The property of starch in rice grain endosperm is a very important determinant for rice quality, and it is essential to understand the genetic effect of the genes related to starch synthesis in high-yielding rice vari...The property of starch in rice grain endosperm is a very important determinant for rice quality, and it is essential to understand the genetic effect of the genes related to starch synthesis in high-yielding rice varieties for rice quality improvement. The physicochemical properties （e.g., amylose content, gel consistency, and RVA profile） were assessed on 53 rice varieties, including certain typical indica/japonica landraces and certain high-yielding modern varieties. And molecular markers for Sbel, Sbe3 developed on the basis of sequence diversities between the rice subspecies indica and japonica, together with PCR-Acc I marker for Wx gene were used to investigate the genotypes of 53 rice cultivars. The result showed that the developed molecular markers for Wx, Sbel, Sbe3 could distinguish indica or japonica alleles at three loci. Among all the 53 rice cultivars, six genotypes were observed when Sbel, Sbe3, and Wx loci were considered together, while the genotypes of WxiSbelJSbe3i and WxiSbelJSbe3J were absent. In order to explore the genetic effects of the three genes, especially for starch branching enzyme genes, ANOVA and multiple comparison analysis were conducted. The results showed that rice cultivars with different genotypes exhibited different phenotypes, including amylose content, gel consistency and certain RVA characteristics, and the significant differences among the six genotypes were observed. It was concluded that these three genes had randomly recombined during the process of the rice variety development. And the genetic effects of indica and japonica alleles at three gene loci were different, of which, Wx gene plays a major role in determining the starch properties, followed by Sbel and Sbe3, and the genetic effects of Sbel and Sbe3 in different backgrounds （Wx~, WxJ） are different. The results have provided a clue for rice good quality variety development, and the molecular markers will benefit to the improvement in quality of rice.展开更多
为了解微生物菌剂对保护地草莓产量和效益的影响,通过滴灌施肥试验,验证了微生物菌剂、大量元素水溶肥及常规肥料在保护地草莓生产上的实际应用效果。结果表明,施用微生物菌剂进行滴灌施肥,在增产、节水、节肥、节药等方面综合节本增收...为了解微生物菌剂对保护地草莓产量和效益的影响,通过滴灌施肥试验,验证了微生物菌剂、大量元素水溶肥及常规肥料在保护地草莓生产上的实际应用效果。结果表明,施用微生物菌剂进行滴灌施肥,在增产、节水、节肥、节药等方面综合节本增收效果最为明显。较常规施肥增产5943 kg/hm^2,增产率17.33%,节水600 m 3/hm^2,节水率10.53%,节水270元/hm^2,节肥90元/hm^2,节肥率20.00%,节省病虫害防治投入975元/hm^2,合计节本增收60765.0元/hm^2。展开更多
基金This work was financially supported by the National Natural Science Foundation of China （30270809, 30300220 and 30530470）National 973 Project of China （2005CB 120804）.
文摘The property of starch in rice grain endosperm is a very important determinant for rice quality, and it is essential to understand the genetic effect of the genes related to starch synthesis in high-yielding rice varieties for rice quality improvement. The physicochemical properties （e.g., amylose content, gel consistency, and RVA profile） were assessed on 53 rice varieties, including certain typical indica/japonica landraces and certain high-yielding modern varieties. And molecular markers for Sbel, Sbe3 developed on the basis of sequence diversities between the rice subspecies indica and japonica, together with PCR-Acc I marker for Wx gene were used to investigate the genotypes of 53 rice cultivars. The result showed that the developed molecular markers for Wx, Sbel, Sbe3 could distinguish indica or japonica alleles at three loci. Among all the 53 rice cultivars, six genotypes were observed when Sbel, Sbe3, and Wx loci were considered together, while the genotypes of WxiSbelJSbe3i and WxiSbelJSbe3J were absent. In order to explore the genetic effects of the three genes, especially for starch branching enzyme genes, ANOVA and multiple comparison analysis were conducted. The results showed that rice cultivars with different genotypes exhibited different phenotypes, including amylose content, gel consistency and certain RVA characteristics, and the significant differences among the six genotypes were observed. It was concluded that these three genes had randomly recombined during the process of the rice variety development. And the genetic effects of indica and japonica alleles at three gene loci were different, of which, Wx gene plays a major role in determining the starch properties, followed by Sbel and Sbe3, and the genetic effects of Sbel and Sbe3 in different backgrounds （Wx~, WxJ） are different. The results have provided a clue for rice good quality variety development, and the molecular markers will benefit to the improvement in quality of rice.
文摘为了解微生物菌剂对保护地草莓产量和效益的影响,通过滴灌施肥试验,验证了微生物菌剂、大量元素水溶肥及常规肥料在保护地草莓生产上的实际应用效果。结果表明,施用微生物菌剂进行滴灌施肥,在增产、节水、节肥、节药等方面综合节本增收效果最为明显。较常规施肥增产5943 kg/hm^2,增产率17.33%,节水600 m 3/hm^2,节水率10.53%,节水270元/hm^2,节肥90元/hm^2,节肥率20.00%,节省病虫害防治投入975元/hm^2,合计节本增收60765.0元/hm^2。
