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靶向调控缝隙连接蛋白43对骨髓间充质干细胞成骨分化的影响 被引量:1
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作者 谢锡洪 张振伟 +1 位作者 林泽金 林利忠 《骨科》 CAS 2020年第2期149-154,共6页
目的探讨缝隙连接蛋白43(connexin 43,Cx43)对骨髓间充质干细胞(bone marrow mesen chymal stem cells,BMSCs)成骨分化的影响。方法将Cx43目的基因克隆后与慢病毒载体pHBLV CMVIE Puro进行连接,重组载体与包装载体共同转染293T细胞后包... 目的探讨缝隙连接蛋白43(connexin 43,Cx43)对骨髓间充质干细胞(bone marrow mesen chymal stem cells,BMSCs)成骨分化的影响。方法将Cx43目的基因克隆后与慢病毒载体pHBLV CMVIE Puro进行连接,重组载体与包装载体共同转染293T细胞后包装生产病毒。获取小鼠BMSCs,流式细胞术检测细胞表面标志。将慢病毒载体pHBLV Cx43 Puro(Cx43组)、pHBLV CMVIE Puro(空载体组)转染BMSCs,同时设立空白对照组;Western blot和qRT PCR检测三组细胞中Cx43的表达。成骨诱导培养基诱导三组细胞成骨分化,qRT PCR检测成骨相关基因Runt相关转录因子2(runt related transcrip tion factor 2,Runx2)、锌指结构转录因子(Osterix/Sp7)、转录活化因子4(activating transcription factor 4,ATF4)、骨唾蛋白(bone sialoprotein,BSP)的表达;碱性磷酸酶(alkaline phosphatase,ALP)活性检测试剂盒定量分析三组BMSCs的成骨分化能力。结果流式细胞术检查示BMSCs中CD29、CD44、CD90呈阳性表达,而CD14和CD34呈阴性表达,pHBLV Cx43 Puro转染BMSCs后Cx43蛋白和mRNA表达显著增加。成骨诱导后Cx43组成骨相关基因Runx2、Osterix/Sp7、ATF4和BSP的mRNA表达显著高于其他两组(P均<0.05),ALP活性明显高于其他两组(P<0.05)。结论Cx43高表达能够显著促进BMSCs向成骨细胞分化,其可能成为骨损伤修复的有效靶点。 展开更多
关键词 骨髓间充质干细胞 缝隙连接蛋白43 成骨分化 慢病毒
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Probing PM_(2.5) with terahertz wave 被引量:4
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作者 LI Qian ZHAO Kun +4 位作者 zhang Lei wei LIANG Chuan zhang zhen wei zhang Cun Lin HAN Dong Hai 《Science China(Physics,Mechanics & Astronomy)》 SCIE EI CAS 2014年第12期2354-2356,共3页
The goal of continuous ambient participate monitoring has been accomplished by the use of terahertz (THz) wave. The fre- quency-dependent spectrum and absorbance of the particulate matter (PM2.5) were measured in ... The goal of continuous ambient participate monitoring has been accomplished by the use of terahertz (THz) wave. The fre- quency-dependent spectrum and absorbance of the particulate matter (PM2.5) were measured in the range of 0-10 THz. The PM2.5 concentration ,o was calculated according to the sampling time and air flow. With the increase of ,o, the THz wave am- plitude gradually decreased and the absorbance A of PM2.5 increased. The relationship between p and A can be described mathematically through p∝ A0.5. Our results demonstrate that the terahertz wave could be a valuable tool to monitor and in- spect the PM2.5 concentration. 展开更多
关键词 PM2.5 TERAHERTZ CONCENTRATION ABSORBANCE
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