OBJECTIVE To investigate whether electroacupuncture(EA)ameliorates abnormal trigeminal neuralgia(TN)orofacial pain and anxiety-like behavior by altering synaptic plasticity in the hippocampus CA1.METHODS A mouse infra...OBJECTIVE To investigate whether electroacupuncture(EA)ameliorates abnormal trigeminal neuralgia(TN)orofacial pain and anxiety-like behavior by altering synaptic plasticity in the hippocampus CA1.METHODS A mouse infraorbital nerve transection model(pTION)of neuropathic pain was established,and EA or sham EA was used to treat ipsilateral acu⁃puncture points(GV20-Baihui and ST7-Xia⁃guan).Golgi-Cox staining and transmission elec⁃tron microscopy(TEM)were administrated to observe the changes of synaptic plasticity in the hippocampus CA1.RESULTS Stable and persistent orofacial allodynia and anxiety-like behav⁃iors induced by pT-ION were related to changes in hippocampal synaptic plasticity.Golgi stain⁃ings showed a decrease in the density of dendritic spines,especially mushroom-type dendritic spines,in hippocampal CA1 neurons of pT-ION mice.TEM results showed that the density of synapses,membrane thickness of the postsynaptic density,and length of the synaptic active zone were decreased,whereas the width of the synaptic cleft was increased in pTION mice.EA attenu⁃ated pT-ION-induced orofacial allodynia and anx⁃iety-like behaviors and effectively reversed the abnormal changes in dendritic spines and syn⁃apse of the hippocampal CA1 region.CONCLU⁃SION EA modulates synaptic plasticity of hippo⁃campal CA1 neurons,and reduces abnormal oro⁃facial pain and anxiety-like behavior,providing evidence for a TN treatment strategy.展开更多
目的探索品质管理圈(Quality control circle,QCC)在中医执业医师考试实践技能带教中的应用。方法选取2019年9月-2019年12月在山东中医药大学附属医院转科的30名硕士研究生,成立品管圈小组,运用头脑风暴法确定此次QCC活动主题为"...目的探索品质管理圈(Quality control circle,QCC)在中医执业医师考试实践技能带教中的应用。方法选取2019年9月-2019年12月在山东中医药大学附属医院转科的30名硕士研究生,成立品管圈小组,运用头脑风暴法确定此次QCC活动主题为"如何提升硕士研究生中医执业医师资格考试实践技能",采用5W1H(when、where、who、why、what和how)的方式从带教教师、实习学生、教学管理、科室硬件4个方面归纳影响硕士研究生中医执业医师资格考试相关临床操作技能的主要因素并制定实施对策。从有形成果和无形成果2个方面评价QCC活动效果。结果实施QCC活动后,实习学生的临床操作技能考核成绩明显高于实施QCC活动前,差异有统计学意义(P<0.05)。圈员各项能力均有提高,临床操作技能考核总分与无形成果中责任心、自信心、积极性呈正相关。结论QCC活动应用于中医执业医师考试实践技能带教,可以增强学生学习的积极性和主动性,提高带教教师的综合能力和QCC知识的应用能力。展开更多
基金the National Natural Science Foundation of China(82001190)Natural Sci⁃ence Foundation of Shandong Province(ZR2021LZY016)+1 种基金Natural Science Foundation of Shandong Province(ZR2020MH348)Science and Technology Foundation of Shandong Traditional Chinese Medicine(2020Q035)。
文摘OBJECTIVE To investigate whether electroacupuncture(EA)ameliorates abnormal trigeminal neuralgia(TN)orofacial pain and anxiety-like behavior by altering synaptic plasticity in the hippocampus CA1.METHODS A mouse infraorbital nerve transection model(pTION)of neuropathic pain was established,and EA or sham EA was used to treat ipsilateral acu⁃puncture points(GV20-Baihui and ST7-Xia⁃guan).Golgi-Cox staining and transmission elec⁃tron microscopy(TEM)were administrated to observe the changes of synaptic plasticity in the hippocampus CA1.RESULTS Stable and persistent orofacial allodynia and anxiety-like behav⁃iors induced by pT-ION were related to changes in hippocampal synaptic plasticity.Golgi stain⁃ings showed a decrease in the density of dendritic spines,especially mushroom-type dendritic spines,in hippocampal CA1 neurons of pT-ION mice.TEM results showed that the density of synapses,membrane thickness of the postsynaptic density,and length of the synaptic active zone were decreased,whereas the width of the synaptic cleft was increased in pTION mice.EA attenu⁃ated pT-ION-induced orofacial allodynia and anx⁃iety-like behaviors and effectively reversed the abnormal changes in dendritic spines and syn⁃apse of the hippocampal CA1 region.CONCLU⁃SION EA modulates synaptic plasticity of hippo⁃campal CA1 neurons,and reduces abnormal oro⁃facial pain and anxiety-like behavior,providing evidence for a TN treatment strategy.
文摘目的探索品质管理圈(Quality control circle,QCC)在中医执业医师考试实践技能带教中的应用。方法选取2019年9月-2019年12月在山东中医药大学附属医院转科的30名硕士研究生,成立品管圈小组,运用头脑风暴法确定此次QCC活动主题为"如何提升硕士研究生中医执业医师资格考试实践技能",采用5W1H(when、where、who、why、what和how)的方式从带教教师、实习学生、教学管理、科室硬件4个方面归纳影响硕士研究生中医执业医师资格考试相关临床操作技能的主要因素并制定实施对策。从有形成果和无形成果2个方面评价QCC活动效果。结果实施QCC活动后,实习学生的临床操作技能考核成绩明显高于实施QCC活动前,差异有统计学意义(P<0.05)。圈员各项能力均有提高,临床操作技能考核总分与无形成果中责任心、自信心、积极性呈正相关。结论QCC活动应用于中医执业医师考试实践技能带教,可以增强学生学习的积极性和主动性,提高带教教师的综合能力和QCC知识的应用能力。
