目的:研究活化蛋白C(Activated protein C,APC)对大鼠皮瓣缺血再灌注损伤的作用及可能机制。方法:将80只SD雄性大鼠随机分为四组:对照组、药物对照组、模型组和治疗组,每组20只。观察术后72 h内模型大鼠皮瓣形态变化,HE染色观察大鼠皮...目的:研究活化蛋白C(Activated protein C,APC)对大鼠皮瓣缺血再灌注损伤的作用及可能机制。方法:将80只SD雄性大鼠随机分为四组:对照组、药物对照组、模型组和治疗组,每组20只。观察术后72 h内模型大鼠皮瓣形态变化,HE染色观察大鼠皮瓣组织病理学变化,TUNEL法染色观察皮瓣组织细胞凋亡,ELISA法检测血清中TNF-α、IL-6水平,用黄嘌呤氧化酶法和硫代巴比妥酸TBA比色法分别测定皮瓣组织中超氧化物歧化酶(SOD)活性、丙二醛(MDA)含量,Westernblot法检测皮瓣组织中Nrf-2、HO-1、γ-GCS蛋白表达水平。结果:与模型组比较,治疗组皮瓣红肿、坏死程度、病理损伤程度减弱;TUNEL法染色观察,与模型组比较,治疗组皮瓣组织细胞凋亡率减少(P<0.05);ELISA法检测发现,与模型组比较,治疗组血清中TNF-α、IL-6降低(P<0.05);与模型组比较,治疗组SOD活性升高(P<0.05),MDA含量降低(P<0.05);Westernblot法检测发现,与模型组比较,治疗组Nrf-2、HO-1、γ-GCS蛋白相对表达水平上升(P<0.05)。结论:APC能改善大鼠皮瓣缺血再灌注损伤,其机制可能与激活Nrf-2/HO-1信号通路,抑制氧化应激反应和减少细胞凋亡、炎症反应有关。展开更多
In order to optimise land use systems,to prevent erosion-induced degradation and to restore the degraded red soils in subtropical China,five cropping systems and four agrforestry systems were conducted in red soils wi...In order to optimise land use systems,to prevent erosion-induced degradation and to restore the degraded red soils in subtropical China,five cropping systems and four agrforestry systems were conducted in red soils with a slope of 7° from 1993 to 1995,The results showed that erosion risk period occurred from Aproil to June,and the annual runoff and and the losses of soil and nutrients with sediment were alarming for two conventional farming systems,whereas they were negligible for the farming systems with ridge tillage.Enrichment ratios of the lost soils from erosion erer more than 1.20 for all nutrients with much higher values for hydrolysable N and organic matter.Compared with the control,the alley cropping systems also distinctly decreased runoff by 30% or 50%.However,the coverage of soil surface varied with alley cropping systems for the competition of nutrients and soil water,which made a profound difference in runoff.The cropping systems of sweet potato intercropped with soybean,the alley cropping systems and the measures of mulching and ridge tillage were the alternatives for red soil reclamation so as to prevent erosion-induced degradation.展开更多
The main taste components of triploid and diploid oyster (Crassostrea gigas) were compared. Free amino acids, inosine monophosphate, succinate, trimethylamine oxide and betaine in fresh and boiled extractives were ana...The main taste components of triploid and diploid oyster (Crassostrea gigas) were compared. Free amino acids, inosine monophosphate, succinate, trimethylamine oxide and betaine in fresh and boiled extractives were analyzed. The protein, lipids, glycogen, moisture and ash, which may affect the flavour, were evaluated. In boiled extractives, the amino acids were 394.1 mg(100 g) -1 in diploid and 183.5 mg(100 g) -1 in triploid. However, in fresh oyster extractives, they were 320.0 mg(100 g) -1 and 147.3 mg(100 g) -1 respectively. The inosine monophosphate in triploid was 44% more than that in diploid, and a little difference existed in the content of trimethylamine oxide between them. The contents of these taste components were the basis for taste flavour pattern determination.展开更多
Two time-domain reflectometry (TDR) systems and a new impedance measuring instrument, Thetaprobe,which are based on determination of soil dielectric constant, were used to measure water content of clayeyred soil to er...Two time-domain reflectometry (TDR) systems and a new impedance measuring instrument, Thetaprobe,which are based on determination of soil dielectric constant, were used to measure water content of clayeyred soil to eraluate the accuracy of these instruments. The results indicated that these instruments shouldbe carefUlly re-calibrated before being applied in clayey red soil. With a new calibration curve fed into one ofthe TDR systems tested, nase system, the measured data compared well with tho8e by standard oven-dryingmethod.展开更多
