为了研究煤矸石混凝土在疲劳荷载作用下的损伤特性,制作了煤矸石质量取代率分别为0、20%、40%、60%、80%、100%的混凝土试件,循环荷载取0.3 f c0和0.5 f c0,分别在循环加载2500次、5000次、7500次、10000次后测试其抗压强度和相对动弹...为了研究煤矸石混凝土在疲劳荷载作用下的损伤特性,制作了煤矸石质量取代率分别为0、20%、40%、60%、80%、100%的混凝土试件,循环荷载取0.3 f c0和0.5 f c0,分别在循环加载2500次、5000次、7500次、10000次后测试其抗压强度和相对动弹性模量。结果表明:不同质量取代率的煤矸石混凝土在循环加载次数较少时,可致密内部结构,抗压强度不但无损而且性能还有所提升,煤矸石的质量取代率越高,混凝土抗压强度越小,达到最大值所需的循环次数越少,循环10000次煤矸石质量取代率40%的混凝土抗压强度开始出现损伤,且疲劳荷载越大,损伤越大;不同质量取代率的煤矸石混凝土在循环加载初期相对动弹性模量下降较快,后期逐渐趋于稳定,疲劳荷载越大,煤矸石质量取代率越高,损伤越严重,加速了结构内部微裂隙的扩展以及新孔隙的产生。展开更多
Modified Da-chai-hu Decoction(MDD), a traditional Chinese medicinal formulation, which was empirically generated from Da-chai-hu decoction, has been utilized to treat severe acute pancreatitis(SAP) for decades. The ai...Modified Da-chai-hu Decoction(MDD), a traditional Chinese medicinal formulation, which was empirically generated from Da-chai-hu decoction, has been utilized to treat severe acute pancreatitis(SAP) for decades. The aim of the present study was to explore its potential organprotective mechanism in SAP. In the present study, rat SAP model was induced by retrograde injection of 3.5% sodium taurocholate into the biliopancreatic duct, MDD(23.35 g/kg body weight, twelve times the clinical dose) were orally given at 2 h before and 10 h after injection. At 12 h after model induction, blood was taken from vena cava for analysis of amylase, diamine oxidase(DAO), pulmonary surfactant protein-A(SP-A), and C-reactive protein(CRP). Histopathological change of pancreas, ileum and lung was assayed by H&E staining, myeloperoxidase(MPO) activity were determinated using colorimetric assay, and the expressions of occludin and nuclear factor-κB(NF-κB) were detected by real-time RT-PCR and western blot, respectively. In addition, the tissue concentrations of tumor necrosis factor-α(TNF-α), interleukin-1β(IL-1β), and monocyte chemoattractant protein-1(MCP-1) were measured by enzyme-linked immunosorbent assay(ELISA). The results showed that in SAP rats, MDD significantly alleviated histopathological damage, depressed the MPO activity and the concentrations of TNF-α, IL-1β, and MCP-1 of pancreas, ileum and lung, and reduced the serum levels of amylase [(3283.4±585.5) U·L^(-1) vs(5626.4±795.1) U·L^(-1)], DAO[(1100.1±334.3) U·L^(-1) vs(1666.4±525.3) U·L^(-1)] and CRP [(7.6±1.2) μg·mL^(-1) vs(17.8±3.8) μg·mL^(-1)]. However, the serum SP-A concentration [(106.1±16.6) pg·mL^(-1) vs(90.1±14.9) pg·mL^(-1)] was elevated when treated SAP rats with MDD. Furthermore, MDD increased the occludin expression and reduced the NF-κB expression in pancreas, ileum and lung of SAP rats. Our findings suggested that MDD administration was an effective therapeutic approach for SAP treatment. It could up-regulate occludin expression to protect intercellular tight junction and down-regulate NF-κB expression to inhibit inflammatory reaction of pancreas, ileum and lung.展开更多
Objective： To investigate the effect of combined application of Xuebijing Injection（血必净注射液, XBJ） and resolvin D1（RvD1） on survival rate and the underlying mechanisms in mice with sepsisinduced lung injury. ...Objective： To investigate the effect of combined application of Xuebijing Injection（血必净注射液, XBJ） and resolvin D1（RvD1） on survival rate and the underlying mechanisms in mice with sepsisinduced lung injury. Methods： The cecal ligation and puncture（CLP） method was used to develop a mouse sepsis model. Specific pathogen free male C57 BL/6 mice were randomly divided into 5 groups（n=20 each）： sham, CLP, CLP＋XBJ, CLP＋RvD1 and CLP＋XBJ＋RvD1. After surgery, mice in the CLP＋XBJ, CLP＋RvD1 and CLP＋XBJ＋RvD1 groups were given XBJ（25 μL/g body weight）, RvD1（10 ng/g body weight）, and their combination（the same dose of XBJ and RvD1）, respectively. In each group, 12 mice were used to observe 1-week survival rate, while the rest were executed at 12 h. Whole blood was collected for flow cytometric analysis of leukocyte adhesion molecules CD18, lung tissues were harvested for observing pathological changes, and testing the activity of myeloperoxidase（MPO） and the expression of intercellular cell adhesion molecule 1（ICAM-1）. Results： Compared with the CLP group, the histopathological damage of the lung tissues was mitigated, MPO activity was decreased in the CLP＋XBJ and CLP＋RvD1 groups（P〈0.05）. In addition, the 1-week survival rate was improved, proportion of CD18-expressing cells in whole blood and ICAM-1 protein expression in lung tissue were decreased in the CLP＋XBJ＋RvD1 group（P〈0.05 or P〈0.01）. Conclusion： XBJ together with RvD1 could effectively inhibit leukocyte adhesion, reduce lung injury, and improve the survival rate of mice with sepsis.展开更多
