In this paper,^(99m)Tc-TRODAT-1 Micro-SPECT (single-photon emission computed tomography) was used for imaging dopamine transporter (DAT) in retinas and to investigate the changes of DAT in retinas of guinea pigs with ...In this paper,^(99m)Tc-TRODAT-1 Micro-SPECT (single-photon emission computed tomography) was used for imaging dopamine transporter (DAT) in retinas and to investigate the changes of DAT in retinas of guinea pigs with form deprivation myopia.Pigmented guinea pigs aged 3 weeks were derided into form deprivation myopia (FDM) group (n=6) and normal control group (n=6).The test group wore translucent goggles randomly for 4 weeks, and both groups underwent biometric measurement (refraction and axial length) before and after the experiment. Micro-SPECT retinas imaging was performed at the 4^(th) week after injection of ^(99m)Tc-TRODAT-1.The retinas were clearly resolved in the images.The ratio of ^(99m)Tc-TRODAT-1 uptake in the myopic retinas (11.55±2.80) was 3.64±1.40 lower than that in the control eye (15.20±1.98),and 2.35±1.05 lower than that in the fellow eyes (13.90±2.04).The results showed that ^(99m)Tc-TRODAT-1 Micro-SPECT eye imaging can be used to trace the distribution and changes of DAT in retina,and DAT in the myopic retinas were lower than that in the normal control eyes and fellow eyes.Micro-SPECT may provide a new approach for further studies on the role of dopamine system in the experimental myopia.展开更多
OBJECTIVE: To identify the intervention mechanism of the effect of electroacupuncture on the expression of Ang/Tie-2 m RNA and protein in rats with acute cerebral infarction induced by middle cerebral artery occlusion...OBJECTIVE: To identify the intervention mechanism of the effect of electroacupuncture on the expression of Ang/Tie-2 m RNA and protein in rats with acute cerebral infarction induced by middle cerebral artery occlusion(MCAO).METHODS: Altogether 120 Wistar rats were subjected to MCAO by inserting a nylon filament, andthen divided into 3 groups: control group, injured group and electroacupuncture group. The injured and electroacupuncture groups were further divided into the following 7 subgroups according to the time after MCAO: 3, 6, 12, 24 h, 3, 7 and 12 d, with 8 rats in each subgroup. The electroacupuncture group was given electroacupuncture treatment at Shuigou(GV 26) instantly after operation. The rats were killed at different time points according to their groups, and then the expression levels of Ang/Tie-2 m RNA and protein were detected using Real-Time polymerase chain reaction and immunohistochemical staining.RESULTS: The m RNA and protein expression levels of Ang/Tie-2 in the electroacupuncture group were significant higher than that in the injured group.CONCLUSION: The results suggested that electroacupuncture could significantly regulate the expression of Ang/Tie-2 m RNA and protein in the rats with acute cerebral infarction induced by MCAO, and enhance angiogenesis after ischemic penumbra.展开更多
OBJECTIVE： To determine the clinical efficacy and safety of electro-scalp acupuncture in the treatment of patients with acute ischemic stroke. METHODS： Totally 74 patients with acute ischemic stroke were enrolled an...OBJECTIVE： To determine the clinical efficacy and safety of electro-scalp acupuncture in the treatment of patients with acute ischemic stroke. METHODS： Totally 74 patients with acute ischemic stroke were enrolled and divided into either body acupuncture （Control） or electro-scalp acupuncture （ESA） groups according to randomized controlled principle. The patients in the control group were given body acupuncture treatment once daily for 28 d, whereas except for the body acupuncture, electro-scalp acupuncture was additional treatment given to the ESA group. Neurological deficits, everyday motor function and muscle strength were evaluated at baseline and the 28th d by NIH Stroke Scale （NIHSS）, FugI-Meyer Assessment （FMA） and Modified Barthel Index Score （MBI）, respectively. RESULTS： There were not obvious between-group differences in the baseline efficacy parameters （NI- HSS, FMA and MBI） （all P 〉 0.05）, whereas signifi- cant between-group differences were found in post-treatment NIHSS, FMA-UE and MBI scores （all P 〈 0.05）. After acupuncture treatment, systematic within-group improvements were found in the two groups for any of the efficacy parameters assessed （all P 〈 0.01）, and the ESA group showed higher significant improvements in NIHSS, FMA-UE and MBI scores （all P 〈 0.05）. CONCLUSION： Electro-scalp acupuncture was efficacious in the treatment of acute ischemic stroke, which resulted in meaningful improvements in neurologic function, motor function and activities of daily living of patients.展开更多
