目的评价发散型体外冲击波应用于青少年游泳运动员冈上肌肌腱病的疗效及安全性。方法采用前瞻性随机对照研究方法,将16名经临床及核磁共振确诊为肩袖冈上肌肌腱病并排除冈上肌撕裂的青少年游泳运动员随机分配到对照组及发散型体外冲击...目的评价发散型体外冲击波应用于青少年游泳运动员冈上肌肌腱病的疗效及安全性。方法采用前瞻性随机对照研究方法,将16名经临床及核磁共振确诊为肩袖冈上肌肌腱病并排除冈上肌撕裂的青少年游泳运动员随机分配到对照组及发散型体外冲击波治疗组(r ESWT组),对照组7例采用冰敷、按摩、中频治疗仪治疗,r ESWT组9例采用冰敷、发散型体外冲击波治疗。在治疗前及随访期内评估运动员可视化疼痛评分(VAS),简明肩关节测试评分(SST),Constant评分,治疗后复查肩关节MRI评价冈上肌肌腱的肌腱病分级。采用独立样本t检验进行组间对照,P<0.05为差异具有统计学意义。结果 r ESWT组治疗后6周及12周时VAS评分显著低于对照组(6周:t=3.757,P<0.01;12周:t=3.982,P<0.01)。与治疗前相比,治疗后MRI复查未见冈上肌肌腱信号改变。未发生冲击波治疗相关并发症。结论发散型体外冲击波可以在短期内有效改善青少年游泳运动员冈上肌肌腱病的肩痛症状,远期效果尚不明确。展开更多
Brassica rapa L.is cultivated globally and consumed in many areas worldwide.Using the transgenic Agrobacterium-mediated transformation method,which is a reproducible and efficient technique,genes can be transferred in...Brassica rapa L.is cultivated globally and consumed in many areas worldwide.Using the transgenic Agrobacterium-mediated transformation method,which is a reproducible and efficient technique,genes can be transferred into various B.rapa species.This review summarizes the processes involved in Agrobacterium-mediated transformation of B.rapa,including surface seed sterilization,co-cultivation with A.tumefaciens,induction of callus/shoot/root formation,and confirmation of transgenic plants.In addition,factors such as the Agrobacterium strain,plant genotype,explant age,transformation efficiency of the hybrid or inbred line,and the concentrations of N6-benzyl amino purine and naphthalene acetic acid,are discussed.And this review shows clearly how to do it,what to do,and what not to do in the transgenic Agrobacterium-mediated in Brassica rapa.The information presented here lays the foundation for a simple and efficient method that resolves existing problems and improves overall transgenic B.rapa production,thereby benefiting both basic and applied research.展开更多
文摘目的评价发散型体外冲击波应用于青少年游泳运动员冈上肌肌腱病的疗效及安全性。方法采用前瞻性随机对照研究方法,将16名经临床及核磁共振确诊为肩袖冈上肌肌腱病并排除冈上肌撕裂的青少年游泳运动员随机分配到对照组及发散型体外冲击波治疗组(r ESWT组),对照组7例采用冰敷、按摩、中频治疗仪治疗,r ESWT组9例采用冰敷、发散型体外冲击波治疗。在治疗前及随访期内评估运动员可视化疼痛评分(VAS),简明肩关节测试评分(SST),Constant评分,治疗后复查肩关节MRI评价冈上肌肌腱的肌腱病分级。采用独立样本t检验进行组间对照,P<0.05为差异具有统计学意义。结果 r ESWT组治疗后6周及12周时VAS评分显著低于对照组(6周:t=3.757,P<0.01;12周:t=3.982,P<0.01)。与治疗前相比,治疗后MRI复查未见冈上肌肌腱信号改变。未发生冲击波治疗相关并发症。结论发散型体外冲击波可以在短期内有效改善青少年游泳运动员冈上肌肌腱病的肩痛症状,远期效果尚不明确。
基金supported by funding from the China Agricultural Research System(CARS-25-A-01)the National Key Research and Development Program of China(2017YFD0101802)
文摘Brassica rapa L.is cultivated globally and consumed in many areas worldwide.Using the transgenic Agrobacterium-mediated transformation method,which is a reproducible and efficient technique,genes can be transferred into various B.rapa species.This review summarizes the processes involved in Agrobacterium-mediated transformation of B.rapa,including surface seed sterilization,co-cultivation with A.tumefaciens,induction of callus/shoot/root formation,and confirmation of transgenic plants.In addition,factors such as the Agrobacterium strain,plant genotype,explant age,transformation efficiency of the hybrid or inbred line,and the concentrations of N6-benzyl amino purine and naphthalene acetic acid,are discussed.And this review shows clearly how to do it,what to do,and what not to do in the transgenic Agrobacterium-mediated in Brassica rapa.The information presented here lays the foundation for a simple and efficient method that resolves existing problems and improves overall transgenic B.rapa production,thereby benefiting both basic and applied research.
