目的:通过研究刺山柑果提取物(Capparis spinosa fruit extract,CS)对干燥皮肤的改善作用,来探讨CS在皮肤护理中的作用,同时阐述其对于皮肤丝聚蛋白通路的调控机理。方法:于干燥皮肤上,通过多功能皮肤探头、皮肤胶带取样,检测不同浓度C...目的:通过研究刺山柑果提取物(Capparis spinosa fruit extract,CS)对干燥皮肤的改善作用,来探讨CS在皮肤护理中的作用,同时阐述其对于皮肤丝聚蛋白通路的调控机理。方法:于干燥皮肤上,通过多功能皮肤探头、皮肤胶带取样,检测不同浓度CS即时使用和持续使用下,皮肤含水量、经表皮失水率(Trans-epidermal water loss,TEWL)、鳞屑占比的变化。同时于低湿度环境培养下的重组表皮模型上,采用免疫荧光染色、CCK-8染色、高效液相法,分析CS对于丝聚蛋白降解相关指标的影响。结果:5%CS在使用后4 h可显著提升皮肤含水量,改善皮肤鳞屑占比。使用后7 d,1%、5%CS也可显著提升皮肤含水量,降低TEWL,改善皮肤鳞屑占比。3%CS涂抹于低湿度处理下的重组表皮模型上,4 h时可提升半胱天冬酶14、博来霉素水解酶表达量,促进吡咯烷酮羧酸(Pyrrolidone carboxylic acid,PCA)形成。24 h时改善细胞活力和皮肤结构,恢复皮肤含水量。结论:CS可通过调控丝聚蛋白通路来改善皮肤干燥状态,短时促进丝聚蛋白降解形成PCA,长效改善皮肤结构完整性和屏障功能。展开更多
For improving the evaluative accuracy of thermal protective clothing performance,the estimated methods of thermal response of skin and the human body were comprehensively described and analyzed.This study reviewed the...For improving the evaluative accuracy of thermal protective clothing performance,the estimated methods of thermal response of skin and the human body were comprehensively described and analyzed.This study reviewed the one-dimensional(1D)and multi-dimensional heat transfer models of the skin heat transfer model,including the corresponding heat and moisture transfer.Further,it investigated the influence of moisture transfer in vivo heat transfer.Moreover,the thermo-physiological model with active regulation was analyzed,especially in the local extremes,such as fingers and toes.Additionally,future research trends are discussed in estimating thermal protective performance.In developing the thermal protective model,it is essential to consider the geometric structure,local heat thermoregulation of extremities,and mass transfer inside the skin.展开更多
基金Fundamental Research Funds for the Central Universities,China(No.2232022G-08)International Cooperation Fund of Science and Technology Commission of Shanghai M unicipality,China(No.21130750100)。
文摘For improving the evaluative accuracy of thermal protective clothing performance,the estimated methods of thermal response of skin and the human body were comprehensively described and analyzed.This study reviewed the one-dimensional(1D)and multi-dimensional heat transfer models of the skin heat transfer model,including the corresponding heat and moisture transfer.Further,it investigated the influence of moisture transfer in vivo heat transfer.Moreover,the thermo-physiological model with active regulation was analyzed,especially in the local extremes,such as fingers and toes.Additionally,future research trends are discussed in estimating thermal protective performance.In developing the thermal protective model,it is essential to consider the geometric structure,local heat thermoregulation of extremities,and mass transfer inside the skin.
