miR166作为重要的转录后调节因子,在植物的生长发育和对逆境胁迫的反应中扮演着重要的角色。利用PmiREN、Ensembl Plants数据库、MEGA-X、DNAMAN软件以及RNAfold web server、WebLogo和psRNA Target在线网站对小麦miR166(Tae-miR166)基...miR166作为重要的转录后调节因子,在植物的生长发育和对逆境胁迫的反应中扮演着重要的角色。利用PmiREN、Ensembl Plants数据库、MEGA-X、DNAMAN软件以及RNAfold web server、WebLogo和psRNA Target在线网站对小麦miR166(Tae-miR166)基因家族的进化特性和表达模式进行分析。在PmiREN数据库中搜索到19个Tae-miR166基因家族成员,染色体定位发现Tae-miR166成员定位于14条染色体上。序列比对发现Tae-miR166的成熟体序列高度保守。进化分析结果表明,Tae-miR166基因家族成员分别处于4个进化分支。靶基因预测结果表明,Tae-miR166的靶基因包括Ⅲ类同源异型结构域亮氨酸拉链蛋白、β半乳糖苷酶和钙依赖性蛋白激酶等。转录组数据分析表明Tae-miR166家族19个成员在小麦6个组织中都有表达,在籽粒和穗中表达量最高。荧光定量PCR结果表明,Tae-miR166家族15个成员在镉、干旱和低温胁迫处理后的表达模式存在差异,说明Tae-miR166基因家族在植株抵御非生物胁迫时发挥着重要作用。本研究为进一步阐明Tae-miR166基因家族的功能提供了理论依据。展开更多
以昆仑山提孜那甫河流域为研究区,利用中分辨率成像光谱仪(MODIS)提供的大气数据基于Iqbal Model C模型估算晴空大气透射率空间分布,并引入地形开阔度(SVF)和遥感地表反照率数据分别用于估算散射辐射地形阻挡以及反射辐射反照率系数空...以昆仑山提孜那甫河流域为研究区,利用中分辨率成像光谱仪(MODIS)提供的大气数据基于Iqbal Model C模型估算晴空大气透射率空间分布,并引入地形开阔度(SVF)和遥感地表反照率数据分别用于估算散射辐射地形阻挡以及反射辐射反照率系数空间分布,最后结合Kumar模型的直接辐射地形阻挡模拟过程,实现对Kumar模型的改进,改进后模型综合考虑了大气以及地形对太阳辐射的影响。利用改进后模型对研究区地表太阳辐射时空分布进行模拟和分析,基于地面气象站点观测数据对模拟结果进行验证。结果表明:模型估算值与站点观测值存在很好的一致性,相关系数R^(2)为0.96,平均绝对误差(MAE)为1.47 MJ/m^(2),平均绝对相对误差(MARE)为12.26%。春季、夏季以及秋季模型的模拟精度较高,冬季模型的模拟精度较低,可能的原因为冬季MODIS大气数据有所低估。展开更多
With the rapid development of information technology in contemporary times,the blended teaching mode that blends online and offline courses has become an international trend in higher education.Taking blended tourism ...With the rapid development of information technology in contemporary times,the blended teaching mode that blends online and offline courses has become an international trend in higher education.Taking blended tourism management courses at Chongqing Three Gorges University as an example,we explored the impact of such teaching reform on student satisfaction based on the SERVPERF model.Empirical analysis of 179 valid questionnaires revealed that five elements of the reform,namely,reliability,assurance,valuableness,responsiveness,and empathy,have a significant positive impact on students’learning satisfaction.Specifically,in the context of blended courses,factors such as a stable and reliable teaching environment,comprehensively guaranteed educational conditions,teaching content that highly aligns with students’demands and value expectations,prompt responses to students’needs and feedback,and empathetic consideration of students’perspectives are critical for enhancing student satisfaction.Based on these conclusions,we propose several strategies and methods for improving the effectiveness of blended teaching in the hope of propelling its continuous improvement and optimization,thus further elevating the quality of higher education.展开更多
