Gd^(3+)调节铜纳米团簇聚集诱导发光自组装用于检测痕量六偏磷酸钠

本实验以谷胱甘肽(GSH)为还原剂和稳定剂,制备得到铜纳米簇(CuNCs)。经透射电镜表征,显示其分散性良好,平均粒径为4 nm,红外光谱证明GSH与CuNCs形成共价键,表明CuNCs成功被GSH修饰。以得到的功能化铜纳米簇为荧光探针,Gd^(3+)与CuNCs表面羧基高效结合形成复合物并使CuNCs聚集诱导发光。六偏磷酸钠可与Gd^(3+)更稳定络合,使Gd^(3+)从CuNCs表面释放,实现荧光猝灭。基于此,建立了六偏磷酸钠的荧光开关检测方法。通过对Gd^(3+)浓度、pH以及反应时间的优化,确定了最佳实验条件。采用此方法对实际样品茶饮料中六偏磷酸钠的含量进行测定,在0.2~16μmol/L有良好的线性关系,测得其检出限为0.08μmol/L,加标回收率为97.7%~104.1%,相对标准偏差低于4.0%。该方法操作简单、灵敏度高、价格低廉,在六偏磷酸钠的检测中效果良好。

环网供电系统电力线载波通信信道特性分析

为提高供电的可靠性,现代中压配电系统中将越来越多地出现环网拓扑结构。首先依据信息节点的信道建模方法,建立了具有环网的某实际配电网络的信道传输模型。然后在此基础上,对其信道传输特性进行了分析,利用仿真计算重点分析了各种因素对电力线载波通信模型的信道传输特性的影响规律。最后通过实验验证了所得结论的正确性和有效性。所使用的研究方法与总结出的相关规律对含有环网结构的配电系统的电力线载波通信调制具有重要意义。

重庆市渝北区4500例居民中医体质分布规律

目的:研究重庆市渝北区4500例居民的中医体质分布规律。方法:选取2021年3-12月在重庆市渝北区中医院体检科接受健康体检的4500例居民。采用问卷调查法,编制量表对入组者体质进行分析。统计重庆市渝北区居民中医体质分布情况,体质兼夹情况。分析重庆市渝北区居民不同性别体质分布情况。结果:4500例居民中平和质2142例,占比为47.60%,偏颇体质2072例,占比为46.04%。在偏颇体质中占比最高的为气虚质,其次为湿热质,占比最低的是特禀质,其次是阴虚质。可见重庆市渝北区居民中医体质多为平和质,常见偏颇体质为气虚质、湿热质、痰湿质、阳虚质、血瘀质及气郁质。4500例居民中单一体质共3945例,占比为87.67%,体质兼夹共555例,占比为12.33%。体质兼夹中以2种兼夹体质最为常见,占比为5.31%,其次为3种兼夹体质,占比为3.38%,7种兼夹体质最少,占比为0.60%,可见重庆市渝北区居民体质情况中多见单一体质,兼夹体质以2种兼夹较为多见,>2种兼夹体质较少。平和质、湿热质、阴虚质占比中,男性占比高于女性;气虚质、阳虚质、痰湿质、血瘀质、特禀质及气郁质占比中,女性占比高于男性。结论:重庆市渝北区居民虚性体质较多,居民的中医体质与区域性、性别存在一定联系,依据中医体质的辨识可为重庆市渝北区居民进行体质分类,从而进行更具针对性的中医干预,以发挥出中医管理模式的优势,更好地服务社区居民。