The purpose of this study was to develop fully 3D image reconstruction techniques for pinhole SPECT imaging with our Micro-SPECT system.Our studies involve in the derivation of projection operators,analysis of the sam...The purpose of this study was to develop fully 3D image reconstruction techniques for pinhole SPECT imaging with our Micro-SPECT system.Our studies involve in the derivation of projection operators,analysis of the sampling characteristics of pinhole SPECT imaging in Radon space,development of effective geometric calibration method for system misalignment,and 3D image reconstruction development and implementation with quantitative degrading compensation for pinhole SPECT with both circular and helical scan.The performances of pinhole SPECT imaging were evaluated using computer simulations and experiments with the Ultra-Micro Hot-Spot phantom,Ultra-Micro Defrise phantom and small-animal imaging.The results from the computer simulations and phantom imaging experiments indicate that the statistically-based iterative algorithms with quantitative compensation provide overall image quality improvement,and the system resolution is significantly recovered for quantitative imaging.The helical pinhole SPECT improves the axial field-of-view (FOV) as compared with the standard pinhole SPECT with circular-orbit scan.The mouse bone imaging experiment shows that the helical pinhole SPECT imaging also provides decent high-resolution whole-body small animal imaging.In conclusion,we have successfully developed a set of valid fully 3D image reconstruction techniques for single-pinhole SPECT imaging.These techniques can be easily extended to multi-pinhole SPECT imaging.展开更多
文摘In this paper,^(99m)Tc-TRODAT-1 Micro-SPECT (single-photon emission computed tomography) was used for imaging dopamine transporter (DAT) in retinas and to investigate the changes of DAT in retinas of guinea pigs with form deprivation myopia.Pigmented guinea pigs aged 3 weeks were derided into form deprivation myopia (FDM) group (n=6) and normal control group (n=6).The test group wore translucent goggles randomly for 4 weeks, and both groups underwent biometric measurement (refraction and axial length) before and after the experiment. Micro-SPECT retinas imaging was performed at the 4^(th) week after injection of ^(99m)Tc-TRODAT-1.The retinas were clearly resolved in the images.The ratio of ^(99m)Tc-TRODAT-1 uptake in the myopic retinas (11.55±2.80) was 3.64±1.40 lower than that in the control eye (15.20±1.98),and 2.35±1.05 lower than that in the fellow eyes (13.90±2.04).The results showed that ^(99m)Tc-TRODAT-1 Micro-SPECT eye imaging can be used to trace the distribution and changes of DAT in retina,and DAT in the myopic retinas were lower than that in the normal control eyes and fellow eyes.Micro-SPECT may provide a new approach for further studies on the role of dopamine system in the experimental myopia.
基金Supported by Tianjin Natural Science Foundation of Key Projects:Acupuncture Adjustment Th17/Treg Balance Against Nerve Inflammation Damage(No.16JCZDJC36200)And the National Natural Science Foundation of China:Acupuncture Regulation VD Brain Nerve Inflammation and Peripheral Immune Suppression of the Effect and Mechanism(No.81473766)+1 种基金Based on ACE/ACE2 axis and VEGF-Dll4/Notch Pathway to Study the Molecular Mechanism of Acupuncture Intervention in the Establishment of Collateral Circulation of Cerebral Infarction(No.81674056)Molecular Mechanism of Neurotoxicity Induced by Acupuncture in Rats with Cerebral Infarction based on Shh and Wnt Signaling Pathway(No.81473765)
文摘OBJECTIVE: To identify the intervention mechanism of the effect of electroacupuncture on the expression of Ang/Tie-2 m RNA and protein in rats with acute cerebral infarction induced by middle cerebral artery occlusion(MCAO).METHODS: Altogether 120 Wistar rats were subjected to MCAO by inserting a nylon filament, andthen divided into 3 groups: control group, injured group and electroacupuncture group. The injured and electroacupuncture groups were further divided into the following 7 subgroups according to the time after MCAO: 3, 6, 12, 24 h, 3, 7 and 12 d, with 8 rats in each subgroup. The electroacupuncture group was given electroacupuncture treatment at Shuigou(GV 26) instantly after operation. The rats were killed at different time points according to their groups, and then the expression levels of Ang/Tie-2 m RNA and protein were detected using Real-Time polymerase chain reaction and immunohistochemical staining.RESULTS: The m RNA and protein expression levels of Ang/Tie-2 in the electroacupuncture group were significant higher than that in the injured group.CONCLUSION: The results suggested that electroacupuncture could significantly regulate the expression of Ang/Tie-2 m RNA and protein in the rats with acute cerebral infarction induced by MCAO, and enhance angiogenesis after ischemic penumbra.