文摘miR166作为重要的转录后调节因子,在植物的生长发育和对逆境胁迫的反应中扮演着重要的角色。利用PmiREN、Ensembl Plants数据库、MEGA-X、DNAMAN软件以及RNAfold web server、WebLogo和psRNA Target在线网站对小麦miR166(Tae-miR166)基因家族的进化特性和表达模式进行分析。在PmiREN数据库中搜索到19个Tae-miR166基因家族成员,染色体定位发现Tae-miR166成员定位于14条染色体上。序列比对发现Tae-miR166的成熟体序列高度保守。进化分析结果表明,Tae-miR166基因家族成员分别处于4个进化分支。靶基因预测结果表明,Tae-miR166的靶基因包括Ⅲ类同源异型结构域亮氨酸拉链蛋白、β半乳糖苷酶和钙依赖性蛋白激酶等。转录组数据分析表明Tae-miR166家族19个成员在小麦6个组织中都有表达,在籽粒和穗中表达量最高。荧光定量PCR结果表明,Tae-miR166家族15个成员在镉、干旱和低温胁迫处理后的表达模式存在差异,说明Tae-miR166基因家族在植株抵御非生物胁迫时发挥着重要作用。本研究为进一步阐明Tae-miR166基因家族的功能提供了理论依据。
文摘以昆仑山提孜那甫河流域为研究区,利用中分辨率成像光谱仪(MODIS)提供的大气数据基于Iqbal Model C模型估算晴空大气透射率空间分布,并引入地形开阔度(SVF)和遥感地表反照率数据分别用于估算散射辐射地形阻挡以及反射辐射反照率系数空间分布,最后结合Kumar模型的直接辐射地形阻挡模拟过程,实现对Kumar模型的改进,改进后模型综合考虑了大气以及地形对太阳辐射的影响。利用改进后模型对研究区地表太阳辐射时空分布进行模拟和分析,基于地面气象站点观测数据对模拟结果进行验证。结果表明:模型估算值与站点观测值存在很好的一致性,相关系数R^(2)为0.96,平均绝对误差(MAE)为1.47 MJ/m^(2),平均绝对相对误差(MARE)为12.26%。春季、夏季以及秋季模型的模拟精度较高,冬季模型的模拟精度较低,可能的原因为冬季MODIS大气数据有所低估。
基金funded by the 2021 Chongqing Three Gorges University Higher Education Reform Project“Research on the Improvement of Teaching Quality in Blended Courses for Tourism Management”(JGZC2146)the Science and Technology Research Plan Project of Chongqing Municipal Education Commission“Research on the Effectiveness and Intrinsic Mechanisms of Virtual Spokespersons in Tourism Marketing in the Context of Digital Economy”(KJQN202301240)the Project of Chengdu-Chongqing Research Center for Coordinated Development of Education and Economic Society“Research on the Implementation Effect of the‘Double Reduction’Policy in Ethnic Regions in Sichuan and Chongqing:Based on the Parents’Perspective”(CYJXF23022).
文摘With the rapid development of information technology in contemporary times,the blended teaching mode that blends online and offline courses has become an international trend in higher education.Taking blended tourism management courses at Chongqing Three Gorges University as an example,we explored the impact of such teaching reform on student satisfaction based on the SERVPERF model.Empirical analysis of 179 valid questionnaires revealed that five elements of the reform,namely,reliability,assurance,valuableness,responsiveness,and empathy,have a significant positive impact on students’learning satisfaction.Specifically,in the context of blended courses,factors such as a stable and reliable teaching environment,comprehensively guaranteed educational conditions,teaching content that highly aligns with students’demands and value expectations,prompt responses to students’needs and feedback,and empathetic consideration of students’perspectives are critical for enhancing student satisfaction.Based on these conclusions,we propose several strategies and methods for improving the effectiveness of blended teaching in the hope of propelling its continuous improvement and optimization,thus further elevating the quality of higher education.
