Seventeen species of Trichoderma, isolated from soil or tree bark from Ch ina are identified based on morphological and physiological characters, and from their phylogenetic position inferred from parsimony analyses o...Seventeen species of Trichoderma, isolated from soil or tree bark from Ch ina are identified based on morphological and physiological characters, and from their phylogenetic position inferred from parsimony analyses of nucleotide sequ ences of the internal transcribed spacer regions of the rDNA cluster (ITS1 and 2) and partial sequences of translation elongation factor 1-alpha (te f1) . There were T.citrinoviride, T.longibrachiatum, T.sinensis in section Long ibrachiatum, T.atroviride, T.koningii, T.viride, T.asperellum, T.hamatum, T.e rinaceum in section Trichoderma, T.harzianum (H.lixii), T.inhamatum, T. ve lutinum, T.cerinum, T.strictipile, T.spirale, T.virens, H.nigrovirens (Trichode r ma sp.) in section Pachybasium. Among them four species: T. asperellum, T .velutinum, T.cerinum, T. spirale were reported firstly in China. In addition, two suspected new taxa (Trichoderma spp.) in Trichoderma s ec tion were proposed: Trichoderma sp.1 (ZAUT261, 4, 4A, 15A, 2C), Trich od erma sp.2 (2B, 5, 7A, 7B, 9A). Trichoderma sp.1 was similar to T.h a matum , but the temperature optimum for mycelial growth was lower than that of T.hamatum and the species tended to form hemisphaerical pustule with rela tively larger conidia (average length 4.6 μm×2.8 μm). Trichoderma sp.2 was distinguished morphologically from related species T. strigosum, T. pub escens, T. erinaceum, T. hamatum and Trichoderma sp.1 in pustules on CM D without fertile or sterile conidiophore elongation and distinctive phialide sh ape, the conidiophore branches similar to T.koningii, but the conidia sim ilar to T. viride, subglobose, conspicuously tuberculate.展开更多
文摘Seventeen species of Trichoderma, isolated from soil or tree bark from Ch ina are identified based on morphological and physiological characters, and from their phylogenetic position inferred from parsimony analyses of nucleotide sequ ences of the internal transcribed spacer regions of the rDNA cluster (ITS1 and 2) and partial sequences of translation elongation factor 1-alpha (te f1) . There were T.citrinoviride, T.longibrachiatum, T.sinensis in section Long ibrachiatum, T.atroviride, T.koningii, T.viride, T.asperellum, T.hamatum, T.e rinaceum in section Trichoderma, T.harzianum (H.lixii), T.inhamatum, T. ve lutinum, T.cerinum, T.strictipile, T.spirale, T.virens, H.nigrovirens (Trichode r ma sp.) in section Pachybasium. Among them four species: T. asperellum, T .velutinum, T.cerinum, T. spirale were reported firstly in China. In addition, two suspected new taxa (Trichoderma spp.) in Trichoderma s ec tion were proposed: Trichoderma sp.1 (ZAUT261, 4, 4A, 15A, 2C), Trich od erma sp.2 (2B, 5, 7A, 7B, 9A). Trichoderma sp.1 was similar to T.h a matum , but the temperature optimum for mycelial growth was lower than that of T.hamatum and the species tended to form hemisphaerical pustule with rela tively larger conidia (average length 4.6 μm×2.8 μm). Trichoderma sp.2 was distinguished morphologically from related species T. strigosum, T. pub escens, T. erinaceum, T. hamatum and Trichoderma sp.1 in pustules on CM D without fertile or sterile conidiophore elongation and distinctive phialide sh ape, the conidiophore branches similar to T.koningii, but the conidia sim ilar to T. viride, subglobose, conspicuously tuberculate.