Objective:To investigate the effect of Modified Xiaochaihu Decoction(MXD,加味小柴胡汤)on collagen degradation in rats with chronic pancreatitis(CP).Methods:Rats were injected dibutyltin dichloride(DBTC,7 mg/kg of body...Objective:To investigate the effect of Modified Xiaochaihu Decoction(MXD,加味小柴胡汤)on collagen degradation in rats with chronic pancreatitis(CP).Methods:Rats were injected dibutyltin dichloride(DBTC,7 mg/kg of body weight)into the right caudal vein to induce CP model.Thirty heallhy male Wistar rats were randomly divided into three groups by a random number table:the control,the model and the treatment groups.Rats of treatment group were administered MXD(10 g/kg of body weight)orally once daily starting from the day post-model establishment.Pancreatic tissues were harvested after 28-day feeding and fibrosis was evaluated by picro-sirius red staining.The contents of collagen typeⅠandⅢwere detected using enzymelinked immunosorbent assay(ELISA),the expression of matrix metalloproteinase 13(MMP13)and tissue inhibitor of metalloproteinase 1(TIMP1)was analyzed by Western blot and real-time polymerase chain reaction(PCR).Results:The fibrosis scoring of pancreatic tissues,the concentrations of collagen typeⅠandⅢ,the expression levels of MMP13 and TIMP1 proteins and mRNA in the model group were all increased compared with the control group(P<0.05).After treatment with MXD,the fibrosis scoring of pancreatic tissues,the concentrations of collagen typeⅠandⅢ,the expression levels of MMP13 proteins and m RNA in the teatment group were all decreased compared with the model group(P<0.05),but there were no significant differences in the expression levels of TIMP1 proteins and m RNA(P>0.05).Conclusion:MXD could promote collagen degradation and reverse pancreatic fibrosis in CP rats via a mechanism involve up-regulation of MMP13 expression.展开更多
文摘为了研究煤矸石混凝土在疲劳荷载作用下的损伤特性,制作了煤矸石质量取代率分别为0、20%、40%、60%、80%、100%的混凝土试件,循环荷载取0.3 f c0和0.5 f c0,分别在循环加载2500次、5000次、7500次、10000次后测试其抗压强度和相对动弹性模量。结果表明:不同质量取代率的煤矸石混凝土在循环加载次数较少时,可致密内部结构,抗压强度不但无损而且性能还有所提升,煤矸石的质量取代率越高,混凝土抗压强度越小,达到最大值所需的循环次数越少,循环10000次煤矸石质量取代率40%的混凝土抗压强度开始出现损伤,且疲劳荷载越大,损伤越大;不同质量取代率的煤矸石混凝土在循环加载初期相对动弹性模量下降较快,后期逐渐趋于稳定,疲劳荷载越大,煤矸石质量取代率越高,损伤越严重,加速了结构内部微裂隙的扩展以及新孔隙的产生。
基金supported by the Key Research Lab of State Administration of Traditional ChineseComprehensive Investment Subject Construction Project of Tianjin Medical University(2016-2020)the National Natural Science Foundation of China(No.81703846)
文摘Modified Da-chai-hu Decoction(MDD), a traditional Chinese medicinal formulation, which was empirically generated from Da-chai-hu decoction, has been utilized to treat severe acute pancreatitis(SAP) for decades. The aim of the present study was to explore its potential organprotective mechanism in SAP. In the present study, rat SAP model was induced by retrograde injection of 3.5% sodium taurocholate into the biliopancreatic duct, MDD(23.35 g/kg body weight, twelve times the clinical dose) were orally given at 2 h before and 10 h after injection. At 12 h after model induction, blood was taken from vena cava for analysis of amylase, diamine oxidase(DAO), pulmonary surfactant protein-A(SP-A), and C-reactive protein(CRP). Histopathological change of pancreas, ileum and lung was assayed by H&E staining, myeloperoxidase(MPO) activity were determinated using colorimetric assay, and the expressions of occludin and nuclear factor-κB(NF-κB) were detected by real-time RT-PCR and western blot, respectively. In addition, the tissue concentrations of tumor necrosis factor-α(TNF-α), interleukin-1β(IL-1β), and monocyte chemoattractant protein-1(MCP-1) were measured by enzyme-linked immunosorbent assay(ELISA). The results showed that in SAP rats, MDD significantly alleviated histopathological damage, depressed the MPO activity and the concentrations of TNF-α, IL-1β, and MCP-1 of pancreas, ileum and lung, and reduced the serum levels of amylase [(3283.4±585.5) U·L^(-1) vs(5626.4±795.1) U·L^(-1)], DAO[(1100.1±334.3) U·L^(-1) vs(1666.4±525.3) U·L^(-1)] and CRP [(7.6±1.2) μg·mL^(-1) vs(17.8±3.8) μg·mL^(-1)]. However, the serum SP-A concentration [(106.1±16.6) pg·mL^(-1) vs(90.1±14.9) pg·mL^(-1)] was elevated when treated SAP rats with MDD. Furthermore, MDD increased the occludin expression and reduced the NF-κB expression in pancreas, ileum and lung of SAP rats. Our findings suggested that MDD administration was an effective therapeutic approach for SAP treatment. It could up-regulate occludin expression to protect intercellular tight junction and down-regulate NF-κB expression to inhibit inflammatory reaction of pancreas, ileum and lung.
