针对电网中录波型故障指示器上传的海量故障数据存在着大量的重复、干扰、错误及无效波形,提出一种基于稀疏自编码(sparse auto-encoder,SAE)的故障数据聚类清洗方法,该方法首先利用稀疏自编码对故障数据进行特征学习与降维,继而用主成...针对电网中录波型故障指示器上传的海量故障数据存在着大量的重复、干扰、错误及无效波形,提出一种基于稀疏自编码(sparse auto-encoder,SAE)的故障数据聚类清洗方法,该方法首先利用稀疏自编码对故障数据进行特征学习与降维,继而用主成分分析(principal component analysis,PCA)对降维后数据再次进行降维提取,实现对不同故障数据的特征获取;最后利用基于密度峰快速搜寻聚类算法(clustering by fast search and find of density peaks,CFSFDP)对故障特征进行聚类,实现对重复、干扰、错误等故障数据的聚类清洗和真实故障数据推送。提出的海量故障数据聚类清洗方法,达到了对不同类型故障数据进行清洗去冗的效果,为故障告警智能推送提供了技术支撑,提高了运维人员获取准确故障信息的效率。展开更多
Staphylococcus aureus is the most common etiological pathogen of bovine mastitis. The resistant strains make the disease difficult to cure. The aim of this study was to characterize the genetic nature of the antimicro...Staphylococcus aureus is the most common etiological pathogen of bovine mastitis. The resistant strains make the disease difficult to cure. The aim of this study was to characterize the genetic nature of the antimicrobial resistance in S. aureus cultured from bovine mastitis in Northwest China in 2014. A total of 44 S. aureus were isolated for antimicrobial resistance and resistance-related genes. Antimicrobial resistance was determined by disc diffusion and the corresponding resistance genes were detected by PCR. Phenotype indicated that S. aureus isolates were resistant to penicillin （84.09%）, erythromycin （20.45%）, tetracycline （15.91%）, gentamicin （9.09%）, tobramycin （6.82%）, kanamycin （6.82%） and methicillin （2.27%）. 9.09% of the S. aureus isolates were classified as multidrug resistant. In addition, genotypes showed that the isolates were resistant to rifampicin （100%, rpoB）, penicillin （95.45%, blaZ）, tetracycline （22.73%, tetK, tetM, alone or in combination）, erythromycin （22.73%, ermB or ermC）, gentamicin/tobramycin/kanamycin （2.27%, aacA-aphD）, methicillin （2.27%, mecA） and vancomycin （2.27%, vanA）. Resistance to tetracycline was attributed to the genes tetK and tetM （r=0.558, P〈0.001）. This study noted high-level geno- and phenotypic antimicrobial resistance in S. aureus isolates from bovine mastitis cases in Northwest China.展开更多
The aim of the study was to investigate the prevalence and characterization of extended-spectrum β-lactamase （ESBL）- producing Escherichia coli isolated from bovine mastitis cases in China. ChromID ESBL agar was us...The aim of the study was to investigate the prevalence and characterization of extended-spectrum β-lactamase （ESBL）- producing Escherichia coli isolated from bovine mastitis cases in China. ChromID ESBL agar was used to confirm ESBL-producing E. coli. PCR and DNA sequencing were employed to characterize the genotype of ESBL-producers. Antimicrobial susceptibility was measured by disc diffusion. Overall, 73 of 318 E. coli isolates （22.96％） were identified as ESBL-producers. Of these ESBL-producing E. coli, the prevalence of blaCTX-M and blaTEM-1 was 97.26 and 71.23％, respectively. The predominant CTX-M-type ESBL was CTX-M-15 （65.75％）, followed by CTX-M-14 （10.96％）, CTX-M-55 （9.59％）, CTX-M-64 （5.48％）, CTX-M-65 （4.11％） and CTX-M-3 （1.37％）. This study is the first report of CTX-M-64 and CTX-M-65 in E. coli isolated from bovine mastitis. Furthermore, 72 ESBL-producing E. coli isolates （98.63％） were found to be multidrug-resistance. This study noted high prevalence and rates of antimicrobial resistance of ESBL-producing E. coli isolates from bovine mastitis cases in China.展开更多
