different types of natural gas. Paleouplifts and squeezed anticlines near hydrocarbon generation depression centers, which are major natural gas-rich regions, control the migration directions of natural gas, while hydrocarbon migration pathways and fault systems connecting gas sources are the most important factors for natural gas reservoir formation in the inner basin. Therefore, favorable zones for natural gas distribution can be predicted on the basis of the distribution of thermal evolution and the gas generation intensity of major source rocks as well as the structural map. The Shizigou-Youshashan- Yingdong-Dawusi, Youquanzi -Kaitemilike - Youdunzi, and Xiaoliangshan - Nanyishan - Dafengshan structural belts are favorable zones for natural gas accumulation. This study has important theoretical and practical significance for future natural gas exploration. 展开更多
关键词 western Qaidam basin natural gas types distributional characteristics main controlling factors MATURITY favorable zones
下载PDF
上一页 1 下一页 到第
使用帮助 返回顶部