基金Supported by the Tianjin Science and Technology Project,China(No.13RCGFSY19300)
文摘Objective： To investigate the effect of combined application of Xuebijing Injection（血必净注射液, XBJ） and resolvin D1（RvD1） on survival rate and the underlying mechanisms in mice with sepsisinduced lung injury. Methods： The cecal ligation and puncture（CLP） method was used to develop a mouse sepsis model. Specific pathogen free male C57 BL/6 mice were randomly divided into 5 groups（n=20 each）： sham, CLP, CLP＋XBJ, CLP＋RvD1 and CLP＋XBJ＋RvD1. After surgery, mice in the CLP＋XBJ, CLP＋RvD1 and CLP＋XBJ＋RvD1 groups were given XBJ（25 μL/g body weight）, RvD1（10 ng/g body weight）, and their combination（the same dose of XBJ and RvD1）, respectively. In each group, 12 mice were used to observe 1-week survival rate, while the rest were executed at 12 h. Whole blood was collected for flow cytometric analysis of leukocyte adhesion molecules CD18, lung tissues were harvested for observing pathological changes, and testing the activity of myeloperoxidase（MPO） and the expression of intercellular cell adhesion molecule 1（ICAM-1）. Results： Compared with the CLP group, the histopathological damage of the lung tissues was mitigated, MPO activity was decreased in the CLP＋XBJ and CLP＋RvD1 groups（P〈0.05）. In addition, the 1-week survival rate was improved, proportion of CD18-expressing cells in whole blood and ICAM-1 protein expression in lung tissue were decreased in the CLP＋XBJ＋RvD1 group（P〈0.05 or P〈0.01）. Conclusion： XBJ together with RvD1 could effectively inhibit leukocyte adhesion, reduce lung injury, and improve the survival rate of mice with sepsis.
基金Supported by the National Natural Science Foundation of China(No.81102686)。
文摘Objective:To investigate the effect of Modified Xiaochaihu Decoction(MXD,加味小柴胡汤)on collagen degradation in rats with chronic pancreatitis(CP).Methods:Rats were injected dibutyltin dichloride(DBTC,7 mg/kg of body weight)into the right caudal vein to induce CP model.Thirty heallhy male Wistar rats were randomly divided into three groups by a random number table:the control,the model and the treatment groups.Rats of treatment group were administered MXD(10 g/kg of body weight)orally once daily starting from the day post-model establishment.Pancreatic tissues were harvested after 28-day feeding and fibrosis was evaluated by picro-sirius red staining.The contents of collagen typeⅠandⅢwere detected using enzymelinked immunosorbent assay(ELISA),the expression of matrix metalloproteinase 13(MMP13)and tissue inhibitor of metalloproteinase 1(TIMP1)was analyzed by Western blot and real-time polymerase chain reaction(PCR).Results:The fibrosis scoring of pancreatic tissues,the concentrations of collagen typeⅠandⅢ,the expression levels of MMP13 and TIMP1 proteins and mRNA in the model group were all increased compared with the control group(P<0.05).After treatment with MXD,the fibrosis scoring of pancreatic tissues,the concentrations of collagen typeⅠandⅢ,the expression levels of MMP13 proteins and m RNA in the teatment group were all decreased compared with the model group(P<0.05),but there were no significant differences in the expression levels of TIMP1 proteins and m RNA(P>0.05).Conclusion:MXD could promote collagen degradation and reverse pancreatic fibrosis in CP rats via a mechanism involve up-regulation of MMP13 expression.
