耦合大涡模拟(Large-Eddy Simulation,LES)与致动线方法(Actuator Line Method,ALM),对均匀入流下的水平轴风力机作数值模拟研究,探究三种亚格子(Subgrid-Scale,SGS)模型下风力机的尾流特性。结果表明:雷诺应力与湍流切应力在叶尖涡与...耦合大涡模拟(Large-Eddy Simulation,LES)与致动线方法(Actuator Line Method,ALM),对均匀入流下的水平轴风力机作数值模拟研究,探究三种亚格子(Subgrid-Scale,SGS)模型下风力机的尾流特性。结果表明:雷诺应力与湍流切应力在叶尖涡与叶根涡区有明显的最大值与极大值,尾迹随下游位置的变化呈现出逐渐增大的各向同性;平均速度亏损比产生的湍流更持久。不同SGS模型在相同叶尖速比下得到的轴向速度干扰因子和速度环量沿叶片径向分布几乎相同。相同位置处的尾涡由不同强度的涡旋组成,能量较小的涡旋是尾涡组成中的主要部分;尾流发展的过程中,强度较大的涡集中在尾涡中心,强度较小的涡在尾涡外围分布。对于所使用的三种亚格子模型,能预测出相似的尾流效应,亚格子模型的选择对尾流的模拟影响较小;νSGS=0时,求解过程只有数值耗散,仍能获得很好的模拟结果。展开更多
Objective:To investigate the effect of Xuduan Zhongzi prescription on the expression of HSP60 and HSP90 in testicular spermatogenic cells and semen quality of oligozoospermia and asthenospermia model rats.Methods:A to...Objective:To investigate the effect of Xuduan Zhongzi prescription on the expression of HSP60 and HSP90 in testicular spermatogenic cells and semen quality of oligozoospermia and asthenospermia model rats.Methods:A total of 40 SPF male rats were randomly divided into 10 blank control group and 30 model group.After successful modeling,the model group was divided into model control group,levocarnitine group and Xuduan Zhongzi prescription group with 10 rats in each group.The rats in levocarnitine group and Xuduan Zhongzi prescription group were given corresponding drugs by gavage according to the equivalent measurement of humanbody,the blank control group and model control group were given the same amount of normal saline by gavage,and the rats in each group were killed after 8 weeks of continuous administration.Take the left epididymis of each group for sperm quality detection,take the left testis of each group,observe the histomorphological changes by HE staining,and detect the expression of HSP60 and HSP90 in testicular tissue by immunohistochemistry and Western blot.Results:HE staining showed that compared with the blank control group,the convoluted tubules in the model group were significantly atrophic,degenerated and irregularly arranged,the spermatogenic epithelium showed a large number of vacuoles,Sertoli cell degeneration,obvious inflammatory infiltration in stromal cells,stromal cell proliferation,scattered rows of epithelial cells,a large number of exfoliation and degeneration,and sperm were rare.After the intervention of Xuduan Zhongzi prescription,the above lesions were significantly improved,the structure of seminiferous tubules was relatively regular and orderly,denser than that of the model group,sertoli cells and stromal cells increased significantly,the seminiferous epithelium was thicker than that of the model group,with rich layers,and a large number of sperm could be seen in the lumen.Immunohistochemistry and Western Blot showed that the expression of HSP60 and HSP90 increased significantly after the intervention of Xuduan Zhongzi prescription in model rats(P<0.01).The sperm quality test results showed that compared with the blank control group,the sperm concentration and activity rate in the model group decreased significantly,(P<0.01).After continuous seed cutting,the sperm concentration and activity rate of model rats were significantly increased,(P<0.01).Conclusion:Xuduan Zhongzi prescription may inhibit the apoptosis of testicular spermatogenic cells in oligoasthenospermia model rats by increasing the expression of HSP60 and HSP90,so as to improve sperm quality.展开更多
文摘耦合大涡模拟(Large-Eddy Simulation,LES)与致动线方法(Actuator Line Method,ALM),对均匀入流下的水平轴风力机作数值模拟研究,探究三种亚格子(Subgrid-Scale,SGS)模型下风力机的尾流特性。结果表明:雷诺应力与湍流切应力在叶尖涡与叶根涡区有明显的最大值与极大值,尾迹随下游位置的变化呈现出逐渐增大的各向同性;平均速度亏损比产生的湍流更持久。不同SGS模型在相同叶尖速比下得到的轴向速度干扰因子和速度环量沿叶片径向分布几乎相同。相同位置处的尾涡由不同强度的涡旋组成,能量较小的涡旋是尾涡组成中的主要部分;尾流发展的过程中,强度较大的涡集中在尾涡中心,强度较小的涡在尾涡外围分布。对于所使用的三种亚格子模型,能预测出相似的尾流效应,亚格子模型的选择对尾流的模拟影响较小;νSGS=0时,求解过程只有数值耗散,仍能获得很好的模拟结果。
基金Central Special Fund for Guiding Local Science and Technology Development(No.Guike ZY20198013)。
文摘Objective:To investigate the effect of Xuduan Zhongzi prescription on the expression of HSP60 and HSP90 in testicular spermatogenic cells and semen quality of oligozoospermia and asthenospermia model rats.Methods:A total of 40 SPF male rats were randomly divided into 10 blank control group and 30 model group.After successful modeling,the model group was divided into model control group,levocarnitine group and Xuduan Zhongzi prescription group with 10 rats in each group.The rats in levocarnitine group and Xuduan Zhongzi prescription group were given corresponding drugs by gavage according to the equivalent measurement of humanbody,the blank control group and model control group were given the same amount of normal saline by gavage,and the rats in each group were killed after 8 weeks of continuous administration.Take the left epididymis of each group for sperm quality detection,take the left testis of each group,observe the histomorphological changes by HE staining,and detect the expression of HSP60 and HSP90 in testicular tissue by immunohistochemistry and Western blot.Results:HE staining showed that compared with the blank control group,the convoluted tubules in the model group were significantly atrophic,degenerated and irregularly arranged,the spermatogenic epithelium showed a large number of vacuoles,Sertoli cell degeneration,obvious inflammatory infiltration in stromal cells,stromal cell proliferation,scattered rows of epithelial cells,a large number of exfoliation and degeneration,and sperm were rare.After the intervention of Xuduan Zhongzi prescription,the above lesions were significantly improved,the structure of seminiferous tubules was relatively regular and orderly,denser than that of the model group,sertoli cells and stromal cells increased significantly,the seminiferous epithelium was thicker than that of the model group,with rich layers,and a large number of sperm could be seen in the lumen.Immunohistochemistry and Western Blot showed that the expression of HSP60 and HSP90 increased significantly after the intervention of Xuduan Zhongzi prescription in model rats(P<0.01).The sperm quality test results showed that compared with the blank control group,the sperm concentration and activity rate in the model group decreased significantly,(P<0.01).After continuous seed cutting,the sperm concentration and activity rate of model rats were significantly increased,(P<0.01).Conclusion:Xuduan Zhongzi prescription may inhibit the apoptosis of testicular spermatogenic cells in oligoasthenospermia model rats by increasing the expression of HSP60 and HSP90,so as to improve sperm quality.