基金Supported by National Natural Science Foundation of China:Effect and Mechanism of Acupuncture on Regulating Cerebral Nerve Inflammation and Peripheral Immune Suppression in VD(No.81473766)Study on the Effects of Acupuncture on Hippocampal Synaptic Plasticity and the Mechanism of"Cell Stress"Based on LINCS Network Integrated Public Database(No.81574049)+1 种基金Based on ACE/ACE2 axis and VEGF-Dll4/Notch Pathway to Study the Molecular Mechanism of Acupuncture Intervention in the Establishment of Collateral Circulation of Cerebral Infarction(No.81674056)Key Project of Natural Science Foundation of Tianjin City:Acupuncture Adjustment Th17/Treg Balance Against Nerve Inflammation damage(No.16JCZDJC36200)
文摘OBJECTIVE： To determine the clinical efficacy and safety of electro-scalp acupuncture in the treatment of patients with acute ischemic stroke. METHODS： Totally 74 patients with acute ischemic stroke were enrolled and divided into either body acupuncture （Control） or electro-scalp acupuncture （ESA） groups according to randomized controlled principle. The patients in the control group were given body acupuncture treatment once daily for 28 d, whereas except for the body acupuncture, electro-scalp acupuncture was additional treatment given to the ESA group. Neurological deficits, everyday motor function and muscle strength were evaluated at baseline and the 28th d by NIH Stroke Scale （NIHSS）, FugI-Meyer Assessment （FMA） and Modified Barthel Index Score （MBI）, respectively. RESULTS： There were not obvious between-group differences in the baseline efficacy parameters （NI- HSS, FMA and MBI） （all P 〉 0.05）, whereas signifi- cant between-group differences were found in post-treatment NIHSS, FMA-UE and MBI scores （all P 〈 0.05）. After acupuncture treatment, systematic within-group improvements were found in the two groups for any of the efficacy parameters assessed （all P 〈 0.01）, and the ESA group showed higher significant improvements in NIHSS, FMA-UE and MBI scores （all P 〈 0.05）. CONCLUSION： Electro-scalp acupuncture was efficacious in the treatment of acute ischemic stroke, which resulted in meaningful improvements in neurologic function, motor function and activities of daily living of patients.
基金supported in part by the National Natural Science Foundation of China (30570520 & 10875162)
文摘The purpose of this study was to develop fully 3D image reconstruction techniques for pinhole SPECT imaging with our Micro-SPECT system.Our studies involve in the derivation of projection operators,analysis of the sampling characteristics of pinhole SPECT imaging in Radon space,development of effective geometric calibration method for system misalignment,and 3D image reconstruction development and implementation with quantitative degrading compensation for pinhole SPECT with both circular and helical scan.The performances of pinhole SPECT imaging were evaluated using computer simulations and experiments with the Ultra-Micro Hot-Spot phantom,Ultra-Micro Defrise phantom and small-animal imaging.The results from the computer simulations and phantom imaging experiments indicate that the statistically-based iterative algorithms with quantitative compensation provide overall image quality improvement,and the system resolution is significantly recovered for quantitative imaging.The helical pinhole SPECT improves the axial field-of-view (FOV) as compared with the standard pinhole SPECT with circular-orbit scan.The mouse bone imaging experiment shows that the helical pinhole SPECT imaging also provides decent high-resolution whole-body small animal imaging.In conclusion,we have successfully developed a set of valid fully 3D image reconstruction techniques for single-pinhole SPECT imaging.These techniques can be easily extended to multi-pinhole SPECT imaging.