The problems existing in the present sampling method for weak layers in exploratory adits are discussed. At present, the confining pressure effect on engineering properties of weak layers is often neglected, but the a...The problems existing in the present sampling method for weak layers in exploratory adits are discussed. At present, the confining pressure effect on engineering properties of weak layers is often neglected, but the argillizational effect under ground water is emphasized. Ordinarily, samples of weak layers are obtained in exploratory adits. But the weak layers are easily affected by the stress relaxation after the excavation of exploratory adits. So the engineering properties of weak layers, based on laboratory or in-situ tests, are very poor. Obviously, the test results of samples from exploratory adits cannot reflect their engineering properties under natural ground stresses. To avoid the effect of stress relaxation on weak layers, a special sampling method in exploratory adits is adopted, which reveals that weak layers are of good engineering properties under confining pressure. Taking the weak intercalations at a giant hydroelectric station on Jinshajiang River as an example, the correlations between physical and mechanical indices and confining pressure indicate that confining pressure is the most important environmental factor to control the engineering properties of weak layers.展开更多
The proton induced X-ray emission(PIXE)is used to measure the chemical ingredients of seven main ele-ments of each sample of celadon body from Qingliangsi kiln in Baofeng County and Zhanggongxiang kiln in Ruzhou City....The proton induced X-ray emission(PIXE)is used to measure the chemical ingredients of seven main ele-ments of each sample of celadon body from Qingliangsi kiln in Baofeng County and Zhanggongxiang kiln in Ruzhou City. The PIXE data are then analyzed by fuzzy cluster method, and the trend cluster diagram is obtained to determine their relation of classification and provenance. The result shows that the producing area of raw material of Ru porcelain bodies is concentrated in Qingliangsi kiln, while that of cela-don bodies is slightly scattered in Zhanggongxiang kiln. The raw material origin of Ru porcelain bodies from Qingliangsi kiln in Baofeng County is different from that of Zhang-gongxiang kiln in Ruzhou City. The materials of Ru Guan porcelain bodies and Ru Civil porcelain bodies in Qingliangsi kiln are basically the same.展开更多
文摘目的:研究活化蛋白C(Activated protein C,APC)对大鼠皮瓣缺血再灌注损伤的作用及可能机制。方法:将80只SD雄性大鼠随机分为四组:对照组、药物对照组、模型组和治疗组,每组20只。观察术后72 h内模型大鼠皮瓣形态变化,HE染色观察大鼠皮瓣组织病理学变化,TUNEL法染色观察皮瓣组织细胞凋亡,ELISA法检测血清中TNF-α、IL-6水平,用黄嘌呤氧化酶法和硫代巴比妥酸TBA比色法分别测定皮瓣组织中超氧化物歧化酶(SOD)活性、丙二醛(MDA)含量,Westernblot法检测皮瓣组织中Nrf-2、HO-1、γ-GCS蛋白表达水平。结果:与模型组比较,治疗组皮瓣红肿、坏死程度、病理损伤程度减弱;TUNEL法染色观察,与模型组比较,治疗组皮瓣组织细胞凋亡率减少(P<0.05);ELISA法检测发现,与模型组比较,治疗组血清中TNF-α、IL-6降低(P<0.05);与模型组比较,治疗组SOD活性升高(P<0.05),MDA含量降低(P<0.05);Westernblot法检测发现,与模型组比较,治疗组Nrf-2、HO-1、γ-GCS蛋白相对表达水平上升(P<0.05)。结论:APC能改善大鼠皮瓣缺血再灌注损伤,其机制可能与激活Nrf-2/HO-1信号通路,抑制氧化应激反应和减少细胞凋亡、炎症反应有关。
文摘In order to optimise land use systems,to prevent erosion-induced degradation and to restore the degraded red soils in subtropical China,five cropping systems and four agrforestry systems were conducted in red soils with a slope of 7° from 1993 to 1995,The results showed that erosion risk period occurred from Aproil to June,and the annual runoff and and the losses of soil and nutrients with sediment were alarming for two conventional farming systems,whereas they were negligible for the farming systems with ridge tillage.Enrichment ratios of the lost soils from erosion erer more than 1.20 for all nutrients with much higher values for hydrolysable N and organic matter.Compared with the control,the alley cropping systems also distinctly decreased runoff by 30% or 50%.However,the coverage of soil surface varied with alley cropping systems for the competition of nutrients and soil water,which made a profound difference in runoff.The cropping systems of sweet potato intercropped with soybean,the alley cropping systems and the measures of mulching and ridge tillage were the alternatives for red soil reclamation so as to prevent erosion-induced degradation.
