目的评价早期预防脑瘫患儿继发髋关节发育不良疗法的有效性。方法检索CNKI、万方、VIP、CBM、PubMed、Embase、Web of Science和Cochrane Library数据库,收集从建库至2022年4月发表的有关预防脑瘫髋关节发育异常疗法的RCT。由两位评价...目的评价早期预防脑瘫患儿继发髋关节发育不良疗法的有效性。方法检索CNKI、万方、VIP、CBM、PubMed、Embase、Web of Science和Cochrane Library数据库,收集从建库至2022年4月发表的有关预防脑瘫髋关节发育异常疗法的RCT。由两位评价员独立筛选文献、提取资料并评价纳入研究的偏倚风险后,采用Stata 16.0软件和ADDIS 1.16.8软件进行贝叶斯网状Meta分析。结果最终纳入21个RCT,共包括1228例脑瘫患儿。网状Meta分析表明:(1)常规康复治疗联合家庭髋关节姿势管理、肌肉电刺激、穴位埋线、WBV,分别在改善MP值、内收肌肌张力、GMFM-E区评分、髋关节ROM评价方面疗效最佳;(2)在改善腘绳肌肌张力的评价中,常规康复治疗+靳三针头针+体针治疗效果明显优于常规康复治疗。结论家庭髋关节姿势管理在改善MP值方面临床前景最好,针灸联合常规康复治疗在改善肌张力方面疗效明确。展开更多
African swine fever virus(ASFV)is a lethal pathogen that causes severe threats to the global swine industry and it has already had catastrophic socio-economic effects.To date,no licensed prophylactic vaccine exists.Li...African swine fever virus(ASFV)is a lethal pathogen that causes severe threats to the global swine industry and it has already had catastrophic socio-economic effects.To date,no licensed prophylactic vaccine exists.Limited knowledge exists about the major immunogens of ASFV and the epitope mapping of the key antigens.As such,there is a considerable requirement to understand the functional monoclonal antibodies(mAbs)and the epitope mapping may be of utmost importance in our understanding of immune responses and designing improved vaccines,therapeutics,and diagnostics.In this study,we generated an ASFV antibody phage-display library from ASFV convalescent swine PBMCs,further screened a specific ASFV major capsid protein(p72)single-chain antibody and fused with an IgG Fc fragment(scFv-83-Fc),which is a specific recognition antibody against ASFV Pig/HLJ/2018 strain.Using the scFv-83-Fc mAb,we selected a conserved epitope peptide(221MTGYKH226)of p72 retrieved from a phage-displayed random peptide library.Moreover,flow cytometry and cell uptake experiments demonstrated that the epitope peptide can significantly promote BMDCs maturation in vitro and could be effectively uptaken by DCs,which indicated its potential application in vaccine and diagnostic reagent development.Overall,this study provided a valuable platform for identifying targets for ASFV vaccine development,as well as to facilitate the optimization design of subunit vaccine and diagnostic reagents.展开更多
为深入了解多孔碳在吸附领域研究的现状和前沿动态,以Web of Science(WoS)中Science Citation Index Expanded(SCI-E)引文数据库和CNKI数据库为数据源,使用Bibliometrix、Bibliometric平台和VOSviewer软件等对相关文献进行定量分析.结...为深入了解多孔碳在吸附领域研究的现状和前沿动态,以Web of Science(WoS)中Science Citation Index Expanded(SCI-E)引文数据库和CNKI数据库为数据源,使用Bibliometrix、Bibliometric平台和VOSviewer软件等对相关文献进行定量分析.结果表明,1997~2021年共发表SCI文献3566篇,文章数量总体呈逐年递增的趋势,近5a发文量上升速度较快,中国学术期刊共发表183篇文献;中国的发文总量及独立发文量均位居榜首,总量达1653篇,但平均被引频次相对较低;领域内主要研究力量分布在中国、美国、印度等地,且具有较大学术影响力的各作者间合作频繁.2010年至今温室气体中CO_(2)排放与多孔碳吸附议题之间有着十分紧密的学术联系,CO_(2)捕集将是碳中和课题下的一个持续研究热点.此外,开发和应用廉价、绿色、可持续的生物质资源制备性能优异的多孔碳材料是未来碳材料领域的持续目标之一,对于吸附机理的深入研究也将成为未来发展的方向之一.展开更多
基金supported by the National Natural Science Foundation of China(31941001 and 32002292)the Major Science and Technology Project of Henan Province,China(221100110600)the Natural Science Foundation of Henan Province(202300410199).
文摘African swine fever virus(ASFV)is a lethal pathogen that causes severe threats to the global swine industry and it has already had catastrophic socio-economic effects.To date,no licensed prophylactic vaccine exists.Limited knowledge exists about the major immunogens of ASFV and the epitope mapping of the key antigens.As such,there is a considerable requirement to understand the functional monoclonal antibodies(mAbs)and the epitope mapping may be of utmost importance in our understanding of immune responses and designing improved vaccines,therapeutics,and diagnostics.In this study,we generated an ASFV antibody phage-display library from ASFV convalescent swine PBMCs,further screened a specific ASFV major capsid protein(p72)single-chain antibody and fused with an IgG Fc fragment(scFv-83-Fc),which is a specific recognition antibody against ASFV Pig/HLJ/2018 strain.Using the scFv-83-Fc mAb,we selected a conserved epitope peptide(221MTGYKH226)of p72 retrieved from a phage-displayed random peptide library.Moreover,flow cytometry and cell uptake experiments demonstrated that the epitope peptide can significantly promote BMDCs maturation in vitro and could be effectively uptaken by DCs,which indicated its potential application in vaccine and diagnostic reagent development.Overall,this study provided a valuable platform for identifying targets for ASFV vaccine development,as well as to facilitate the optimization design of subunit vaccine and diagnostic reagents.
文摘为深入了解多孔碳在吸附领域研究的现状和前沿动态,以Web of Science(WoS)中Science Citation Index Expanded(SCI-E)引文数据库和CNKI数据库为数据源,使用Bibliometrix、Bibliometric平台和VOSviewer软件等对相关文献进行定量分析.结果表明,1997~2021年共发表SCI文献3566篇,文章数量总体呈逐年递增的趋势,近5a发文量上升速度较快,中国学术期刊共发表183篇文献;中国的发文总量及独立发文量均位居榜首,总量达1653篇,但平均被引频次相对较低;领域内主要研究力量分布在中国、美国、印度等地,且具有较大学术影响力的各作者间合作频繁.2010年至今温室气体中CO_(2)排放与多孔碳吸附议题之间有着十分紧密的学术联系,CO_(2)捕集将是碳中和课题下的一个持续研究热点.此外,开发和应用廉价、绿色、可持续的生物质资源制备性能优异的多孔碳材料是未来碳材料领域的持续目标之一,对于吸附机理的深入研究也将成为未来发展的方向之一.
