辅酶Q10联合金凤丸对高龄妊娠女性卵巢功能和IVF-ET结局的影响

目的观察辅酶Q10联合金凤丸对高龄妊娠女性卵巢功能和体外受精-胚胎移植术(in vitro fertilization and embryo transfer,IVF-ET)结局的影响。方法回顾性分析2015年4月至2018年1月邯郸市中心医院收治的98例行IVF-ET的高龄妊娠女性患者的临床资料,将IVF-ET前予以辅酶Q10治疗者纳入对照组(38例),IVF-ET前予以辅酶Q10联合金凤丸治疗者纳入观察组(60例),并选取同期本院60例健康体检女性纳入空白对照组,比较对照组和观察组患者IVF-ET结局及其治疗前后性激素指标[雌二醇(estradiol,E2)、卵泡刺激素(follicle-stimulating hormone,FSH)、黄体生成素(luteinizing hormone,LH)水平及FSH/LH比值]、卵巢储备功能指标[抑制素B(inhibin B,INHB)、抗米勒管激素(anti-Müllerian hormone,AMH)]、卵巢间质动脉血流指标[阻力指数(resistance index,RI)、收缩期峰值流速(peak systolic velocity,PSV)]、平均卵巢直径(mean ovarian diameter,MOD)、窦状卵泡数(antral follicle count,AFC),并分别与空白对照组女性的指标水平进行比较。结果治疗后,观察组和对照组患者E2、LH、INHB、AMH水平均显著高于本组治疗前(均P<0.05),PSV、MOD均显著大于本组治疗前(均P<0.05),AFC均显著多于本组治疗前(均P<0.05),FSH水平均显著低于本组治疗前(均P<0.05)、FSH/LH比值和RI均显著小于本组治疗前(均P<0.05);且观察组患者上述指标变化幅度均显著大于对照组(均P<0.05)。观察组患者FSH和LH水平、FSH/LH比值、RI、PSV、MOD、AFC与空白对照组比较差异均无统计学意义(均P>0.05)。观察组患者获卵数显著多于对照组(P<0.05),胚胎种植率和临床妊娠率均显著高于对照组(均P<0.05)。结论对行IVF-ET治疗的高龄妊娠女性患者予以辅酶Q10联合金凤丸治疗,可以促进其卵巢功能恢复,改善IVF-ET结局。

Chronic oleoylethanolamide treatment attenuates diabetes-induced mice encephalopathyby activating peroxisome proliferator-activatedreceptor alpha in hippocampus

OBJECTIVE Oleoylethanolamide(OEA) is an endogenous peroxisome proliferatoractivated receptor alpha(PPARα) agonist that acts on the peripheral control of energy metabolism.Previous studies have shown that OEA exerts neuroprotection after cerebral ischemia.However,whether OEA affects the outcomes of diabetes-induced encephalopathy(DE) requires further study.METHODS The chronic effects of OEA on DE were evaluated in C57BL/6 and PPARαknockout mice,individually.The cognitive function was assessed with Morris water maze.The expression of receptor for advanced glycation end products(RAGE) and phosphorylation of Tau in mice hippocampus were determined using Western blotting.The influence of OEA in neuron loss and neuroplasticity were assessed with immunofluorescent staining and Western blotting.RESULTS OEA markedly ameliorated performance in the Morris water maze,which was correlated with its capabilities of suppressing glycometabolism and phosphorylation of Tau in the hippocampus.OEA offered protection from diabetes-induced impairments in hippocampal neuroplasticity.Furthermore,the changes in Morris water maze performance and neuron loss could not be observed in PPARα knockout mouse models with OEA administration.CONCLUSION The ability of OEA to control PPARα signaling can serve as a novel neuroprotective approach for the treatment of diabetes-induced encephalopathy.

Oleoylethanolamide protects against isch-emic stroke by modulating microglia M1/M2 polarization in PPARα-dependent manner

OBJECTIVE Oleoylethanolamide(OEA) has shown neuroprotective effect in treating acute and chronic ischemic stroke.However,it is unclear whether OEA is able to modulate microglia/macrophage polarization,which has recently been documented to be important in the pathology of ischemic stroke.This study explored the potential role of OEA in modulating the microglial phenotypes.METHODS In vivo,middle cerebral artery occlusion(MCAO) was induced in both PPARα-/-(KO) and wild-type(WT)mice.In vitro,primary cortical microglia or neuron or coculture from KO/WT mice was subjected to oxygen glucose deprivation(OGD).Western blotting and immunofluorescence were used for detecting the specialized protein expression of M1/M2,such as CD206 and CD16/32.q PCR was utilized to detect the signature gene change of M1/M2.RESULTS OEA significantly reduced neuron damage of mice after MCAO.More importantly,OEA promoted microglia/macrophage transferring from inflammatory M1 phenotype to a protective,anti-inflammatory M2 phenotype in vivo or in vitro.Interestingly,these benifical effects of OEA could not be observed in the KO mice or KO microglia.CONCLUSION Our results reveal a novel pharmacological effect of OEA in modulating microglia/macrophage polarization after MCAO,thus depening our understanding of neuroprotective mechanisms of OEA in treatment of ischemic stroke.Furthermore,this new mechanism may allow OEA to be used in many other microglia/macrophage polarizationrelated inflammatory diseases.

辅酶Q10联合金凤丸对体外授精-胚胎移植患者卵巢功能及子宫内膜容受性的影响

目的:探讨辅酶Q10联合金凤丸对体外授精-胚胎移植患者卵巢功能及子宫内膜容受性的影响。方法:选择2017年7月~2017年10月接诊的185例体外授精-胚胎移植患者进行研究,通过随机数表法将其分为观察组(n=95)和对照组(n=90)。对照组采用金凤丸进行治疗,观察组在对照组的基础上加用辅酶Q10进行治疗。治疗后,比较两组血清卵泡刺激素(FSH)、促黄体生成素(LH)、睾酮(T)、雌二醇(E2)、胰岛素(INS)、子宫内膜厚度、孕激素(P)水平、获卵数、受精率及妊娠率。结果:治疗后,两组患者血清FSH、LH、T、E2、INS水平均较治疗前明显下降,且观察组以上指标水平均显著低于对照组(P<0.05);两组患者子宫内膜厚度和血清P水平均较治疗前明显升高,且观察组以上指标均显著高于对照组(P<0.05);两组患者获卵数无明显差异,观察组患者受精率、妊娠率均显著高于对照组(P<0.05)。结论:辅酶Q10联合金凤丸可明显增加子宫内膜厚度,提高妊娠率。