基金supported by Shandong Natural Science Foundation(No.Y2001D06).
文摘The main taste components of triploid and diploid oyster (Crassostrea gigas) were compared. Free amino acids, inosine monophosphate, succinate, trimethylamine oxide and betaine in fresh and boiled extractives were analyzed. The protein, lipids, glycogen, moisture and ash, which may affect the flavour, were evaluated. In boiled extractives, the amino acids were 394.1 mg(100 g) -1 in diploid and 183.5 mg(100 g) -1 in triploid. However, in fresh oyster extractives, they were 320.0 mg(100 g) -1 and 147.3 mg(100 g) -1 respectively. The inosine monophosphate in triploid was 44% more than that in diploid, and a little difference existed in the content of trimethylamine oxide between them. The contents of these taste components were the basis for taste flavour pattern determination.
文摘Two time-domain reflectometry (TDR) systems and a new impedance measuring instrument, Thetaprobe,which are based on determination of soil dielectric constant, were used to measure water content of clayeyred soil to eraluate the accuracy of these instruments. The results indicated that these instruments shouldbe carefUlly re-calibrated before being applied in clayey red soil. With a new calibration curve fed into one ofthe TDR systems tested, nase system, the measured data compared well with tho8e by standard oven-dryingmethod.
文摘The problems existing in the present sampling method for weak layers in exploratory adits are discussed. At present, the confining pressure effect on engineering properties of weak layers is often neglected, but the argillizational effect under ground water is emphasized. Ordinarily, samples of weak layers are obtained in exploratory adits. But the weak layers are easily affected by the stress relaxation after the excavation of exploratory adits. So the engineering properties of weak layers, based on laboratory or in-situ tests, are very poor. Obviously, the test results of samples from exploratory adits cannot reflect their engineering properties under natural ground stresses. To avoid the effect of stress relaxation on weak layers, a special sampling method in exploratory adits is adopted, which reveals that weak layers are of good engineering properties under confining pressure. Taking the weak intercalations at a giant hydroelectric station on Jinshajiang River as an example, the correlations between physical and mechanical indices and confining pressure indicate that confining pressure is the most important environmental factor to control the engineering properties of weak layers.
文摘The proton induced X-ray emission(PIXE)is used to measure the chemical ingredients of seven main ele-ments of each sample of celadon body from Qingliangsi kiln in Baofeng County and Zhanggongxiang kiln in Ruzhou City. The PIXE data are then analyzed by fuzzy cluster method, and the trend cluster diagram is obtained to determine their relation of classification and provenance. The result shows that the producing area of raw material of Ru porcelain bodies is concentrated in Qingliangsi kiln, while that of cela-don bodies is slightly scattered in Zhanggongxiang kiln. The raw material origin of Ru porcelain bodies from Qingliangsi kiln in Baofeng County is different from that of Zhang-gongxiang kiln in Ruzhou City. The materials of Ru Guan porcelain bodies and Ru Civil porcelain bodies in Qingliangsi kiln are basically the same.
