目的:检测孕晚期妇女B族链球菌(group B Streptococcus,GBS)的带菌情况及其耐药情况。方法:选取在南通大学附属妇幼保健院产科门诊进行产前检查的孕34~38周的孕妇2210例为研究对象。采集阴道内1/3处分泌物进行细菌培养与实时荧光聚合酶...目的:检测孕晚期妇女B族链球菌(group B Streptococcus,GBS)的带菌情况及其耐药情况。方法:选取在南通大学附属妇幼保健院产科门诊进行产前检查的孕34~38周的孕妇2210例为研究对象。采集阴道内1/3处分泌物进行细菌培养与实时荧光聚合酶链反应(real time fluorescent polymerase chain reaction,RT-PCR),观察2种方法的检测效果。再将GBS阳性者进行白带分析检测及耐药情况分析,同时选取GBS阴性患者作为对照。结果:2210例受检孕妇经细菌培养GBS阳性为8.28%,RT-PCR检测阳性为10.18%,RT-PCR检出率高于细菌培养。白带分析结果发现,GBS阳性与阴性标本中pH、白细胞酯酶、过氧化氢和唾液酸苷酶差异有统计学意义,而β葡萄糖醛酸酶和乙酰氨基葡萄糖苷酶差异无统计学意义。GBS对氨苄西林、利奈唑胺、青霉素G、奎奴普丁/达福普汀、替加环素、万古霉素的敏感率高(均为100.00%)。随访发现曾有患GBS阳性的患者,新生儿疾病患病率明显高于GBS阴性患者。结论:孕晚期GBS感染可提高不良妊娠结局的发生风险,导致新生儿疾病的增加,临床应注意筛查。展开更多
The peripheral nervous system is able to regenerate after injury, and regeneration is associated with the expression of many genes and proteins. MicroRNAs are evolutionarily conserved, small, non-coding RNA molecules ...The peripheral nervous system is able to regenerate after injury, and regeneration is associated with the expression of many genes and proteins. MicroRNAs are evolutionarily conserved, small, non-coding RNA molecules that regulate gene expression at the level of translation. In this paper, we focus on the identification and functional annotation of novel microRNAs in the proximal sciatic nerve after rat sciatic nerve transection. Using Solexa sequencing, computational analysis, and quantitative reverse transcription PCR verification, we identified 98 novel microRNAs expressed on days 0, 1, 4, 7, and 14 after nerve transection. Furthermore, we predicted the target genes of these microRNAs and analyzed the biological processes in which they were involved. The identified biological processes were consistent with the known time-frame of peripheral nerve injury and repair. Our data provide an important resource for further study of the role and regulation of microRNAs in peripheral nerve injury and regeneration.展开更多
文摘目的:检测孕晚期妇女B族链球菌(group B Streptococcus,GBS)的带菌情况及其耐药情况。方法:选取在南通大学附属妇幼保健院产科门诊进行产前检查的孕34~38周的孕妇2210例为研究对象。采集阴道内1/3处分泌物进行细菌培养与实时荧光聚合酶链反应(real time fluorescent polymerase chain reaction,RT-PCR),观察2种方法的检测效果。再将GBS阳性者进行白带分析检测及耐药情况分析,同时选取GBS阴性患者作为对照。结果:2210例受检孕妇经细菌培养GBS阳性为8.28%,RT-PCR检测阳性为10.18%,RT-PCR检出率高于细菌培养。白带分析结果发现,GBS阳性与阴性标本中pH、白细胞酯酶、过氧化氢和唾液酸苷酶差异有统计学意义,而β葡萄糖醛酸酶和乙酰氨基葡萄糖苷酶差异无统计学意义。GBS对氨苄西林、利奈唑胺、青霉素G、奎奴普丁/达福普汀、替加环素、万古霉素的敏感率高(均为100.00%)。随访发现曾有患GBS阳性的患者,新生儿疾病患病率明显高于GBS阴性患者。结论:孕晚期GBS感染可提高不良妊娠结局的发生风险,导致新生儿疾病的增加,临床应注意筛查。
基金supported by the National High Technology Research and Development Program of China (Grant No. 2006AA02A128)the National Natural Science Foundation of China (Grant No. 30870811)+1 种基金the Jiangsu Provincial Natural Science Foundation (Grant No. BK2008010)a project funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD)
文摘The peripheral nervous system is able to regenerate after injury, and regeneration is associated with the expression of many genes and proteins. MicroRNAs are evolutionarily conserved, small, non-coding RNA molecules that regulate gene expression at the level of translation. In this paper, we focus on the identification and functional annotation of novel microRNAs in the proximal sciatic nerve after rat sciatic nerve transection. Using Solexa sequencing, computational analysis, and quantitative reverse transcription PCR verification, we identified 98 novel microRNAs expressed on days 0, 1, 4, 7, and 14 after nerve transection. Furthermore, we predicted the target genes of these microRNAs and analyzed the biological processes in which they were involved. The identified biological processes were consistent with the known time-frame of peripheral nerve injury and repair. Our data provide an important resource for further study of the role and regulation of microRNAs in peripheral nerve injury and regeneration.
