目的探讨miR-495在肝癌组织中的表达以及对肝癌MHCC-97H细胞的影响。方法选取2017年1月至2018年1月我院保存的肝癌组织标本56例(肝癌组),同时选取正常肝脏组织标本40例作为对照组,采用逆转录实时荧光定量PCR(qRT-PCR)检测miR-495表达;...目的探讨miR-495在肝癌组织中的表达以及对肝癌MHCC-97H细胞的影响。方法选取2017年1月至2018年1月我院保存的肝癌组织标本56例(肝癌组),同时选取正常肝脏组织标本40例作为对照组,采用逆转录实时荧光定量PCR(qRT-PCR)检测miR-495表达;选取肝癌MHCC-97H细胞,随机分为对照组、空白质粒组和转染组,其中空白质粒组转染空白质粒,转染组转染miR-495抑制剂,用qRT-PCR检测各组细胞miR-495表达,用CCK法检测各组细胞增殖活性,用Transwell细胞迁移实验检测各组细胞迁移能力。多组间数据比较使用方差分析,进一步两两比较采用LDS-t检验,两组比较使用t检验。结果肝癌组miR-495相对表达量为2.043±0.382,高于对照组,两组比较,差异有统计学意义(P<0.05);临床分期Ⅲ~Ⅳ期、有淋巴结转移患者miR-495相对表达量分别为2.265±0.284和2.290±0.355,高于Ⅰ~Ⅱ期、无淋巴结转移患者(P<0.05),差异有统计学意义;转染组miR-495相对表达量为0.653±0.102,低于对照组和空白质粒组(P<0.05),差异有统计学意义;转染组培养24 h和48 h MHCC-97H细胞A值分别为0.404±0.106和0.604±0.136,低于对照组和空白质粒组(P<0.05);转染组MHCC-97H细胞迁移数为(6.10±1.20)个,低于对照组和空白质粒组(P<0.05)。结论miR-495在肝癌组织中呈高表达,与临床病理特征有一定关系;miR-495对肝癌MHCC-97H细胞增殖以及迁移能力有一定影响。展开更多
Mesoporous titania-grafted poly(styrene-divinylbenzene)/maleic anhydride [P(St-DVB)/MA] nanocomposite microspheres were prepared by an open ring reaction method.The titania nanoparticles were first modified by attachm...Mesoporous titania-grafted poly(styrene-divinylbenzene)/maleic anhydride [P(St-DVB)/MA] nanocomposite microspheres were prepared by an open ring reaction method.The titania nanoparticles were first modified by attachment of amino groups to their surface to prevent particle aggregation,and to allow the nanoparticles to covalently bond the polymer microspheres,the surface of which was modified by attachment of MA functional groups to enable the polymer to retain their porous structures and to react with the amino groups on the surface of the titania particles.The porous nanocomposite microspheres were detected by FTIR,SEM,TEM,XRD and UV-Vis spectrophotometry.The results indicated that the nanocomposite microspheres were composed of nanosized titania uniformly distributed on the surface,and exhibited better UV absorbing property than pure polymer microspheres or unmodified titania.Furthermore,compared with pure porous polymer microspheres,the nanocomposite microspheres showed more efficient UV protection and slow release of Parsol-1789(a photo-reactive and cosmetic agent) held inside the porous network of the microspheres.展开更多
Owing to the rapidly increasing output of nano-scale titanium dioxide(TiO2) particles,their potential risk for central nerve system(CNS) has elicited much concern recently.Microglia is the resident macrophage in CNS a...Owing to the rapidly increasing output of nano-scale titanium dioxide(TiO2) particles,their potential risk for central nerve system(CNS) has elicited much concern recently.Microglia is the resident macrophage in CNS and essential for the homeostasis of the CNS microenvironment.They are supposed to response to nanoparticles depositing in the brain tissues.Therefore,we investigated the cytotoxic effects of TiO2 NPs on microglia N9 cells in vitro.Results of propidium iodide/fluorescein diacetate(PI/FDA) double staining and MTT test clearly showed that TiO2 NPs more efficiently affected the viability of microglia N9 cells.Further Hoechst 33258 staining and flow cytometric analysis proved that nano-scale but not normal scale TiO2 induced apoptosis in vitro.These data suggest that TiO2 NPs can elicit apoptosis of N9 cells in vitro and thus present a potential risk for CNS.展开更多
A novel vehicle for the delivery of aspirin （ASA） was prepared from porous nano-hydroxyapafite/poly（styrene-divinylbenzene） [nano-HAP/P（St-DVB）] composite microspheres by grafting nano-HAP [Ca10（PO4）6（OH）2] ...A novel vehicle for the delivery of aspirin （ASA） was prepared from porous nano-hydroxyapafite/poly（styrene-divinylbenzene） [nano-HAP/P（St-DVB）] composite microspheres by grafting nano-HAP [Ca10（PO4）6（OH）2] onto porous P（St-DVB） micro- spheres. Four types of porous composite microspheres were prepared, each with different nano-HAP contents. The ASA-loaded composite microspheres prepared with 10% and 15% nano-HAP （mass ratio） exhibited excellent buoyancy with relatively short instantaneous floating time （within l0 min） and a long sustained floating time （12 h） in simulated gastric juice. They also offered good sustained release of ASA （up to 8 h）. Furthermore, these composite microspheres displayed good buff- ering capacity that prevented the buildup of acidity caused by hydrolysis of ASA, keeping the pH of gastric juice within the normal range （pH 0.9 to 1.5）. The results showed that porous nano-HAP/P（St-DVB） composite microspheres prepared with 10% and 15% nano-HAP could be used as a novel drug carrier for ASA, providing a sustained release dose without leading to stomach irritation, a side effect that is often associated with ASA medication.展开更多
