We study the dynamics evolution of a two-qubit Heisenberg XXX spin chain under a time-dependentrotating magnetic field.Based on the algebraic structure of the non-autonomous system,the exact solution of theSchrdinger ...We study the dynamics evolution of a two-qubit Heisenberg XXX spin chain under a time-dependentrotating magnetic field.Based on the algebraic structure of the non-autonomous system,the exact solution of theSchrdinger equation is obtained by using the method of algebraic dynamics.Based on the time-dependent analyticalsolution,we further study the entanglement evolution between the two coupled spins for different initial states,and findthat the entanglement is determined by the coefficients of the initial state and the coupling constant J of the system.展开更多
目的原核表达牛外周血淋巴细胞(peripheral blood lymphocytes,PBL)表面程序性细胞死亡蛋白-1(programmed cell death protein 1,PD-1),并制备其多抗血清。方法以PBL的cDNA为模板扩增牛PD-1胞外区片段,与载体pET-28a(+)连接,构建重组质...目的原核表达牛外周血淋巴细胞(peripheral blood lymphocytes,PBL)表面程序性细胞死亡蛋白-1(programmed cell death protein 1,PD-1),并制备其多抗血清。方法以PBL的cDNA为模板扩增牛PD-1胞外区片段,与载体pET-28a(+)连接,构建重组质粒pET-28a-PD-1,并进行生物信息学分析;重组质粒转化大肠埃希菌BL21(DE3),经不同浓度的IPTG诱导表达牛PD-1胞外区重组蛋白(简称牛PD-1蛋白),产物进行SDS-PAGE及Western-blot分析;纯化的目的蛋白免疫小鼠制备血清多抗,与CP、NCP型牛病毒性腹泻病毒(bovine viral diarrhea virus,BVDV)体外孵育牛PBL 72 h,检测BVDV病毒拷贝数。结果经双酶切及测序鉴定,重组质粒pET-28a-PD-1构建正确;牛PD-1胞外区基因读码框编码147个氨基酸,相对分子质量约为16040,其二级结构主要由α螺旋、延伸链、无规则卷曲构成,与人及鼠序列的同源性分别为79%和71%;在37℃,0.8 mmol/L IPTG诱导条件下,牛PD-1蛋白相对分子质量约19000,以包涵体的形式表达;制备的多抗血清效价最高为1∶102400,能与目的蛋白发生特异性反应,显著降低CP、NCP型BVDV病毒拷贝数(P<0.05)。结论成功构建了重组表达质粒pET-28a-PD-1,获得了牛PD-1蛋白及其多抗血清,且免疫原性较好,PD-1阻断抑制BVDV在PBL细胞中的复制,为开发具有调节及治疗慢性病毒感染的生物制品奠定基础。展开更多
基金National Natural Science Foundation of China under Grant No.10374068the Doctoral Education Fund of the Ministry of Education of China under Grant No.20050610011
文摘We study the dynamics evolution of a two-qubit Heisenberg XXX spin chain under a time-dependentrotating magnetic field.Based on the algebraic structure of the non-autonomous system,the exact solution of theSchrdinger equation is obtained by using the method of algebraic dynamics.Based on the time-dependent analyticalsolution,we further study the entanglement evolution between the two coupled spins for different initial states,and findthat the entanglement is determined by the coefficients of the initial state and the coupling constant J of the system.