雀啄灸对痛经大鼠施灸穴区及子宫温度、血流灌注和血清AVP、PGF_(2α)及TXB_(2)的影响

目的:观察雀啄灸神阙、关元对寒湿凝滞型原发性痛经(PD)大鼠扭体反应及评分,施灸穴区及子宫温度值,子宫血流灌注量,大鼠血清精氨酸血管加压素(AVP),前列腺素(PG)F_(2α)及血栓素(TX)B_(2)含量的影响,探讨雀啄灸治疗PD的可能机制.方法:将32只雌性未孕健康Wistar大鼠采用随机数字表法分为正常组、模型组、布洛芬组及雀啄灸组,每组8只.除正常组外,其余三组采用冷水浴结合苯甲酸雌二醇复合缩宫素注射的方法造模.雀啄灸组大鼠于造模第8天接受雀啄灸神阙和关元治疗,每次30 min,1次/日,连续3 d;布洛芬组大鼠于造模第8天接受布洛芬溶液(规格为10 mL,125 mg)0.8 mL灌服治疗,1次/日,连续3 d;正常组与模型组给予0.8 mL生理盐水灌胃,1次/日,连续3 d.第11天,各组大鼠腹腔注射缩宫素(2 U/只).观察各组大鼠20 min内扭体潜伏期及扭体评分,观测大鼠神阙、关元穴区及在体子宫的温度值及血流灌注量,检测大鼠血清中AVP、PGF_(2α)及TXB_(2)的含量.结果:雀啄灸组及布洛芬组扭体潜伏期较模型组明显延长(P<0.01),扭体评分显著降低(P<0.01);与布洛芬组比较,雀啄灸组扭体潜伏期延长(P<0.01),扭体评分降低(P<0.01);与正常组比较,模型组大鼠神阙、关元穴区及子宫温度值,以及子宫血流灌注量明显降低,血清PGF_(2α)、AVP及TXB_(2)含量明显升高(P<0.01);与模型组比较,布洛芬组、雀啄灸组大鼠神阙、关元穴区及子宫温度值,以及子宫血流灌注量均明显升高,血清PGF_(2α)、AVP及TXB_(2)的含量明显降低(P<0.05或P<0.01);与布洛芬组比较,雀啄灸组大鼠神阙、关元穴区及子宫温度值,以及子宫血流灌注量均明显升高(P<0.05),血清AVP及TXB_(2)含量明显降低(P<0.05),血清PGF_(2α)含量无统计学差异(P>0.05).结论:雀啄灸对寒湿凝滞型原发性痛经镇痛效果显著,其机制可能与提高灸治穴区、子宫温度值及子宫血流灌注量,降低血清PGF_(2α)、AVP及TXB_(2)含量有关.

基于cAMP-PKA信号通路调控TRPV1表达研究温和灸治疗原发性痛经大鼠作用机制

目的基于cAMP-PKA信号通路调控瞬时受体电位香草酸亚型1(TRPV1)表达研究温和灸“神阙”“关元”治疗原发性痛经(PD)大鼠的作用机制。方法将32只雌性未孕Wistar大鼠随机分为空白组、模型组、温和灸组和辣椒平组,每组8只。除空白组外,其余组采用腹腔注射苯甲酸雌二醇+冰水浴建立PD寒湿凝滞证大鼠模型。造模第1日开始干预,温和灸组选取“神阙”“关元”温和灸,20 min/次,辣椒平组皮下注射辣椒平2 mg/kg,每日1次,连续10 d。观察大鼠扭体行为并进行评分,ELISA检测子宫组织前列腺素F_(2α)(PGF_(2α))、环磷酸腺苷(c AMP)含量,免疫荧光染色检测子宫组织TRPV1表达,Western blot检测子宫组织蛋白激酶A(PKA)、p-PKA、TRPV1蛋白表达。结果与空白组比较,模型组大鼠扭体潜伏期减少,扭体评分升高(P<0.01);子宫组织PGF_(2α)、cAMP含量升高(P<0.01),TRPV1、p-PKA蛋白表达升高(P<0.01)。与模型组比较,温和灸组和辣椒平组大鼠扭体潜伏期增加,扭体评分降低(P<0.01);子宫组织PGF_(2α)、cAMP含量降低(P<0.01),TRPV1、p-PKA蛋白表达降低(P<0.05,P<0.01)。与温和灸组比较,辣椒平组大鼠扭体潜伏期增加,扭体评分降低(P<0.01);子宫组织PGF_(2α)、cAMP含量降低(P<0.05,P<0.01),TRPV1蛋白表达降低(P<0.05)。结论温和灸“神阙”“关元”对PD大鼠具有明显镇痛作用,其机制可能与调控子宫组织cAMP-PKA信号通路介导TRPV1蛋白表达有关。