文摘目的探讨miR-495在肝癌组织中的表达以及对肝癌MHCC-97H细胞的影响。方法选取2017年1月至2018年1月我院保存的肝癌组织标本56例(肝癌组),同时选取正常肝脏组织标本40例作为对照组,采用逆转录实时荧光定量PCR(qRT-PCR)检测miR-495表达;选取肝癌MHCC-97H细胞,随机分为对照组、空白质粒组和转染组,其中空白质粒组转染空白质粒,转染组转染miR-495抑制剂,用qRT-PCR检测各组细胞miR-495表达,用CCK法检测各组细胞增殖活性,用Transwell细胞迁移实验检测各组细胞迁移能力。多组间数据比较使用方差分析,进一步两两比较采用LDS-t检验,两组比较使用t检验。结果肝癌组miR-495相对表达量为2.043±0.382,高于对照组,两组比较,差异有统计学意义(P<0.05);临床分期Ⅲ~Ⅳ期、有淋巴结转移患者miR-495相对表达量分别为2.265±0.284和2.290±0.355,高于Ⅰ~Ⅱ期、无淋巴结转移患者(P<0.05),差异有统计学意义;转染组miR-495相对表达量为0.653±0.102,低于对照组和空白质粒组(P<0.05),差异有统计学意义;转染组培养24 h和48 h MHCC-97H细胞A值分别为0.404±0.106和0.604±0.136,低于对照组和空白质粒组(P<0.05);转染组MHCC-97H细胞迁移数为(6.10±1.20)个,低于对照组和空白质粒组(P<0.05)。结论miR-495在肝癌组织中呈高表达,与临床病理特征有一定关系;miR-495对肝癌MHCC-97H细胞增殖以及迁移能力有一定影响。
基金supported by the Heilongjiang Province Department of Health (Grant No 2009-259)the Natural Science Foundation of Heilongjiang Province (Grant Nos ZD200807-01,ZD2008-08 and B200810)+1 种基金the National Natural Science Foundation of China (Grant No 3087007)the Educational Office of Heilongjiang Province (1154HZ11)
文摘Mesoporous titania-grafted poly(styrene-divinylbenzene)/maleic anhydride [P(St-DVB)/MA] nanocomposite microspheres were prepared by an open ring reaction method.The titania nanoparticles were first modified by attachment of amino groups to their surface to prevent particle aggregation,and to allow the nanoparticles to covalently bond the polymer microspheres,the surface of which was modified by attachment of MA functional groups to enable the polymer to retain their porous structures and to react with the amino groups on the surface of the titania particles.The porous nanocomposite microspheres were detected by FTIR,SEM,TEM,XRD and UV-Vis spectrophotometry.The results indicated that the nanocomposite microspheres were composed of nanosized titania uniformly distributed on the surface,and exhibited better UV absorbing property than pure polymer microspheres or unmodified titania.Furthermore,compared with pure porous polymer microspheres,the nanocomposite microspheres showed more efficient UV protection and slow release of Parsol-1789(a photo-reactive and cosmetic agent) held inside the porous network of the microspheres.
基金Supported by the German Academic Exchange Service (DAAD) and Chinese Scholarship Council (CSC)
文摘Owing to the rapidly increasing output of nano-scale titanium dioxide(TiO2) particles,their potential risk for central nerve system(CNS) has elicited much concern recently.Microglia is the resident macrophage in CNS and essential for the homeostasis of the CNS microenvironment.They are supposed to response to nanoparticles depositing in the brain tissues.Therefore,we investigated the cytotoxic effects of TiO2 NPs on microglia N9 cells in vitro.Results of propidium iodide/fluorescein diacetate(PI/FDA) double staining and MTT test clearly showed that TiO2 NPs more efficiently affected the viability of microglia N9 cells.Further Hoechst 33258 staining and flow cytometric analysis proved that nano-scale but not normal scale TiO2 induced apoptosis in vitro.These data suggest that TiO2 NPs can elicit apoptosis of N9 cells in vitro and thus present a potential risk for CNS.
基金financially supported by the Young Scientists Fund of the Natural Science Foundation of Heilongjiang Province (QC2011C099)Scientific Fund of Heilongjiang Province Department of Health (2009-259)+3 种基金Grant from Educational Office of Heilongjiang Province (11551178 and1154HZ11)Fund of Daqing GaoXin Qu (DQGX09YF016)the National Natural Science Foundation of China (30871007)the Natural Science Foundation of Heilongjiang Province (ZD2008-08 and LC2009C12)
文摘A novel vehicle for the delivery of aspirin （ASA） was prepared from porous nano-hydroxyapafite/poly（styrene-divinylbenzene） [nano-HAP/P（St-DVB）] composite microspheres by grafting nano-HAP [Ca10（PO4）6（OH）2] onto porous P（St-DVB） micro- spheres. Four types of porous composite microspheres were prepared, each with different nano-HAP contents. The ASA-loaded composite microspheres prepared with 10% and 15% nano-HAP （mass ratio） exhibited excellent buoyancy with relatively short instantaneous floating time （within l0 min） and a long sustained floating time （12 h） in simulated gastric juice. They also offered good sustained release of ASA （up to 8 h）. Furthermore, these composite microspheres displayed good buff- ering capacity that prevented the buildup of acidity caused by hydrolysis of ASA, keeping the pH of gastric juice within the normal range （pH 0.9 to 1.5）. The results showed that porous nano-HAP/P（St-DVB） composite microspheres prepared with 10% and 15% nano-HAP could be used as a novel drug carrier for ASA, providing a sustained release dose without leading to stomach irritation, a side effect that is often associated with ASA medication.
