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吴茱萸次碱通过抑制TLR4/NF-κB信号通路保护大鼠心肌缺血/再灌注损伤 被引量:22
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作者 杨晶 张晓坚 胡长平 《中国药理学通报》 CAS CSCD 北大核心 2017年第12期1707-1712,共6页
目的研究吴茱萸次碱是否通过抑制Toll样受体4(TLR4)/NF-κB信号通路保护大鼠心肌缺血/再灌注(I/R)损伤,以及其作用是否与促进降钙素基因相关肽(CGRP)释放有关。方法 SD大鼠在I/R前10 min静脉注射吴茱萸次碱和capsazepine(给吴茱萸次碱前... 目的研究吴茱萸次碱是否通过抑制Toll样受体4(TLR4)/NF-κB信号通路保护大鼠心肌缺血/再灌注(I/R)损伤,以及其作用是否与促进降钙素基因相关肽(CGRP)释放有关。方法 SD大鼠在I/R前10 min静脉注射吴茱萸次碱和capsazepine(给吴茱萸次碱前2 min静脉注射),然后结扎左冠状动脉前降支缺血60 min,再灌注3 h。测定心肌梗死面积、血浆中CGRP浓度和血清中肌酸激酶的活性;RTPCR分析心肌组织TLR4 mRNA表达水平;免疫组织化学方法检测心肌组织TLR4和NF-κB蛋白表达水平。结果吴茱萸次碱(100、300μg·kg^(-1))能明显降低心肌梗死面积和血清肌酸激酶活性,增加血浆CGRP浓度(P<0.05)。与I/R组比较,预先给予吴茱萸次碱也能明显降低TLR4 mRNA和蛋白表达以及NF-κB蛋白表达(P<0.05)。这些作用可被预先给予选择性辣椒素受体拮抗剂capsazepine(1.5 mg·kg^(-1))所取消。结论吴茱萸次碱通过抑制TLR4/NF-κB信号通路保护心肌I/R损伤,此作用与促进CGRP释放有关。 展开更多
关键词 TOLL样受体4 降钙素基因相关肽 缺血/再灌注损伤 吴茱萸次碱 心脏 大鼠
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Temperature effect on aerobic denitrification and nitrification 被引量:11
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作者 XIE Shu guang zhang xiao jian WANG Zhan sheng 《Journal of Environmental Sciences》 SCIE EI CAS CSCD 2003年第5期669-673,共5页
Nitrogen loss without organic removal in biofilter was observed and its possible reason was explained. A lower hydraulic loading could improve aerobic denitrification rate. Aerobic denitrification was seriously affect... Nitrogen loss without organic removal in biofilter was observed and its possible reason was explained. A lower hydraulic loading could improve aerobic denitrification rate. Aerobic denitrification was seriously affected by low temperature(below 10℃). However, nitrification rate remained high when the temperature dropped from 15℃ to 5℃. It seemed the autotrophic biofilm in BAF could alleviate the adverse effect of low temperature. 展开更多
关键词 aerobic denitrification NITRIFICATION BIOFILTER temperature effect
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Inactivation of Resistant Mycobacteria mucogenicum in Water:Chlorine Resistance and Mechanism Analysis 被引量:2
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作者 CHEN Yu Qiao CHEN Chao +2 位作者 zhang xiao jian ZHENG Qi LIU Yuan Yuan 《Biomedical and Environmental Sciences》 SCIE CAS CSCD 2012年第2期230-237,共8页
Objective To better understand the mechanism of chlorine resistance of mycobacteria and evaluate the efficiency of various disinfection processes.Methods Inactivation experiments of one strain Mycobacteria mucogenicum... Objective To better understand the mechanism of chlorine resistance of mycobacteria and evaluate the efficiency of various disinfection processes.Methods Inactivation experiments of one strain Mycobacteria mucogenicum,isolated from a drinking water distribution system in South China were conducted with various chlorine disinfectants.Inactivation efficiency and disinfectant residual,as well as the formation of organic chloramines,were measured during the experiments.Results This strain of M.mucogenicum showed high resistance to chlorine.The CT values of 99.9% inactivation by free chlorine,monochloramine and chlorine dioxide were detected as 29.6±1.46,170±6.16,and 10.9±1.55 min(mg/L) respectively,indicating that chlorine dioxide exhibited significantly higher efficiency than free chlorine and monochloramine.It was also found that M.mucogenicum reacted with chlorine disinfectants more slowly than S.aureus,but consumed more chlorine disinfectants during longer time of contact.Lipid analysis of the cell construction revealed that 95.7% of cell membrane lipid of M.mucogenicum was composed of saturated long chain fatty acids.Saturated fatty acids were regarded as more stable and more hydrophilic which enabled the cell membrane to prevent the diffusion of chlorine.Conclusion It was concluded that different compositions of cell membrane might endow M.mucogenicum with a higher chlorine resistance. 展开更多
关键词 Chlorine inactivation Chlorine consumption Lipid assay MECHANISM M. mucogenicum
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