临床医学专业基础医学整合课程实施与初步评价:以山西医科大学为例

山西医科大学基础医学整合课程方案为,9门基础医学课程整合为10个教学模块,期间穿插PBL和TBL案例讨论、临床观摩见习、翻转课堂教学及未能整合的其他学科教学。为了评估学生对整合课程的兴趣和内在动机、知识的掌握和应用情况,对149名学生、16名基础教师和10名临床带教教师进行无记名问卷调查。发现90%以上的学生愿意参与课程整合、PBL和TBL案例讨论,少于10%的学生不愿意参与,认为新课程体系知识不连贯;但随着年级增高,不愿意参与翻转课堂的学生比例从6.7%增加到95.0%。大多数基础和临床教师认为,新课程体系使学生对基础知识掌握扎实、其辨析能力增强;但没有提升学生的动手能力。后期教改中,针对课程衔接、自主学习内容的选择等细节,仍需改进。

Effects of ginger-partitioned moxibustion on the expression levels of PGF2α, E2, P, and mRNAs of PGF2αR and E2R in rats with primary dysmenorrhea due to cold-dampness stagnation

Objective To observe the effects of ginger-partitioned moxibustion at Shenque(CV8)and Guanyuan(CV4)on the expression levels of endocrine-related molecules and their receptors in rats with primary dysmenorrhea(PD)due to cold-dampness stagnation,thus to explore their analgesic mechanisms.Methods Thirty-two female Wistar rats were divided into a normal group,a model group,a ginger-partitioned moxibustion group,and a Western medicine group according to the random number table method,with 8 rats in each group.Except for rats in the normal group,all other rats were treated with oxytocin combined with ice-water bath to establish the rat models of PD due to cold-dampness stagnation.After successful modeling,rats in the normal group and the model group did not receive treatment;rats in the ginger-partitioned moxibustion group received treatments with ginger-partitioned moxibustion at Shenque(CV8)and Guanyuan(CV4);rats in the Western medicine group received ibuprofen by intragastric administration.The writhing response of rats was compared among groups,and the serum levels of prostaglandin F2α(PGF2α),estrogen(estradiol,E2),progesterone(P),and the mRNA expression of PGF2αand E2 receptors in the uterine tissues were detected.Results No writhing behavior was observed in the normal group;compared with the normal group,the serum PGF2αand E2 levels in the model group were increased(P<0.01),while the P level was decreased(P<0.01),and the mRNA expression levels of the uterine PGF2αand E2 receptors were increased(P<0.01,P<0.05).Compared with the model group,the writhing behavior latency was prolonged,and the writhing response score was decreased in the ginger-partitioned moxibustion group and the Western medicine group(P<0.01);the serum PGF2αand E2 levels in the ginger-partitioned moxibustion group and the Western medicine group were decreased,while the P level was increased(P<0.05 or P<0.01);the mRNA expression levels of the uterine PGF2αand E2 receptors in the ginger-partitioned moxibustion group and the Western medicine group were decreased(P<0.05).Compared with the Western medicine group,the ginger-partitioned moxibustion group showed a prolonged writhing behavior latency,reduced writhing response score(P<0.05),and decreased serum E2 level(P<0.05),while no statistical differences in the serum PGF2αand P levels,or the mRNA expression levels of uterine PGF2αand E2 receptors(P>0.05).Conclusion The analgesic effect of ginger-partitioned moxibustion on PD due to cold-dampness stagnation may be related to regulating the mRNA expression levels of PGF2αand E2 receptors in the uterine tissues.