文摘针对电网中录波型故障指示器上传的海量故障数据存在着大量的重复、干扰、错误及无效波形,提出一种基于稀疏自编码(sparse auto-encoder,SAE)的故障数据聚类清洗方法,该方法首先利用稀疏自编码对故障数据进行特征学习与降维,继而用主成分分析(principal component analysis,PCA)对降维后数据再次进行降维提取,实现对不同故障数据的特征获取;最后利用基于密度峰快速搜寻聚类算法(clustering by fast search and find of density peaks,CFSFDP)对故障特征进行聚类,实现对重复、干扰、错误等故障数据的聚类清洗和真实故障数据推送。提出的海量故障数据聚类清洗方法,达到了对不同类型故障数据进行清洗去冗的效果,为故障告警智能推送提供了技术支撑,提高了运维人员获取准确故障信息的效率。
基金supported by the Central Public-Interest Scientific Institution Basal Research Fund,China (1610322015007)the Key Technology R&D Program of China during the 12th Five-Year Plan period (2012BAD12B03)the Natural Science Foundation of Gansu Province,China (145RJYA311)
文摘Staphylococcus aureus is the most common etiological pathogen of bovine mastitis. The resistant strains make the disease difficult to cure. The aim of this study was to characterize the genetic nature of the antimicrobial resistance in S. aureus cultured from bovine mastitis in Northwest China in 2014. A total of 44 S. aureus were isolated for antimicrobial resistance and resistance-related genes. Antimicrobial resistance was determined by disc diffusion and the corresponding resistance genes were detected by PCR. Phenotype indicated that S. aureus isolates were resistant to penicillin （84.09%）, erythromycin （20.45%）, tetracycline （15.91%）, gentamicin （9.09%）, tobramycin （6.82%）, kanamycin （6.82%） and methicillin （2.27%）. 9.09% of the S. aureus isolates were classified as multidrug resistant. In addition, genotypes showed that the isolates were resistant to rifampicin （100%, rpoB）, penicillin （95.45%, blaZ）, tetracycline （22.73%, tetK, tetM, alone or in combination）, erythromycin （22.73%, ermB or ermC）, gentamicin/tobramycin/kanamycin （2.27%, aacA-aphD）, methicillin （2.27%, mecA） and vancomycin （2.27%, vanA）. Resistance to tetracycline was attributed to the genes tetK and tetM （r=0.558, P〈0.001）. This study noted high-level geno- and phenotypic antimicrobial resistance in S. aureus isolates from bovine mastitis cases in Northwest China.
基金funded by the National Key R&D Program of China (2017YFD0502200)the Central PublicInterest Scientific Institution Basal Research Fund,China (1610322017013)
文摘The aim of the study was to investigate the prevalence and characterization of extended-spectrum β-lactamase （ESBL）- producing Escherichia coli isolated from bovine mastitis cases in China. ChromID ESBL agar was used to confirm ESBL-producing E. coli. PCR and DNA sequencing were employed to characterize the genotype of ESBL-producers. Antimicrobial susceptibility was measured by disc diffusion. Overall, 73 of 318 E. coli isolates （22.96％） were identified as ESBL-producers. Of these ESBL-producing E. coli, the prevalence of blaCTX-M and blaTEM-1 was 97.26 and 71.23％, respectively. The predominant CTX-M-type ESBL was CTX-M-15 （65.75％）, followed by CTX-M-14 （10.96％）, CTX-M-55 （9.59％）, CTX-M-64 （5.48％）, CTX-M-65 （4.11％） and CTX-M-3 （1.37％）. This study is the first report of CTX-M-64 and CTX-M-65 in E. coli isolated from bovine mastitis. Furthermore, 72 ESBL-producing E. coli isolates （98.63％） were found to be multidrug-resistance. This study noted high prevalence and rates of antimicrobial resistance of ESBL-producing E. coli isolates from bovine mastitis cases in China.
