目的:观察龙血素B(Loureirin B,LB)对体外培养人永生化角质形成细胞(Immortalized human keratinocytes,HaCaT)增殖和迁移的影响,探究LB是否促进创面愈合再上皮化过程。方法:噻唑蓝(MTT)法检测不同浓度LB对体外培养HaCaT细胞增殖的影响...目的:观察龙血素B(Loureirin B,LB)对体外培养人永生化角质形成细胞(Immortalized human keratinocytes,HaCaT)增殖和迁移的影响,探究LB是否促进创面愈合再上皮化过程。方法:噻唑蓝(MTT)法检测不同浓度LB对体外培养HaCaT细胞增殖的影响,细胞划痕实验分析LB对HaCaT细胞迁移的影响。结果:5、10、25、50μg/mlLB呈剂量依赖性抑制HaCaT细胞增殖(P<0.05),10、25μg/mlLB呈剂量依赖性抑制划痕愈合及HaCaT细胞迁移(P<0.01)。结论:LB具有显著抑制HaCaT细胞增殖和迁移的作用,提示LB可能抑制创面愈合再上皮化过程。展开更多
Owing to the significant reductions in streamflow and an increase in human activities in recent years,the quality of surface water in Weihe River continues to pose environmental health concerns.We utilized hydrochemis...Owing to the significant reductions in streamflow and an increase in human activities in recent years,the quality of surface water in Weihe River continues to pose environmental health concerns.We utilized hydrochemistry and nitrogen and oxygen isotopes to elucidate the status and identify sources of nitrate pollution in the south and north banks for three seasons(flood,dry,and mean-flow periods)in the Weihe River watershed.A Bayesian isotope mixing model was applied to estimate the contributions of four potential NO_(3)-sources to river pollution(manure and sewage,soil nitrogen,inorganic fertilizer,and nitrate in precipitation).The U.S.Environmental Protection Agency(USEPA)evaluation model was implemented to evaluate the health risks associated with nitrate pollution in the surface water.Nitrate pollution was most severe during the dry period because the river flow was small.Due to the influence of the topography and land use type of the Weihe River,the pollution in the main stream was greater than that of the tributaries,and the pollution of the south bank was greater than that of the north bank.During the flood and mean-flow periods,δ^(15)N and δ^(18)O were mainly distributed in the NH_(4)^(+) of the fertilizer and soil nitrogen.During the dry period,δ^(15)N and δ^(18)O were mainly distributed in domestic sewage and manure regions.According to the Stable Isotope Analysis in R(SIAR)model,manure and sewage were the major nitrate sources during the dry period(73%).However,a decrease in the contribution from domestic sewage and manure was observed during the flood period(45%)compared to the dry period,but with a significantly increased contribution from soil nitrogen(23%)and inorganic fertilizer(21%).The health risk value in the dry period was higher than that in the wet and mean flow periods,and children are more susceptible to nitrate pollution than adults.Therefore,reducing the discharge of domestic sewage and manure and improving the utilization rate of nitrogen fertilizers may be effective measures to improve water quality in the watershed.展开更多
文摘目的:观察龙血素B(Loureirin B,LB)对体外培养人永生化角质形成细胞(Immortalized human keratinocytes,HaCaT)增殖和迁移的影响,探究LB是否促进创面愈合再上皮化过程。方法:噻唑蓝(MTT)法检测不同浓度LB对体外培养HaCaT细胞增殖的影响,细胞划痕实验分析LB对HaCaT细胞迁移的影响。结果:5、10、25、50μg/mlLB呈剂量依赖性抑制HaCaT细胞增殖(P<0.05),10、25μg/mlLB呈剂量依赖性抑制划痕愈合及HaCaT细胞迁移(P<0.01)。结论:LB具有显著抑制HaCaT细胞增殖和迁移的作用,提示LB可能抑制创面愈合再上皮化过程。
基金supported by National Natural Science Foundation of China(Grant No.41601017)Young Talent fund of University Association for Science and Technology in Shaanxi,China(Grant No.20190702)。
文摘Owing to the significant reductions in streamflow and an increase in human activities in recent years,the quality of surface water in Weihe River continues to pose environmental health concerns.We utilized hydrochemistry and nitrogen and oxygen isotopes to elucidate the status and identify sources of nitrate pollution in the south and north banks for three seasons(flood,dry,and mean-flow periods)in the Weihe River watershed.A Bayesian isotope mixing model was applied to estimate the contributions of four potential NO_(3)-sources to river pollution(manure and sewage,soil nitrogen,inorganic fertilizer,and nitrate in precipitation).The U.S.Environmental Protection Agency(USEPA)evaluation model was implemented to evaluate the health risks associated with nitrate pollution in the surface water.Nitrate pollution was most severe during the dry period because the river flow was small.Due to the influence of the topography and land use type of the Weihe River,the pollution in the main stream was greater than that of the tributaries,and the pollution of the south bank was greater than that of the north bank.During the flood and mean-flow periods,δ^(15)N and δ^(18)O were mainly distributed in the NH_(4)^(+) of the fertilizer and soil nitrogen.During the dry period,δ^(15)N and δ^(18)O were mainly distributed in domestic sewage and manure regions.According to the Stable Isotope Analysis in R(SIAR)model,manure and sewage were the major nitrate sources during the dry period(73%).However,a decrease in the contribution from domestic sewage and manure was observed during the flood period(45%)compared to the dry period,but with a significantly increased contribution from soil nitrogen(23%)and inorganic fertilizer(21%).The health risk value in the dry period was higher than that in the wet and mean flow periods,and children are more susceptible to nitrate pollution than adults.Therefore,reducing the discharge of domestic sewage and manure and improving the utilization rate of nitrogen fertilizers may be effective measures to improve water quality in the watershed.
